TY  - JOUR
AU  - Kumar, Vivek
AU  - Sharma, Hemant
AU  - Saini, Lalita
AU  - Tyagi, Archasvi
AU  - Jain, Pooja
AU  - Singh, Yogita
AU  - Balyan, Priyanka
AU  - Kumar, Sachin
AU  - Jan, Sofora
AU  - Mir, Reyazul Rouf
AU  - Đalović, Ivica
AU  - Singh, Krishna Pal
AU  - Kumar, Upendra
AU  - Malik, Vijai
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3203
AB  - The core particle represents the catalytic portions of the 26S proteasomal complex. The genes encoding a- and b-subunits play a crucial role in protecting plants against various environmental stresses by controlling the quality of newly produced proteins. The 20S proteasome gene family has already been reported in model plants such as Arabidopsis and rice; however, they have not been studied in oilseed crops such as rapeseed (Brassica napus L.). In the present study, we identified 20S proteasome genes for a- (PA) and b-subunits (PB) in B. napus through systematically performed gene structure analysis, chromosomal location, conserved motif, phylogenetic relationship, and expression patterns. A total of 82 genes, comprising 35 BnPA and 47 BnPB of the 20S proteasome, were revealed in the B. napus genome. These genes were distributed on all 20 chromosomes of B. napus and most of these genes were duplicated on homoeologous chromosomes. The BnPA (a1-7) and BnPB (b1-7) genes were phylogenetically placed into seven clades. The pattern of expression of all the BnPA and BnPB genes was also studied using RNA-seq datasets under biotic and abiotic stress conditions. Out of 82 BnPA/PB genes, three exhibited high expression under abiotic stresses, whereas two genes were overexpressed in response to biotic stresses at both the seedling and flowering stages. Moreover, an additional eighteen genes were expressed under normal conditions. Overall, the current findings developed our understanding of the organization of the 20S proteasome genes in B. napus and provided specific BnPA/PB genes for further functional research in response to abiotic and biotic stresses.
PB  - Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)
SP  - 1037206
VL  - 13
DO  - 10.3389/fpls.2022.1037206
ER  - 

@article{
author = "Kumar, Vivek and Sharma, Hemant and Saini, Lalita and Tyagi, Archasvi and Jain, Pooja and Singh, Yogita and Balyan, Priyanka and Kumar, Sachin and Jan, Sofora and Mir, Reyazul Rouf and Đalović, Ivica and Singh, Krishna Pal and Kumar, Upendra and Malik, Vijai",
year = "2022",
abstract = "The core particle represents the catalytic portions of the 26S proteasomal complex. The genes encoding a- and b-subunits play a crucial role in protecting plants against various environmental stresses by controlling the quality of newly produced proteins. The 20S proteasome gene family has already been reported in model plants such as Arabidopsis and rice; however, they have not been studied in oilseed crops such as rapeseed (Brassica napus L.). In the present study, we identified 20S proteasome genes for a- (PA) and b-subunits (PB) in B. napus through systematically performed gene structure analysis, chromosomal location, conserved motif, phylogenetic relationship, and expression patterns. A total of 82 genes, comprising 35 BnPA and 47 BnPB of the 20S proteasome, were revealed in the B. napus genome. These genes were distributed on all 20 chromosomes of B. napus and most of these genes were duplicated on homoeologous chromosomes. The BnPA (a1-7) and BnPB (b1-7) genes were phylogenetically placed into seven clades. The pattern of expression of all the BnPA and BnPB genes was also studied using RNA-seq datasets under biotic and abiotic stress conditions. Out of 82 BnPA/PB genes, three exhibited high expression under abiotic stresses, whereas two genes were overexpressed in response to biotic stresses at both the seedling and flowering stages. Moreover, an additional eighteen genes were expressed under normal conditions. Overall, the current findings developed our understanding of the organization of the 20S proteasome genes in B. napus and provided specific BnPA/PB genes for further functional research in response to abiotic and biotic stresses.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)",
pages = "1037206",
volume = "13",
doi = "10.3389/fpls.2022.1037206"
}

Kumar, V., Sharma, H., Saini, L., Tyagi, A., Jain, P., Singh, Y., Balyan, P., Kumar, S., Jan, S., Mir, R. R., Đalović, I., Singh, K. P., Kumar, U.,& Malik, V.. (2022). Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.). in Frontiers in Plant Science
Frontiers Media S.A.., 13, 1037206.
https://doi.org/10.3389/fpls.2022.1037206

Kumar V, Sharma H, Saini L, Tyagi A, Jain P, Singh Y, Balyan P, Kumar S, Jan S, Mir RR, Đalović I, Singh KP, Kumar U, Malik V. Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.). in Frontiers in Plant Science. 2022;13:1037206.
doi:10.3389/fpls.2022.1037206 .

Kumar, Vivek, Sharma, Hemant, Saini, Lalita, Tyagi, Archasvi, Jain, Pooja, Singh, Yogita, Balyan, Priyanka, Kumar, Sachin, Jan, Sofora, Mir, Reyazul Rouf, Đalović, Ivica, Singh, Krishna Pal, Kumar, Upendra, Malik, Vijai, "Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)" in Frontiers in Plant Science, 13 (2022):1037206,
https://doi.org/10.3389/fpls.2022.1037206 . .

TY  - JOUR
AU  - Kumar, Jitendra
AU  - Rouf Mir, Reyazul
AU  - Shafi, Safoora
AU  - Sen Gupta, Debjyoti
AU  - Đalović, Ivica
AU  - Miladinović, Jegor
AU  - Kumar, Rahul
AU  - Kumar, Sachin
AU  - Kumar, Rajeev
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2656
AB  - Cool season grain legumes occupy an important place among the agricultural crops and
essentially provide multiple benefits including food supply, nutrition security, soil fertility improvement and revenue for farmers all over the world. However, owing to climate change, the average temperature is steadily rising, which negatively affects crop performance and limits their yield. Terminal heat stress that mainly occurred during grain development phases severely harms grain quality and weight in legumes adapted to the cool season, such as lentils, faba beans, chickpeas, field peas, etc. Although, traditional breeding approaches with advanced screening procedures have been employed to identify heat tolerant legume cultivars. Unfortunately, traditional breeding pipelines alone are no longer enough to meet global demands. Genomics-assisted interventions including new-generation sequencing technologies and genotyping platforms have facilitated the development of high-resolution molecular maps, QTL/gene discovery and marker-assisted introgression, thereby improving the efficiency in legumes breeding to develop stress-resilient varieties. Based on the current scenario, we attempted to review the intervention of genomics to decipher different components of tolerance to heat stress and future possibilities of using newly developed genomics-based interventions in cool season adapted grain legumes.
PB  - Basel : MDPI
T2  - International Journal of Molecular Sciences
T1  - Genomics associated interventions for heat stress tolerance in cool season adapted grain legumes
SP  - 399
VL  - 23
DO  - 10.3390/ijms23010399
ER  - 

@article{
author = "Kumar, Jitendra and Rouf Mir, Reyazul and Shafi, Safoora and Sen Gupta, Debjyoti and Đalović, Ivica and Miladinović, Jegor and Kumar, Rahul and Kumar, Sachin and Kumar, Rajeev",
year = "2022",
abstract = "Cool season grain legumes occupy an important place among the agricultural crops and
essentially provide multiple benefits including food supply, nutrition security, soil fertility improvement and revenue for farmers all over the world. However, owing to climate change, the average temperature is steadily rising, which negatively affects crop performance and limits their yield. Terminal heat stress that mainly occurred during grain development phases severely harms grain quality and weight in legumes adapted to the cool season, such as lentils, faba beans, chickpeas, field peas, etc. Although, traditional breeding approaches with advanced screening procedures have been employed to identify heat tolerant legume cultivars. Unfortunately, traditional breeding pipelines alone are no longer enough to meet global demands. Genomics-assisted interventions including new-generation sequencing technologies and genotyping platforms have facilitated the development of high-resolution molecular maps, QTL/gene discovery and marker-assisted introgression, thereby improving the efficiency in legumes breeding to develop stress-resilient varieties. Based on the current scenario, we attempted to review the intervention of genomics to decipher different components of tolerance to heat stress and future possibilities of using newly developed genomics-based interventions in cool season adapted grain legumes.",
publisher = "Basel : MDPI",
journal = "International Journal of Molecular Sciences",
title = "Genomics associated interventions for heat stress tolerance in cool season adapted grain legumes",
pages = "399",
volume = "23",
doi = "10.3390/ijms23010399"
}

Kumar, J., Rouf Mir, R., Shafi, S., Sen Gupta, D., Đalović, I., Miladinović, J., Kumar, R., Kumar, S.,& Kumar, R.. (2022). Genomics associated interventions for heat stress tolerance in cool season adapted grain legumes. in International Journal of Molecular Sciences
Basel : MDPI., 23, 399.
https://doi.org/10.3390/ijms23010399

Kumar J, Rouf Mir R, Shafi S, Sen Gupta D, Đalović I, Miladinović J, Kumar R, Kumar S, Kumar R. Genomics associated interventions for heat stress tolerance in cool season adapted grain legumes. in International Journal of Molecular Sciences. 2022;23:399.
doi:10.3390/ijms23010399 .

Kumar, Jitendra, Rouf Mir, Reyazul, Shafi, Safoora, Sen Gupta, Debjyoti, Đalović, Ivica, Miladinović, Jegor, Kumar, Rahul, Kumar, Sachin, Kumar, Rajeev, "Genomics associated interventions for heat stress tolerance in cool season adapted grain legumes" in International Journal of Molecular Sciences, 23 (2022):399,
https://doi.org/10.3390/ijms23010399 . .