TY  - JOUR
AU  - Grahovac, Nada
AU  - Stojanović, Zorica
AU  - Kravić, Snežana
AU  - Orčić, Dejan Z.
AU  - Suturović, Zvonimir
AU  - Kondić-Špika, Ankica
AU  - Vasin, Jovica
AU  - Šunjka, Dragana B.
AU  - Jakšić, Snežana
AU  - Rajković, Miloš
AU  - Grahovac, Nenad M.
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1691
AB  - A modified method for the analysis of nicosulfuron, rimsulfuron and prosulfuron was developed and validated by using microwave-assisted extraction (MAE) and ultra-performance liquid chromatography with diode array detection in the ultraviolet region (HPLC-UV-DAD). The most important experimental parameters of extraction procedure and HPLC-UV-DAD technique were optimised in respect to those sulfonylurea herbicides. High recoveries of the microwave-assisted extraction were obtained by using a dichloromethane-acetonitrile mixture (2:1 volume ratio) acidified with acetic acid (0.8 vol.%) with the addition of urea. The mean recoveries at three spiking levels ranged from 97.47 to 98.76% for nicosulfuron, 97.88 to 99.17% for rimsulfuron and from 97.91 to 99.83% for prosulfuron. The limits of detection of nicosulfuron, rimsulfuron and prosulfuron were 0.95, 0.91 and 0.89 mu g kg(-1), respectively. The accuracy of the developed method was confirmed by HPLC coupled with tandem mass spectrometry parallel analyses. The developed method was used to investigate the dissipation dynamics of sulfonylurea herbicides in the real field trials in Vojvodina Province, Serbia. The obtained half-lives were 0.05, 0.23 and 0.15 days for recommended dose application of nicosulfuron, rimsulfuron and prosulfuron, respectively. Low residues and short half-life in soil suggested that the risk to sensitive rotational crops after application of those sulfonylurea herbicides is low when they are used in the appropriate dosages.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method
EP  - 298
IS  - 4
SP  - 289
VL  - 71
DO  - 10.2298/HEMIND160224039G
ER  - 

@article{
author = "Grahovac, Nada and Stojanović, Zorica and Kravić, Snežana and Orčić, Dejan Z. and Suturović, Zvonimir and Kondić-Špika, Ankica and Vasin, Jovica and Šunjka, Dragana B. and Jakšić, Snežana and Rajković, Miloš and Grahovac, Nenad M.",
year = "2017",
abstract = "A modified method for the analysis of nicosulfuron, rimsulfuron and prosulfuron was developed and validated by using microwave-assisted extraction (MAE) and ultra-performance liquid chromatography with diode array detection in the ultraviolet region (HPLC-UV-DAD). The most important experimental parameters of extraction procedure and HPLC-UV-DAD technique were optimised in respect to those sulfonylurea herbicides. High recoveries of the microwave-assisted extraction were obtained by using a dichloromethane-acetonitrile mixture (2:1 volume ratio) acidified with acetic acid (0.8 vol.%) with the addition of urea. The mean recoveries at three spiking levels ranged from 97.47 to 98.76% for nicosulfuron, 97.88 to 99.17% for rimsulfuron and from 97.91 to 99.83% for prosulfuron. The limits of detection of nicosulfuron, rimsulfuron and prosulfuron were 0.95, 0.91 and 0.89 mu g kg(-1), respectively. The accuracy of the developed method was confirmed by HPLC coupled with tandem mass spectrometry parallel analyses. The developed method was used to investigate the dissipation dynamics of sulfonylurea herbicides in the real field trials in Vojvodina Province, Serbia. The obtained half-lives were 0.05, 0.23 and 0.15 days for recommended dose application of nicosulfuron, rimsulfuron and prosulfuron, respectively. Low residues and short half-life in soil suggested that the risk to sensitive rotational crops after application of those sulfonylurea herbicides is low when they are used in the appropriate dosages.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method",
pages = "298-289",
number = "4",
volume = "71",
doi = "10.2298/HEMIND160224039G"
}

Grahovac, N., Stojanović, Z., Kravić, S., Orčić, D. Z., Suturović, Z., Kondić-Špika, A., Vasin, J., Šunjka, D. B., Jakšić, S., Rajković, M.,& Grahovac, N. M.. (2017). Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 71(4), 289-298.
https://doi.org/10.2298/HEMIND160224039G

Grahovac N, Stojanović Z, Kravić S, Orčić DZ, Suturović Z, Kondić-Špika A, Vasin J, Šunjka DB, Jakšić S, Rajković M, Grahovac NM. Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method. in Hemijska Industrija. 2017;71(4):289-298.
doi:10.2298/HEMIND160224039G .

Grahovac, Nada, Stojanović, Zorica, Kravić, Snežana, Orčić, Dejan Z., Suturović, Zvonimir, Kondić-Špika, Ankica, Vasin, Jovica, Šunjka, Dragana B., Jakšić, Snežana, Rajković, Miloš, Grahovac, Nenad M., "Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method" in Hemijska Industrija, 71, no. 4 (2017):289-298,
https://doi.org/10.2298/HEMIND160224039G . .

TY  - JOUR
AU  - Sibul, Filip
AU  - Orčić, Dejan Z.
AU  - Vasić, Mirjana
AU  - Anačkov, Goran
AU  - Nadpal, Jelena
AU  - Savić, Aleksandra
AU  - Mimica-Dukić, Neda
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1510
AB  - Herb and root parts of legumes are not commonly used in human consumption, since they are traditionally considered waste material. Although inedible, these plant parts have a complex chemical composition, with potential biological activities. They are especially rich in polyphenols, such as flavonoids, isoflavonoids and phenolic acids. In this paper, phenolic profile of the seven selected legumes (soybean (Glycine max L.), common bean (Phaseolus vulgaris L), pea (Pisum sativum L.), broad bean (Vida faba L.), chickpea (Cicer arietinum L), white lupin (Lupinus albus L) and grass pea (Lathyrus sativus L)) was examined using LC-MS/MS method. In the examined herb and root extracts 33 phenolic compounds were identified and quantified. High levels of isoflavones genistein and daidzein were found in G. max, P. vulgaris and L. albus root extracts (1.02-1.53, 0.309-0.648 and 2.81 mg/g, respectively). C. arietinum, L. albus and P. vulgaris herb extracts were found to be the richest sources of flavonoid compounds (13.1-19.4, 5.98 and 2.36-3.64 mg/g, respectively). Since polyphenols have redox properties, antioxidant activity of the abovementioned plants was investigated using DPPH center dot, NO center dot, and FRAP assay. All samples demonstrated the ability to scavenge both DPPH center dot and NO center dot radical, and exhibited a good FRAP reducing power. V. faba exhibited the highest antioxidant activity (DPPH center dot IC50 11.7-20 mu g/mL, NO center dot IC30 0.080-0.34 mg/mL, FRAP 32.6-66.9 AAE/g dw). Anti-inflammatory potential was examined using in vitro COX-1/12-LOX pathway inhibition assay, resulting in moderate activity of all samples (IC50 2.09-8.00 for all metabolites). The obtained results indicate a strong potential of waste legume material as a promising source for isolation of bioactive antioxidant and anti-inflammatory compounds for their use as therapeutic and dietary supplements in pharmaceutical and food industry.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profile, antioxidant and anti-inflammatory potential of herb and root extracts of seven selected legumes
EP  - 653
SP  - 641
VL  - 83
DO  - 10.1016/j.indcrop.2015.12.057
ER  - 

@article{
author = "Sibul, Filip and Orčić, Dejan Z. and Vasić, Mirjana and Anačkov, Goran and Nadpal, Jelena and Savić, Aleksandra and Mimica-Dukić, Neda",
year = "2016",
abstract = "Herb and root parts of legumes are not commonly used in human consumption, since they are traditionally considered waste material. Although inedible, these plant parts have a complex chemical composition, with potential biological activities. They are especially rich in polyphenols, such as flavonoids, isoflavonoids and phenolic acids. In this paper, phenolic profile of the seven selected legumes (soybean (Glycine max L.), common bean (Phaseolus vulgaris L), pea (Pisum sativum L.), broad bean (Vida faba L.), chickpea (Cicer arietinum L), white lupin (Lupinus albus L) and grass pea (Lathyrus sativus L)) was examined using LC-MS/MS method. In the examined herb and root extracts 33 phenolic compounds were identified and quantified. High levels of isoflavones genistein and daidzein were found in G. max, P. vulgaris and L. albus root extracts (1.02-1.53, 0.309-0.648 and 2.81 mg/g, respectively). C. arietinum, L. albus and P. vulgaris herb extracts were found to be the richest sources of flavonoid compounds (13.1-19.4, 5.98 and 2.36-3.64 mg/g, respectively). Since polyphenols have redox properties, antioxidant activity of the abovementioned plants was investigated using DPPH center dot, NO center dot, and FRAP assay. All samples demonstrated the ability to scavenge both DPPH center dot and NO center dot radical, and exhibited a good FRAP reducing power. V. faba exhibited the highest antioxidant activity (DPPH center dot IC50 11.7-20 mu g/mL, NO center dot IC30 0.080-0.34 mg/mL, FRAP 32.6-66.9 AAE/g dw). Anti-inflammatory potential was examined using in vitro COX-1/12-LOX pathway inhibition assay, resulting in moderate activity of all samples (IC50 2.09-8.00 for all metabolites). The obtained results indicate a strong potential of waste legume material as a promising source for isolation of bioactive antioxidant and anti-inflammatory compounds for their use as therapeutic and dietary supplements in pharmaceutical and food industry.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profile, antioxidant and anti-inflammatory potential of herb and root extracts of seven selected legumes",
pages = "653-641",
volume = "83",
doi = "10.1016/j.indcrop.2015.12.057"
}

Sibul, F., Orčić, D. Z., Vasić, M., Anačkov, G., Nadpal, J., Savić, A.,& Mimica-Dukić, N.. (2016). Phenolic profile, antioxidant and anti-inflammatory potential of herb and root extracts of seven selected legumes. in Industrial Crops and Products
Elsevier, Amsterdam., 83, 641-653.
https://doi.org/10.1016/j.indcrop.2015.12.057

Sibul F, Orčić DZ, Vasić M, Anačkov G, Nadpal J, Savić A, Mimica-Dukić N. Phenolic profile, antioxidant and anti-inflammatory potential of herb and root extracts of seven selected legumes. in Industrial Crops and Products. 2016;83:641-653.
doi:10.1016/j.indcrop.2015.12.057 .

Sibul, Filip, Orčić, Dejan Z., Vasić, Mirjana, Anačkov, Goran, Nadpal, Jelena, Savić, Aleksandra, Mimica-Dukić, Neda, "Phenolic profile, antioxidant and anti-inflammatory potential of herb and root extracts of seven selected legumes" in Industrial Crops and Products, 83 (2016):641-653,
https://doi.org/10.1016/j.indcrop.2015.12.057 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Orčić, Dejan Z.
AU  - Sakač, Zvonimir
AU  - Marjanović-Jeromela, Ana
AU  - Grahovac, Nada
AU  - Milošević, Drago M.
AU  - Marisavljević, Dragana P.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1087
AB  - The pathogenicity of phytopathogenic fungi is associated with phytotoxins, especially with their chemical nature and quantity. Sirodesmins are phytotoxins from the epipolythiodioxopiperazines group, produced by the fungus Leptosphaeria maculans, which are a cause of blackleg and stem canker in oilseed rape (Brassica napus L.). The aim of this work was to obtain a detailed chemical profile of sirodesmins in five fungal isolates (four from Vojvodina, Serbia, and one from the Centre for Agricultural Research, Rothamsted, UK). Sirodesmins showing different phytotoxicity on treated cotyledons of cv. Quinta were separated and detected by thin layer chromatography in all analysed isolates (L.m, C-3, St-5 and S-11) except K-113, which neither contained sirodesmin congeners nor did it exhibit activity. By use of high performance liquid chromatography coupled with tandem mass spectrometer, it was possible to identify total of 10 sirodesmins, together with their precursor-phomamide. It was found that the dominant epipolythiodioxopiperazines of the investigated L. maculans isolates were sirodesmin PL, sirodesmin C, and their de-acetylated derivatives.
AB  - Patogenost fitopatogenih gljiva povezana je sa fitotoksinima, a naročito sa njihovom hemijskom prirodom i količinom. Sirodezmini su fitotoksini iz grupe epipolitiodioksopiperazina, koje proizvodi gljiva Leptosphaeria maculans, uzročnik suve truleži korena i raka stabla uljane repice. Cilj ovog rada bila je detaljna hemijska karakterizacija sirodezmina u pet izolata gljiva (četiri iz Vojvodine i jedan iz Velike Britanije, Centar za poljoprivredna istraživanja, Rothamsted). Kod svih ispitivanih izolata (L. maculans, C-3, St-3, S-11), osim K-113 (koji nije sadržao sirodezmine niti pokazivao aktivnost) tankoslojnom hromatografijom su razdvojeni i detektovani sirodezmini koji su pokazali različitu fitotoksičnost na tretiranim kotiledonima sorte Quinta. Primenom tečne hromatografije visoke efikasnosti, kuplovane sa tandemskim masenim spektrometrom, bilo je moguće identifikovati ukupno 10 sirodezmina, kao i njihov prekursor - fomamid. Utvrđeno je da su dominantni epipolitio-dioksopiperazini ispitivanih izolata L. maculans sirodezmin PL, sirodezmin C i njihovi deacetilovani derivati.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of The Serbian Chemical Society
T1  - Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans
T1  - Karakterizacija sirodezmina izolovanih iz fitopatogene gljive Leptosphaeria maculans
EP  - 1379
IS  - 10
SP  - 1363
VL  - 77
DO  - 10.2298/JSC111231048M
ER  - 

@article{
author = "Mitrović, Petar and Orčić, Dejan Z. and Sakač, Zvonimir and Marjanović-Jeromela, Ana and Grahovac, Nada and Milošević, Drago M. and Marisavljević, Dragana P.",
year = "2012",
abstract = "The pathogenicity of phytopathogenic fungi is associated with phytotoxins, especially with their chemical nature and quantity. Sirodesmins are phytotoxins from the epipolythiodioxopiperazines group, produced by the fungus Leptosphaeria maculans, which are a cause of blackleg and stem canker in oilseed rape (Brassica napus L.). The aim of this work was to obtain a detailed chemical profile of sirodesmins in five fungal isolates (four from Vojvodina, Serbia, and one from the Centre for Agricultural Research, Rothamsted, UK). Sirodesmins showing different phytotoxicity on treated cotyledons of cv. Quinta were separated and detected by thin layer chromatography in all analysed isolates (L.m, C-3, St-5 and S-11) except K-113, which neither contained sirodesmin congeners nor did it exhibit activity. By use of high performance liquid chromatography coupled with tandem mass spectrometer, it was possible to identify total of 10 sirodesmins, together with their precursor-phomamide. It was found that the dominant epipolythiodioxopiperazines of the investigated L. maculans isolates were sirodesmin PL, sirodesmin C, and their de-acetylated derivatives., Patogenost fitopatogenih gljiva povezana je sa fitotoksinima, a naročito sa njihovom hemijskom prirodom i količinom. Sirodezmini su fitotoksini iz grupe epipolitiodioksopiperazina, koje proizvodi gljiva Leptosphaeria maculans, uzročnik suve truleži korena i raka stabla uljane repice. Cilj ovog rada bila je detaljna hemijska karakterizacija sirodezmina u pet izolata gljiva (četiri iz Vojvodine i jedan iz Velike Britanije, Centar za poljoprivredna istraživanja, Rothamsted). Kod svih ispitivanih izolata (L. maculans, C-3, St-3, S-11), osim K-113 (koji nije sadržao sirodezmine niti pokazivao aktivnost) tankoslojnom hromatografijom su razdvojeni i detektovani sirodezmini koji su pokazali različitu fitotoksičnost na tretiranim kotiledonima sorte Quinta. Primenom tečne hromatografije visoke efikasnosti, kuplovane sa tandemskim masenim spektrometrom, bilo je moguće identifikovati ukupno 10 sirodezmina, kao i njihov prekursor - fomamid. Utvrđeno je da su dominantni epipolitio-dioksopiperazini ispitivanih izolata L. maculans sirodezmin PL, sirodezmin C i njihovi deacetilovani derivati.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of The Serbian Chemical Society",
title = "Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans, Karakterizacija sirodezmina izolovanih iz fitopatogene gljive Leptosphaeria maculans",
pages = "1379-1363",
number = "10",
volume = "77",
doi = "10.2298/JSC111231048M"
}

Mitrović, P., Orčić, D. Z., Sakač, Z., Marjanović-Jeromela, A., Grahovac, N., Milošević, D. M.,& Marisavljević, D. P.. (2012). Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans. in Journal of The Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(10), 1363-1379.
https://doi.org/10.2298/JSC111231048M

Mitrović P, Orčić DZ, Sakač Z, Marjanović-Jeromela A, Grahovac N, Milošević DM, Marisavljević DP. Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans. in Journal of The Serbian Chemical Society. 2012;77(10):1363-1379.
doi:10.2298/JSC111231048M .

Mitrović, Petar, Orčić, Dejan Z., Sakač, Zvonimir, Marjanović-Jeromela, Ana, Grahovac, Nada, Milošević, Drago M., Marisavljević, Dragana P., "Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans" in Journal of The Serbian Chemical Society, 77, no. 10 (2012):1363-1379,
https://doi.org/10.2298/JSC111231048M . .