TY  - CONF
AU  - Romanić, R
AU  - Lužaić, Tanja
AU  - Kravić, S.
AU  - Stojanović, Z.
AU  - Grahovac, Nada
AU  - Cvejić, Sandra
AU  - Jocić, Siniša
AU  - Šunjka, D.
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/3707
AB  - Oxidative stability, i.c. shelf life of oil is related to the degree of the oxidative changes
in the amount of the resulting primary and secondary oxidation products of unsaturated fatty
acids. In order to improve oxidative stability, i.e. oil shelf life, sunflower hybrids with altered
fatty acid composition have been created, i.e. significantly higher oleic acid (C18:1) content,
even over 90% w/w, relative to the linoleic type with 25-30% w/w of oleic and 60-65% w/w
of linoleic acid (C18:2). In order to examine the oxidative stability of the oil, three samples of
sunflower seed oil NS hybrids (NS Oliva, NS Horizont and NS Romeo) of different fatty acid
composition were analyzed. Samples were exposed to moderate temperatures (6342°C) over a
period of 8 days. Changes in the content of primary and secondary oxidation products based
on changes in peroxide (PV) and anisidine (p-AnV) values, as well as changes in the content
of conjugated dienes and trienes, were observed. The highest oxidative stability was observed
in the oleic type oil sample. In this sample the determined values of the tested parameters of
oxidative stability, after 8 days, were PV=4.85 mmol/kg and p-AnV=0.65, compared to the
initial sample (PV=0.36 mmol/kg and p-AnV=0.57). The greatest oxidative changes occurred
in the oil sample with the lowest content of oleic and the highest content of linoleic acid, as
indicated by the PV=73.22 mmol/kg and p-AnV=3.60, after 8 days, in relation to PV=2.16
mmol/kg and p-AnV=0.50 in the initial linoleic type oil sample.
C3  - 9th International Scientific Agricultural Symposium “Agrosym 2018”, Jahorina, Bosnia and Herzegovina
T1  - Investigation of content of primary and secondary oxidation products in sunflower oils with a different content of oleic acid
EP  - 339
SP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3707
ER  - 

@conference{
author = "Romanić, R and Lužaić, Tanja and Kravić, S. and Stojanović, Z. and Grahovac, Nada and Cvejić, Sandra and Jocić, Siniša and Šunjka, D.",
year = "2018",
abstract = "Oxidative stability, i.c. shelf life of oil is related to the degree of the oxidative changes
in the amount of the resulting primary and secondary oxidation products of unsaturated fatty
acids. In order to improve oxidative stability, i.e. oil shelf life, sunflower hybrids with altered
fatty acid composition have been created, i.e. significantly higher oleic acid (C18:1) content,
even over 90% w/w, relative to the linoleic type with 25-30% w/w of oleic and 60-65% w/w
of linoleic acid (C18:2). In order to examine the oxidative stability of the oil, three samples of
sunflower seed oil NS hybrids (NS Oliva, NS Horizont and NS Romeo) of different fatty acid
composition were analyzed. Samples were exposed to moderate temperatures (6342°C) over a
period of 8 days. Changes in the content of primary and secondary oxidation products based
on changes in peroxide (PV) and anisidine (p-AnV) values, as well as changes in the content
of conjugated dienes and trienes, were observed. The highest oxidative stability was observed
in the oleic type oil sample. In this sample the determined values of the tested parameters of
oxidative stability, after 8 days, were PV=4.85 mmol/kg and p-AnV=0.65, compared to the
initial sample (PV=0.36 mmol/kg and p-AnV=0.57). The greatest oxidative changes occurred
in the oil sample with the lowest content of oleic and the highest content of linoleic acid, as
indicated by the PV=73.22 mmol/kg and p-AnV=3.60, after 8 days, in relation to PV=2.16
mmol/kg and p-AnV=0.50 in the initial linoleic type oil sample.",
journal = "9th International Scientific Agricultural Symposium “Agrosym 2018”, Jahorina, Bosnia and Herzegovina",
title = "Investigation of content of primary and secondary oxidation products in sunflower oils with a different content of oleic acid",
pages = "339-339",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3707"
}

Romanić, R., Lužaić, T., Kravić, S., Stojanović, Z., Grahovac, N., Cvejić, S., Jocić, S.,& Šunjka, D.. (2018). Investigation of content of primary and secondary oxidation products in sunflower oils with a different content of oleic acid. in 9th International Scientific Agricultural Symposium “Agrosym 2018”, Jahorina, Bosnia and Herzegovina, 339-339.
https://hdl.handle.net/21.15107/rcub_fiver_3707

Romanić R, Lužaić T, Kravić S, Stojanović Z, Grahovac N, Cvejić S, Jocić S, Šunjka D. Investigation of content of primary and secondary oxidation products in sunflower oils with a different content of oleic acid. in 9th International Scientific Agricultural Symposium “Agrosym 2018”, Jahorina, Bosnia and Herzegovina. 2018;:339-339.
https://hdl.handle.net/21.15107/rcub_fiver_3707 .

Romanić, R, Lužaić, Tanja, Kravić, S., Stojanović, Z., Grahovac, Nada, Cvejić, Sandra, Jocić, Siniša, Šunjka, D., "Investigation of content of primary and secondary oxidation products in sunflower oils with a different content of oleic acid" in 9th International Scientific Agricultural Symposium “Agrosym 2018”, Jahorina, Bosnia and Herzegovina (2018):339-339,
https://hdl.handle.net/21.15107/rcub_fiver_3707 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Šunjka, D.
AU  - Lazić, S.
AU  - Vasin, Jovica
AU  - Kondić-Špika, Ankica
AU  - Jakšić, Snežana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/3702
AB  - Sulfunyluares (SUs) present a new generation of herbicides which selectively control broad- and narrow-leaved weeds. Nonethel iiened utilization of SUs leads to increased concern about herbicides residues as the « msequence of their prolonged persistence in some soils. Nicosulfuron belongs to the group of SUs herbicide. A laboratory experiment at termostat (temperature of 25°C) was carried out to evaluate the effect of temn< ‘ture on the dissipation dynamics and final residues of nicosulfuron in soil type Calcie Chernozem Clayic, Pachic. The dissipation of nicosulfuron in soi! was described by using the Mittag-Leffler function ¢ E, (-hr), ere obtained from the experimental data. The residues of nicosulfuron
iphy with diode array detection in the cted herbicide was applied as aqueous solution of commercial formulation nicosulfuron (K elvin", 40 ¢ L'). The starting coneentrations of nicosulfuron (measured in the samples soil taken on the ( th day, ! houirs afier the treatment)
Sulfunyluares (SUs) pre 1 new
 Model coefficients a, h. « v
were determined by uitra-performance liquid chromatog ultraviolet region. In order to invest gate dissipation
 
rye cs on 25°C were 389.84 ug ke ‘9.18 = : | ee E ¢ coe . pg kg, 649.02 ug kg 'and 797.69 ug kg! after the treatment with 40 grams active ingredient (a.i.) per hectare of nicosulfuron ig ai. ha'), 50 gal. ha”, 80.g ai. ha” and 100 gai. ha’. respectively. Atier two days, residues of nicosulfuron were lower more than 86% compared with the initial concentra ‘on. Pifty aays aficr the application of all doses the content of residues were below the LOQ for nicosulfuron (3.16 Hg kg'). The obtained half-time degradation (DT:») values of nicosulfuron vere in range of 0.34 d (80 g a.i. ha’ ') to 0.68 d (40 £att Significant reater Cissipation of nicosulfuron observed in this laboratory testing was influenced, most probably, by abiouc and biotic processes. Nicosulfuron poses low risks to the . imilar type soil because of their rapid dissipation,
C3  - 8th International Scientific Agriculture Symposium “AGROSYM 2017”,2017, Jahorina, Bosnia and Herzegovina
T1  - Disappearance of nicosulfuron residues in soil under laboratory conditions
SP  - 889
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3702
ER  - 

@conference{
author = "Grahovac, Nada and Šunjka, D. and Lazić, S. and Vasin, Jovica and Kondić-Špika, Ankica and Jakšić, Snežana",
year = "2017",
abstract = "Sulfunyluares (SUs) present a new generation of herbicides which selectively control broad- and narrow-leaved weeds. Nonethel iiened utilization of SUs leads to increased concern about herbicides residues as the « msequence of their prolonged persistence in some soils. Nicosulfuron belongs to the group of SUs herbicide. A laboratory experiment at termostat (temperature of 25°C) was carried out to evaluate the effect of temn< ‘ture on the dissipation dynamics and final residues of nicosulfuron in soil type Calcie Chernozem Clayic, Pachic. The dissipation of nicosulfuron in soi! was described by using the Mittag-Leffler function ¢ E, (-hr), ere obtained from the experimental data. The residues of nicosulfuron
iphy with diode array detection in the cted herbicide was applied as aqueous solution of commercial formulation nicosulfuron (K elvin", 40 ¢ L'). The starting coneentrations of nicosulfuron (measured in the samples soil taken on the ( th day, ! houirs afier the treatment)
Sulfunyluares (SUs) pre 1 new
 Model coefficients a, h. « v
were determined by uitra-performance liquid chromatog ultraviolet region. In order to invest gate dissipation
 
rye cs on 25°C were 389.84 ug ke ‘9.18 = : | ee E ¢ coe . pg kg, 649.02 ug kg 'and 797.69 ug kg! after the treatment with 40 grams active ingredient (a.i.) per hectare of nicosulfuron ig ai. ha'), 50 gal. ha”, 80.g ai. ha” and 100 gai. ha’. respectively. Atier two days, residues of nicosulfuron were lower more than 86% compared with the initial concentra ‘on. Pifty aays aficr the application of all doses the content of residues were below the LOQ for nicosulfuron (3.16 Hg kg'). The obtained half-time degradation (DT:») values of nicosulfuron vere in range of 0.34 d (80 g a.i. ha’ ') to 0.68 d (40 £att Significant reater Cissipation of nicosulfuron observed in this laboratory testing was influenced, most probably, by abiouc and biotic processes. Nicosulfuron poses low risks to the . imilar type soil because of their rapid dissipation,",
journal = "8th International Scientific Agriculture Symposium “AGROSYM 2017”,2017, Jahorina, Bosnia and Herzegovina",
title = "Disappearance of nicosulfuron residues in soil under laboratory conditions",
pages = "889",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3702"
}

Grahovac, N., Šunjka, D., Lazić, S., Vasin, J., Kondić-Špika, A.,& Jakšić, S.. (2017). Disappearance of nicosulfuron residues in soil under laboratory conditions. in 8th International Scientific Agriculture Symposium “AGROSYM 2017”,2017, Jahorina, Bosnia and Herzegovina, 889.
https://hdl.handle.net/21.15107/rcub_fiver_3702

Grahovac N, Šunjka D, Lazić S, Vasin J, Kondić-Špika A, Jakšić S. Disappearance of nicosulfuron residues in soil under laboratory conditions. in 8th International Scientific Agriculture Symposium “AGROSYM 2017”,2017, Jahorina, Bosnia and Herzegovina. 2017;:889.
https://hdl.handle.net/21.15107/rcub_fiver_3702 .

Grahovac, Nada, Šunjka, D., Lazić, S., Vasin, Jovica, Kondić-Špika, Ankica, Jakšić, Snežana, "Disappearance of nicosulfuron residues in soil under laboratory conditions" in 8th International Scientific Agriculture Symposium “AGROSYM 2017”,2017, Jahorina, Bosnia and Herzegovina (2017):889,
https://hdl.handle.net/21.15107/rcub_fiver_3702 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Kondić-Špika, Ankica
AU  - Sakač, Zvonimir
AU  - Marjanović-Jeromela, Ana
AU  - Hristov, N.
AU  - Krstović, S.
AU  - Šunjka, D.
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/3721
AB  - Wheat bran is specifically rich in dietary fibre, essential fatty acids and contains important quantities of starch, protein, vitamins, dietary minerals and phytic acid. It is also well known medicinally for its powerful anticarcinogenic and antioxidant properties. Wheat bran is rich source of essential fatty acids, such as linoleic acid (18:2n6) and linolenic acid (18:3n3). Inoleic acid and fatty acid of n6 and n3 series play an important role in the odulation of human metabolism. Linoleic acid is undoubtedly one of the most location of Rimski Sančevi (Vojvodina Province, Serbia) to evaluate oil tents and fatty acid profiles in a collection of 25 wheat genotypes.
PB  - Novi Sad : University of Novi Sad, Institute of Food Technology
C3  - 3rd International Congress „Food Technology, Quality and Safety” and XVII International Symposium „Feed Technology” (FoodTech2016), Novi Sad, 25 October 2016
T1  - Determination of fatty acid composition in wheat bran by gas chromatography
EP  - 50
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3721
ER  - 

@conference{
author = "Grahovac, Nada and Kondić-Špika, Ankica and Sakač, Zvonimir and Marjanović-Jeromela, Ana and Hristov, N. and Krstović, S. and Šunjka, D.",
year = "2016",
abstract = "Wheat bran is specifically rich in dietary fibre, essential fatty acids and contains important quantities of starch, protein, vitamins, dietary minerals and phytic acid. It is also well known medicinally for its powerful anticarcinogenic and antioxidant properties. Wheat bran is rich source of essential fatty acids, such as linoleic acid (18:2n6) and linolenic acid (18:3n3). Inoleic acid and fatty acid of n6 and n3 series play an important role in the odulation of human metabolism. Linoleic acid is undoubtedly one of the most location of Rimski Sančevi (Vojvodina Province, Serbia) to evaluate oil tents and fatty acid profiles in a collection of 25 wheat genotypes.",
publisher = "Novi Sad : University of Novi Sad, Institute of Food Technology",
journal = "3rd International Congress „Food Technology, Quality and Safety” and XVII International Symposium „Feed Technology” (FoodTech2016), Novi Sad, 25 October 2016",
title = "Determination of fatty acid composition in wheat bran by gas chromatography",
pages = "50-50",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3721"
}

Grahovac, N., Kondić-Špika, A., Sakač, Z., Marjanović-Jeromela, A., Hristov, N., Krstović, S.,& Šunjka, D.. (2016). Determination of fatty acid composition in wheat bran by gas chromatography. in 3rd International Congress „Food Technology, Quality and Safety” and XVII International Symposium „Feed Technology” (FoodTech2016), Novi Sad, 25 October 2016
Novi Sad : University of Novi Sad, Institute of Food Technology., 50-50.
https://hdl.handle.net/21.15107/rcub_fiver_3721

Grahovac N, Kondić-Špika A, Sakač Z, Marjanović-Jeromela A, Hristov N, Krstović S, Šunjka D. Determination of fatty acid composition in wheat bran by gas chromatography. in 3rd International Congress „Food Technology, Quality and Safety” and XVII International Symposium „Feed Technology” (FoodTech2016), Novi Sad, 25 October 2016. 2016;:50-50.
https://hdl.handle.net/21.15107/rcub_fiver_3721 .

Grahovac, Nada, Kondić-Špika, Ankica, Sakač, Zvonimir, Marjanović-Jeromela, Ana, Hristov, N., Krstović, S., Šunjka, D., "Determination of fatty acid composition in wheat bran by gas chromatography" in 3rd International Congress „Food Technology, Quality and Safety” and XVII International Symposium „Feed Technology” (FoodTech2016), Novi Sad, 25 October 2016 (2016):50-50,
https://hdl.handle.net/21.15107/rcub_fiver_3721 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Suturović, Z
AU  - Kondić-Špika, Ankica
AU  - Sekulić, P.
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3731
AB  - Sulfonylureas represent a major advancement in global crop protection technology
and they have revolutionized weed control by introducing a unique mode of action.
These herbicides have low toxicity to mammals while they are highly toxic to plants.
The fate of sulfonylurea herbicides is directly related to their chemical structure and
mainly to the ionisation of the sulfonylurea bridge. The dissipation behavior of
nicosulfuron and oxasulfuron in soil were investigated. Soil was cored on
experimental site Rimski Sanéevi in Serbia four times (0-30 days after the spray).
The residue levels and dissipation rates of nicosulfuron and oxasulfuron in soil were
determined by liquid chromatography with diode array detection. The pesticide
formulations were applied in two different rates for nicosulfuron (50 g ai ha-1 and
100 g ai ha Nicogan 40SC) and oxasulfuron (80 g ai ha’' and 160 g ai ha’' Dynox).
The fortified recoveries were in the following range for nicosulfuron from 85.90% to
98.71% with relative standard deviations (RSDs) of 0.16—4.80% and for oxasulfuron
from 88.09% to 99.01% with relative standard deviations (RSDs) of 1.6-5.66%. The
limit of detections (LODs) for nicosulfuron and oxasulfuron in soil were 0.002 mg/kg
and 0.003 mg/kg, respectively. The dissipation of nicosulfuron and oxasulfuron
residues over the time in soil was described by the Mittag-Leffler function a*Ea,B (-
bt). Coefficients a, b, a, 8 were obtained from the experimental data, by using fitting
procedure. We got a=0.8, B=3.71, a=159.11, b=12.79 for nicosulfuron and a=0.8,
B=4.11, a=82.53, b=7.0 for oxasulfuron. The field dissipation half-life time for
nicosulfuron at the topsoil (0-30cm soil depth) was 5.2 days while for oxasulfuron
was 9.67days after application. These results could be utilized for the environmental
risks assessment and minimizing risk for contamination of natural water resources
and damage to following crops.
C3  - 8th International Symposium of Pesticides in Food and the Environment in Mediterranean Countries, Cappadocia (Urgup)/Nevsehir, Turkey
T1  - Dissipation of nicosulfuron and oxasulfuron in soil under field condition
EP  - 105
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3731
ER  - 

@conference{
author = "Grahovac, Nada and Suturović, Z and Kondić-Špika, Ankica and Sekulić, P. and Lazić, S. and Šunjka, D. and Jakšić, Snežana",
year = "2013",
abstract = "Sulfonylureas represent a major advancement in global crop protection technology
and they have revolutionized weed control by introducing a unique mode of action.
These herbicides have low toxicity to mammals while they are highly toxic to plants.
The fate of sulfonylurea herbicides is directly related to their chemical structure and
mainly to the ionisation of the sulfonylurea bridge. The dissipation behavior of
nicosulfuron and oxasulfuron in soil were investigated. Soil was cored on
experimental site Rimski Sanéevi in Serbia four times (0-30 days after the spray).
The residue levels and dissipation rates of nicosulfuron and oxasulfuron in soil were
determined by liquid chromatography with diode array detection. The pesticide
formulations were applied in two different rates for nicosulfuron (50 g ai ha-1 and
100 g ai ha Nicogan 40SC) and oxasulfuron (80 g ai ha’' and 160 g ai ha’' Dynox).
The fortified recoveries were in the following range for nicosulfuron from 85.90% to
98.71% with relative standard deviations (RSDs) of 0.16—4.80% and for oxasulfuron
from 88.09% to 99.01% with relative standard deviations (RSDs) of 1.6-5.66%. The
limit of detections (LODs) for nicosulfuron and oxasulfuron in soil were 0.002 mg/kg
and 0.003 mg/kg, respectively. The dissipation of nicosulfuron and oxasulfuron
residues over the time in soil was described by the Mittag-Leffler function a*Ea,B (-
bt). Coefficients a, b, a, 8 were obtained from the experimental data, by using fitting
procedure. We got a=0.8, B=3.71, a=159.11, b=12.79 for nicosulfuron and a=0.8,
B=4.11, a=82.53, b=7.0 for oxasulfuron. The field dissipation half-life time for
nicosulfuron at the topsoil (0-30cm soil depth) was 5.2 days while for oxasulfuron
was 9.67days after application. These results could be utilized for the environmental
risks assessment and minimizing risk for contamination of natural water resources
and damage to following crops.",
journal = "8th International Symposium of Pesticides in Food and the Environment in Mediterranean Countries, Cappadocia (Urgup)/Nevsehir, Turkey",
title = "Dissipation of nicosulfuron and oxasulfuron in soil under field condition",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3731"
}

Grahovac, N., Suturović, Z., Kondić-Špika, A., Sekulić, P., Lazić, S., Šunjka, D.,& Jakšić, S.. (2013). Dissipation of nicosulfuron and oxasulfuron in soil under field condition. in 8th International Symposium of Pesticides in Food and the Environment in Mediterranean Countries, Cappadocia (Urgup)/Nevsehir, Turkey, 105-105.
https://hdl.handle.net/21.15107/rcub_fiver_3731

Grahovac N, Suturović Z, Kondić-Špika A, Sekulić P, Lazić S, Šunjka D, Jakšić S. Dissipation of nicosulfuron and oxasulfuron in soil under field condition. in 8th International Symposium of Pesticides in Food and the Environment in Mediterranean Countries, Cappadocia (Urgup)/Nevsehir, Turkey. 2013;:105-105.
https://hdl.handle.net/21.15107/rcub_fiver_3731 .

Grahovac, Nada, Suturović, Z, Kondić-Špika, Ankica, Sekulić, P., Lazić, S., Šunjka, D., Jakšić, Snežana, "Dissipation of nicosulfuron and oxasulfuron in soil under field condition" in 8th International Symposium of Pesticides in Food and the Environment in Mediterranean Countries, Cappadocia (Urgup)/Nevsehir, Turkey (2013):105-105,
https://hdl.handle.net/21.15107/rcub_fiver_3731 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Kondić-Špika, Ankica
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Jakšić, Snežana
AU  - Radović, B.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3723
AB  - Residues of active substances, which were frequently found in fruits and vegetables of market producers belonged to the dithiocarbamate group pesticide. According to their use dithiocarbamates belong to fungicides in terms dithiocarbamic acid. They are a group of compounds that exist as strong complexes with various metal ions, often in a polymeric form. This makes them difficult to analyse directly because of their limited solubility in most organic solvents. It is important to have a reliable method which enables the quick and simple detection of this group for official monitoring. This work reports a simple, rapid and sensitive method for the assessment residues dithiocarbamates in fruits. Dithiocarbamates were determined indirectly by measuring the amount of carbon disulfide (CS>) that is liberated by the chemical reaction. The fruit samples in undisturbed condition were heated with a solution of stannous (II) chloride and hydrochloric acid yielding carbon disulphide. Incurred carbon disulfide (CS) are determined "head-space" technique of gas-chromatography with mass detector. Linearity was verified by using the solutions of carbon disulphide in acetone. A linear dynamic range was obtained over a range of concentrations from 0.02 to 0.12 mg/kg for carbon disulfide with correlation coefficient r > 0.995. The accuracy of the method was acceptable since the average recoveries measured at four fortification levels were in the range of 83-103% (n = 4). The precision of the developed procedure expressed as the relative standard deviations (RSDs) were lower than 3.7% in all cases. Quantification was based on external standard calibration curves made with spiked blankmatrices.
PB  - Nacionalno drugtvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
C3  - 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013
T1  - Validation of the method for the determination of dithiocarbamates in fruits
EP  - 292
SP  - 292
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3723
ER  - 

@conference{
author = "Grahovac, Nada and Kondić-Špika, Ankica and Lazić, S. and Šunjka, D. and Jakšić, Snežana and Radović, B.",
year = "2013",
abstract = "Residues of active substances, which were frequently found in fruits and vegetables of market producers belonged to the dithiocarbamate group pesticide. According to their use dithiocarbamates belong to fungicides in terms dithiocarbamic acid. They are a group of compounds that exist as strong complexes with various metal ions, often in a polymeric form. This makes them difficult to analyse directly because of their limited solubility in most organic solvents. It is important to have a reliable method which enables the quick and simple detection of this group for official monitoring. This work reports a simple, rapid and sensitive method for the assessment residues dithiocarbamates in fruits. Dithiocarbamates were determined indirectly by measuring the amount of carbon disulfide (CS>) that is liberated by the chemical reaction. The fruit samples in undisturbed condition were heated with a solution of stannous (II) chloride and hydrochloric acid yielding carbon disulphide. Incurred carbon disulfide (CS) are determined "head-space" technique of gas-chromatography with mass detector. Linearity was verified by using the solutions of carbon disulphide in acetone. A linear dynamic range was obtained over a range of concentrations from 0.02 to 0.12 mg/kg for carbon disulfide with correlation coefficient r > 0.995. The accuracy of the method was acceptable since the average recoveries measured at four fortification levels were in the range of 83-103% (n = 4). The precision of the developed procedure expressed as the relative standard deviations (RSDs) were lower than 3.7% in all cases. Quantification was based on external standard calibration curves made with spiked blankmatrices.",
publisher = "Nacionalno drugtvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013",
title = "Validation of the method for the determination of dithiocarbamates in fruits",
pages = "292-292",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3723"
}

Grahovac, N., Kondić-Špika, A., Lazić, S., Šunjka, D., Jakšić, S.,& Radović, B.. (2013). Validation of the method for the determination of dithiocarbamates in fruits. in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013
Nacionalno drugtvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 292-292.
https://hdl.handle.net/21.15107/rcub_fiver_3723

Grahovac N, Kondić-Špika A, Lazić S, Šunjka D, Jakšić S, Radović B. Validation of the method for the determination of dithiocarbamates in fruits. in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013. 2013;:292-292.
https://hdl.handle.net/21.15107/rcub_fiver_3723 .

Grahovac, Nada, Kondić-Špika, Ankica, Lazić, S., Šunjka, D., Jakšić, Snežana, Radović, B., "Validation of the method for the determination of dithiocarbamates in fruits" in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013 (2013):292-292,
https://hdl.handle.net/21.15107/rcub_fiver_3723 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Kondić-Špika, Ankica
AU  - Suturović, Z.
AU  - Sekulić, P.
AU  - Šunjka, D.
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3734
AB  - Zemijište predstavlja glavni recipijent u kome se adsorbuju i razgrađuju pesticidi, koji u njega dospevaju direktnim putem, kao posledica kontrolisane primene pri suzbijanju korova. Nikosulfuron je selektivni sistematični herbicid, apsorbuje se listom i korenom, relativno brzo se prenosi do meristemskih tkiva. Ovaj herbicid inhibira sintezu acetolaktat sintetazu (ALS), ključni enizim koji učestvuje u biosintezi amino-kiselina sa razgranatim alifatičnim nizom (valin, leucin i izoleucin) u biljkama i zaustavlja deobu ćelija i rast biljke. Nikosulfuron se primenjuje nakon nicanja kukuruza za suzbijanje jednogodišnjih i višegodišnjih uskolisnih i širokolisnih travnih korova. U poređenju sa tradicionalnim herbicidima koji se primenjuju u količini >1kg/ha, nikosulfuron je efikasan u malim količinama <100 g/ha. Postojanost nikosulfurona u zemljištu zavisi od pH, temperature, vlažnosti zemljišta i sadržaja organske materije u zemljištu. S porastom temperature ubrzava se razlaganje nikosulfurona, kako pod uticajem hemijskih faktora (hidroliza, oksidacija) tako i pod uticajem mikroorganizama.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“ Vršac, 21-24. maj 2013.
T1  - Ekstrakcija nikosulfurona iz zemljišta
EP  - 393
SP  - 392
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3734
ER  - 

@conference{
author = "Grahovac, Nada and Kondić-Špika, Ankica and Suturović, Z. and Sekulić, P. and Šunjka, D. and Jakšić, Snežana",
year = "2013",
abstract = "Zemijište predstavlja glavni recipijent u kome se adsorbuju i razgrađuju pesticidi, koji u njega dospevaju direktnim putem, kao posledica kontrolisane primene pri suzbijanju korova. Nikosulfuron je selektivni sistematični herbicid, apsorbuje se listom i korenom, relativno brzo se prenosi do meristemskih tkiva. Ovaj herbicid inhibira sintezu acetolaktat sintetazu (ALS), ključni enizim koji učestvuje u biosintezi amino-kiselina sa razgranatim alifatičnim nizom (valin, leucin i izoleucin) u biljkama i zaustavlja deobu ćelija i rast biljke. Nikosulfuron se primenjuje nakon nicanja kukuruza za suzbijanje jednogodišnjih i višegodišnjih uskolisnih i širokolisnih travnih korova. U poređenju sa tradicionalnim herbicidima koji se primenjuju u količini >1kg/ha, nikosulfuron je efikasan u malim količinama <100 g/ha. Postojanost nikosulfurona u zemljištu zavisi od pH, temperature, vlažnosti zemljišta i sadržaja organske materije u zemljištu. S porastom temperature ubrzava se razlaganje nikosulfurona, kako pod uticajem hemijskih faktora (hidroliza, oksidacija) tako i pod uticajem mikroorganizama.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“ Vršac, 21-24. maj 2013.",
title = "Ekstrakcija nikosulfurona iz zemljišta",
pages = "393-392",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3734"
}

Grahovac, N., Kondić-Špika, A., Suturović, Z., Sekulić, P., Šunjka, D.,& Jakšić, S.. (2013). Ekstrakcija nikosulfurona iz zemljišta. in Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“ Vršac, 21-24. maj 2013.
Beograd : Srpsko hemijsko društvo., 392-393.
https://hdl.handle.net/21.15107/rcub_fiver_3734

Grahovac N, Kondić-Špika A, Suturović Z, Sekulić P, Šunjka D, Jakšić S. Ekstrakcija nikosulfurona iz zemljišta. in Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“ Vršac, 21-24. maj 2013.. 2013;:392-393.
https://hdl.handle.net/21.15107/rcub_fiver_3734 .

Grahovac, Nada, Kondić-Špika, Ankica, Suturović, Z., Sekulić, P., Šunjka, D., Jakšić, Snežana, "Ekstrakcija nikosulfurona iz zemljišta" in Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“ Vršac, 21-24. maj 2013. (2013):392-393,
https://hdl.handle.net/21.15107/rcub_fiver_3734 .

TY  - CONF
AU  - Jakšić, Snežana
AU  - Vasin, Jovica
AU  - Grahovac, Nada
AU  - Popović, Vera
AU  - Šunjka, D.
AU  - Mijić, B.
AU  - Komlen, V.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3722
AB  - Increased concentrations of heavy metals in some agricultural areas, primarily as a result of human
influence, threaten, among other things, the production of high-quality feed. Medicago sativa Lis
(alfalfa) is a perennial legume, which is regarded as the leading and most important forage crop for the
production of quality feed and used in fresh and canned condition as hay, haylage, silage, meal, pellets
and paste. The aim of this study was to determine the levels of mercury in soil and plants Medicago
sativa L.. to obtain information on safety of this nutrient. The examination was conducted in Hrtkovei,
in the Republic of Serbia. Examined soil belongs to the automorphic soils, humus-accumulative class,
type of chernozem, subtypes on loess and loess-like sediments and variety gleyic chernozem, with
profile Ajno-AC-C. Soil has a good physical and chemical properties and is suitable for the production
of alfalfa, if contamination with heavy metals would not be a limiting factor. The study involved eight
localities. Sampling of soil and plant material was carried out in May 2011., in the second production
year of alfalfa. Soil samples were taken in a disrupted state with agrochemical probe, at a depth of 0-30
cm. Mercury content was determined by cold vapor technique, using atomic absorption
spectrophotometer. The mercury content in soil samples of examined localities was below the 0.001
mg/kg, which was not exceeded maximum level of 2.0 mg/kg, according to the Regulations on
permitted amounts of hazardous and harmful substances in soil and water for irrigation and methods of
their analysis (Slu%beni glasnik RS, no. 23/1994). The content of mercury in Medicago sativa L. was
below 0.001 mg/kg, which is below the maximum permitted concentration, according to the
Regulations on the quality of the feed (Sluzbeni glasnik RS, no. 4/2010) is 0.1 mg/kg. On the basis of
these results it can be concluded that the examined samples of alfalfa are suitable for feeding animals
and do not threaten the food chain, as well as safe food production.
PB  - National Society of Processing and Energy in Agriculture, Novi Sad, Serbia
C3  - 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEPl, Vrnjačka Banja, 21 April 2013
T1  - The content of mercury in alfaalfa samples from gleyic chernozem
EP  - 298
SP  - 297
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3722
ER  - 

@conference{
author = "Jakšić, Snežana and Vasin, Jovica and Grahovac, Nada and Popović, Vera and Šunjka, D. and Mijić, B. and Komlen, V.",
year = "2013",
abstract = "Increased concentrations of heavy metals in some agricultural areas, primarily as a result of human
influence, threaten, among other things, the production of high-quality feed. Medicago sativa Lis
(alfalfa) is a perennial legume, which is regarded as the leading and most important forage crop for the
production of quality feed and used in fresh and canned condition as hay, haylage, silage, meal, pellets
and paste. The aim of this study was to determine the levels of mercury in soil and plants Medicago
sativa L.. to obtain information on safety of this nutrient. The examination was conducted in Hrtkovei,
in the Republic of Serbia. Examined soil belongs to the automorphic soils, humus-accumulative class,
type of chernozem, subtypes on loess and loess-like sediments and variety gleyic chernozem, with
profile Ajno-AC-C. Soil has a good physical and chemical properties and is suitable for the production
of alfalfa, if contamination with heavy metals would not be a limiting factor. The study involved eight
localities. Sampling of soil and plant material was carried out in May 2011., in the second production
year of alfalfa. Soil samples were taken in a disrupted state with agrochemical probe, at a depth of 0-30
cm. Mercury content was determined by cold vapor technique, using atomic absorption
spectrophotometer. The mercury content in soil samples of examined localities was below the 0.001
mg/kg, which was not exceeded maximum level of 2.0 mg/kg, according to the Regulations on
permitted amounts of hazardous and harmful substances in soil and water for irrigation and methods of
their analysis (Slu%beni glasnik RS, no. 23/1994). The content of mercury in Medicago sativa L. was
below 0.001 mg/kg, which is below the maximum permitted concentration, according to the
Regulations on the quality of the feed (Sluzbeni glasnik RS, no. 4/2010) is 0.1 mg/kg. On the basis of
these results it can be concluded that the examined samples of alfalfa are suitable for feeding animals
and do not threaten the food chain, as well as safe food production.",
publisher = "National Society of Processing and Energy in Agriculture, Novi Sad, Serbia",
journal = "3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEPl, Vrnjačka Banja, 21 April 2013",
title = "The content of mercury in alfaalfa samples from gleyic chernozem",
pages = "298-297",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3722"
}

Jakšić, S., Vasin, J., Grahovac, N., Popović, V., Šunjka, D., Mijić, B.,& Komlen, V.. (2013). The content of mercury in alfaalfa samples from gleyic chernozem. in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEPl, Vrnjačka Banja, 21 April 2013
National Society of Processing and Energy in Agriculture, Novi Sad, Serbia., 297-298.
https://hdl.handle.net/21.15107/rcub_fiver_3722

Jakšić S, Vasin J, Grahovac N, Popović V, Šunjka D, Mijić B, Komlen V. The content of mercury in alfaalfa samples from gleyic chernozem. in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEPl, Vrnjačka Banja, 21 April 2013. 2013;:297-298.
https://hdl.handle.net/21.15107/rcub_fiver_3722 .

Jakšić, Snežana, Vasin, Jovica, Grahovac, Nada, Popović, Vera, Šunjka, D., Mijić, B., Komlen, V., "The content of mercury in alfaalfa samples from gleyic chernozem" in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEPl, Vrnjačka Banja, 21 April 2013 (2013):297-298,
https://hdl.handle.net/21.15107/rcub_fiver_3722 .

TY  - CONF
AU  - Lazić, S.
AU  - Grahovac, Nada
AU  - Šunjka, D.
AU  - Guzsvány, V.
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3700
AB  - Chlorinated acids are selective agricultural herbicides which are widely employed in agriculture and gardening for control the growth of different unwanted
vegetable species in crops. Because of high water solubility and toxicological risk
of some acid herbicides and their metabolic products, monitoring of their concentration in surface and groundwater is very important task. The acidic herbicides
are manufactured in formulation as free acids, as their alkaline salts or as esters.
The unionized free acids vary in water solubility (Table 1), but the acidic herbicides most frequently exist in ionized form at environmental pH values. Acidic
herbicides formulated as salts are water soluble, while those formulations prepared as esters are less water soluble. In the environment, acidic herbicides formulated as esters have short hydrolysis half-life time (24-48 h) and therefore they are
generally present as ionized acids. For most analytes, especially for the acidic herbicides, solid phase extraction (SPE) is the choice of sample treatment, which is
followed by appropriate chromatographic separation and sensitive determination
of target components. For the acidic herbicides, combination of physico-chemical
parameters influences their extraction from aqueous solution. Ionogenicity (pKa)
and hydrophobicity (logkow) are especially important in determining the approach of SPE for efficient sample clean-up for further chromatographic analysis
of chlorophenoxy acid herbicide in water samples.
C3  - Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“,  Vršac
T1  - Solid-phase extraction followed by high-performance liquid chromatography with diode array detection for screening of dicamba herbicide in water
EP  - 387
SP  - 386
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3700
ER  - 

@conference{
author = "Lazić, S. and Grahovac, Nada and Šunjka, D. and Guzsvány, V. and Jakšić, Snežana",
year = "2013",
abstract = "Chlorinated acids are selective agricultural herbicides which are widely employed in agriculture and gardening for control the growth of different unwanted
vegetable species in crops. Because of high water solubility and toxicological risk
of some acid herbicides and their metabolic products, monitoring of their concentration in surface and groundwater is very important task. The acidic herbicides
are manufactured in formulation as free acids, as their alkaline salts or as esters.
The unionized free acids vary in water solubility (Table 1), but the acidic herbicides most frequently exist in ionized form at environmental pH values. Acidic
herbicides formulated as salts are water soluble, while those formulations prepared as esters are less water soluble. In the environment, acidic herbicides formulated as esters have short hydrolysis half-life time (24-48 h) and therefore they are
generally present as ionized acids. For most analytes, especially for the acidic herbicides, solid phase extraction (SPE) is the choice of sample treatment, which is
followed by appropriate chromatographic separation and sensitive determination
of target components. For the acidic herbicides, combination of physico-chemical
parameters influences their extraction from aqueous solution. Ionogenicity (pKa)
and hydrophobicity (logkow) are especially important in determining the approach of SPE for efficient sample clean-up for further chromatographic analysis
of chlorophenoxy acid herbicide in water samples.",
journal = "Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“,  Vršac",
title = "Solid-phase extraction followed by high-performance liquid chromatography with diode array detection for screening of dicamba herbicide in water",
pages = "387-386",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3700"
}

Lazić, S., Grahovac, N., Šunjka, D., Guzsvány, V.,& Jakšić, S.. (2013). Solid-phase extraction followed by high-performance liquid chromatography with diode array detection for screening of dicamba herbicide in water. in Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“,  Vršac, 386-387.
https://hdl.handle.net/21.15107/rcub_fiver_3700

Lazić S, Grahovac N, Šunjka D, Guzsvány V, Jakšić S. Solid-phase extraction followed by high-performance liquid chromatography with diode array detection for screening of dicamba herbicide in water. in Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“,  Vršac. 2013;:386-387.
https://hdl.handle.net/21.15107/rcub_fiver_3700 .

Lazić, S., Grahovac, Nada, Šunjka, D., Guzsvány, V., Jakšić, Snežana, "Solid-phase extraction followed by high-performance liquid chromatography with diode array detection for screening of dicamba herbicide in water" in Knjiga izvoda, 6. Simpozijum „Hemija i zaštita životne sredine EnviroChem 2013“,  Vršac (2013):386-387,
https://hdl.handle.net/21.15107/rcub_fiver_3700 .

TY  - CONF
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Milovanović, I
AU  - Grahovac, Nada
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3724
AB  - Non-point-source contamination of surface water has emerged as an important environmental problem during the last decades. This is particularly obvious in areas with intensive pesticide application. Considering importance of water and soil quality, pesticide residues in our environment need to be more effectively documented. This study has focused on determination of pesticide residues in drainage water. Monitoring of pesticide residues were conducted at different locations in the northern part of Serbia, the main agricultural area of the country. The sampling was performed during spring and summer 2012 on twelve potential risk sites. The extraction of pesticides from water was carried out with ENVI™ C18 DSK 47 mm (Supelco 5-7171, Bellafonte, PA). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of ultrapure water. Afterward, water sample was filtered through the disc under vacuum and disc was dried for 1 h at 25 °C. Pesticides were eluted from the disc with 5 ml of mixture dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was reconstituted in 1 ml of methanol, ultrasonically homogenized and analyzed. Analyses were performed on Agilent Technologies GC-MS Model 7890 A Series gas chromatograph coupled to 5975 C mass selective detector. A HP5MS (30 mx 0.25 mmi.d.) fused silica capillary column was used. Helium was used as the carrier gas at a constant pressure. Injection volume was 2.0 ul, in splitless mode at 280 °C. The total run time was 41.86 min. The interface was kept at 250 °C, the quadropole at 150 °C and the mass spectra were obtained at electron energy of 70 eV. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of pesticide residues in water samples. In analyzed samples 22 pesticides were detected -8fungicides,9 herbicides and 5 insecticides. Herbicides were most frequently detected (in 41% of the samples), followed by fungicides (36%) and insecticides (23%)
C3  - 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013
T1  - Screening of pesticide residues in canal water in the Northern Serbia
EP  - 134
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3724
ER  - 

@conference{
author = "Lazić, S. and Šunjka, D. and Milovanović, I and Grahovac, Nada",
year = "2013",
abstract = "Non-point-source contamination of surface water has emerged as an important environmental problem during the last decades. This is particularly obvious in areas with intensive pesticide application. Considering importance of water and soil quality, pesticide residues in our environment need to be more effectively documented. This study has focused on determination of pesticide residues in drainage water. Monitoring of pesticide residues were conducted at different locations in the northern part of Serbia, the main agricultural area of the country. The sampling was performed during spring and summer 2012 on twelve potential risk sites. The extraction of pesticides from water was carried out with ENVI™ C18 DSK 47 mm (Supelco 5-7171, Bellafonte, PA). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of ultrapure water. Afterward, water sample was filtered through the disc under vacuum and disc was dried for 1 h at 25 °C. Pesticides were eluted from the disc with 5 ml of mixture dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was reconstituted in 1 ml of methanol, ultrasonically homogenized and analyzed. Analyses were performed on Agilent Technologies GC-MS Model 7890 A Series gas chromatograph coupled to 5975 C mass selective detector. A HP5MS (30 mx 0.25 mmi.d.) fused silica capillary column was used. Helium was used as the carrier gas at a constant pressure. Injection volume was 2.0 ul, in splitless mode at 280 °C. The total run time was 41.86 min. The interface was kept at 250 °C, the quadropole at 150 °C and the mass spectra were obtained at electron energy of 70 eV. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of pesticide residues in water samples. In analyzed samples 22 pesticides were detected -8fungicides,9 herbicides and 5 insecticides. Herbicides were most frequently detected (in 41% of the samples), followed by fungicides (36%) and insecticides (23%)",
journal = "14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013",
title = "Screening of pesticide residues in canal water in the Northern Serbia",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3724"
}

Lazić, S., Šunjka, D., Milovanović, I.,& Grahovac, N.. (2013). Screening of pesticide residues in canal water in the Northern Serbia. in 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013, 134-134.
https://hdl.handle.net/21.15107/rcub_fiver_3724

Lazić S, Šunjka D, Milovanović I, Grahovac N. Screening of pesticide residues in canal water in the Northern Serbia. in 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013. 2013;:134-134.
https://hdl.handle.net/21.15107/rcub_fiver_3724 .

Lazić, S., Šunjka, D., Milovanović, I, Grahovac, Nada, "Screening of pesticide residues in canal water in the Northern Serbia" in 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013 (2013):134-134,
https://hdl.handle.net/21.15107/rcub_fiver_3724 .

TY  - CONF
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Grahovac, Nada
AU  - Guzsvány, V.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3732
AB  - The characteristics of water used in a spray mix influence the
effectiveness of pesticides. It is important to know the pH of water used
with a pesticide and the susceptibility of the pesticide to hydrolysis.
In order to investigate the persistence, dissipation and degradation
kinetics of bentazone and dicamba, laboratory study was conducted
in deionized water (pH 7.0) at 2542°C (T1) and 442°C (T2). Herbicides
were dissolved at rate of 0.05 mg/ml. Concentration of analytes was
monitoried 0 (1h), 2, 4, 7, 10, 14, 18, 24 and 28 days after treatment and
analysing as triplicate samples. Samples were analyzed by high pressure
liquid chromatography (HPLC/DAD). The chromatographic separation
was carried out with Zorbax Eclipse XDB-C,, (50 mm x 4.6 mm x 1.8 pm)
analytical column, using reverse phase column with gradient conditions
of mobile phase consist of water (with 0.05% H PO,) and acetonitrile. In
T1 the dissipation were 1.3, 5.1, 7.5, 7.9, 8.1, 15.3, 24.1, 26.9% for dicamba
and 11.5, 33.3, 44.6, 46.9,48.8, 49.6, 50.1, 51.8% for bentazone in 2, 4, 7,
10, 14, 18, 24, and 28 days. Corresponding dissipation in T2 experiment
were 2.0, 2.7, 3.9, 5.0, 6.1, 6.9, 6.8, 8.0% for dicamba and 7.7, 15.2, 26.7;
29.4, 30.3, 30.6, 31.4, 31.9% for bentazone, respectively. The dissipation
data in water showed the DT, and DT,, values 57.3 and 114.9 days
ae for dicamba and 17.1 and 125.9 days for bentazone herbicide. Several
simulation models were used to evaluate the experimental data, such
as Exponential and Mittag-Leffler function. The dissipation of analized
eo . herbicides residues over the time in water were described by the MittagLeffler function, with the best-fit model for dicamba and bentazone [1].
The dissipation of dicamba and bentazone residues on 25+2°C and 442°C
es over the time in deinoized water were described by function a*Eo, (-bt).
ag Coefficients a, b, a, 8 were obtained from the experimental data by using
be fitting procedure. We got for dicamba and bentazone herbicides on
a 25+2°C coefficients a=0.8, R=3.71,a=159.11,b=12.79 and a=2.17, B=4.56,
Es a=1387.45, b=1.96 for 442 °C a=0.8, B=4.11, a=82.53, b=7.0 and a=0.99,
rs G=3.15, a=205.81, b=0.117, respectively. The hydrolysis study indicated 
that the dicamba and bentazone pesticides hydrolysed faster at 25+2°C.
These findings can be useful in the prediction of the dissipation behavior
of this pesticides in the spray tank immediately before application. The
dissipation rates of dicamba and bentazone pesticides depended on the
temperature and pH of water to be used.
C3  - 14th European Meeting on Enviromental Chemistry, Budva, Montenegro
T1  - Persistence and dissipation behavior of dicamba and bentazon herbicides in water under laboratory conditions
EP  - 86
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3732
ER  - 

@conference{
author = "Lazić, S. and Šunjka, D. and Grahovac, Nada and Guzsvány, V.",
year = "2013",
abstract = "The characteristics of water used in a spray mix influence the
effectiveness of pesticides. It is important to know the pH of water used
with a pesticide and the susceptibility of the pesticide to hydrolysis.
In order to investigate the persistence, dissipation and degradation
kinetics of bentazone and dicamba, laboratory study was conducted
in deionized water (pH 7.0) at 2542°C (T1) and 442°C (T2). Herbicides
were dissolved at rate of 0.05 mg/ml. Concentration of analytes was
monitoried 0 (1h), 2, 4, 7, 10, 14, 18, 24 and 28 days after treatment and
analysing as triplicate samples. Samples were analyzed by high pressure
liquid chromatography (HPLC/DAD). The chromatographic separation
was carried out with Zorbax Eclipse XDB-C,, (50 mm x 4.6 mm x 1.8 pm)
analytical column, using reverse phase column with gradient conditions
of mobile phase consist of water (with 0.05% H PO,) and acetonitrile. In
T1 the dissipation were 1.3, 5.1, 7.5, 7.9, 8.1, 15.3, 24.1, 26.9% for dicamba
and 11.5, 33.3, 44.6, 46.9,48.8, 49.6, 50.1, 51.8% for bentazone in 2, 4, 7,
10, 14, 18, 24, and 28 days. Corresponding dissipation in T2 experiment
were 2.0, 2.7, 3.9, 5.0, 6.1, 6.9, 6.8, 8.0% for dicamba and 7.7, 15.2, 26.7;
29.4, 30.3, 30.6, 31.4, 31.9% for bentazone, respectively. The dissipation
data in water showed the DT, and DT,, values 57.3 and 114.9 days
ae for dicamba and 17.1 and 125.9 days for bentazone herbicide. Several
simulation models were used to evaluate the experimental data, such
as Exponential and Mittag-Leffler function. The dissipation of analized
eo . herbicides residues over the time in water were described by the MittagLeffler function, with the best-fit model for dicamba and bentazone [1].
The dissipation of dicamba and bentazone residues on 25+2°C and 442°C
es over the time in deinoized water were described by function a*Eo, (-bt).
ag Coefficients a, b, a, 8 were obtained from the experimental data by using
be fitting procedure. We got for dicamba and bentazone herbicides on
a 25+2°C coefficients a=0.8, R=3.71,a=159.11,b=12.79 and a=2.17, B=4.56,
Es a=1387.45, b=1.96 for 442 °C a=0.8, B=4.11, a=82.53, b=7.0 and a=0.99,
rs G=3.15, a=205.81, b=0.117, respectively. The hydrolysis study indicated 
that the dicamba and bentazone pesticides hydrolysed faster at 25+2°C.
These findings can be useful in the prediction of the dissipation behavior
of this pesticides in the spray tank immediately before application. The
dissipation rates of dicamba and bentazone pesticides depended on the
temperature and pH of water to be used.",
journal = "14th European Meeting on Enviromental Chemistry, Budva, Montenegro",
title = "Persistence and dissipation behavior of dicamba and bentazon herbicides in water under laboratory conditions",
pages = "86-86",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3732"
}

Lazić, S., Šunjka, D., Grahovac, N.,& Guzsvány, V.. (2013). Persistence and dissipation behavior of dicamba and bentazon herbicides in water under laboratory conditions. in 14th European Meeting on Enviromental Chemistry, Budva, Montenegro, 86-86.
https://hdl.handle.net/21.15107/rcub_fiver_3732

Lazić S, Šunjka D, Grahovac N, Guzsvány V. Persistence and dissipation behavior of dicamba and bentazon herbicides in water under laboratory conditions. in 14th European Meeting on Enviromental Chemistry, Budva, Montenegro. 2013;:86-86.
https://hdl.handle.net/21.15107/rcub_fiver_3732 .

Lazić, S., Šunjka, D., Grahovac, Nada, Guzsvány, V., "Persistence and dissipation behavior of dicamba and bentazon herbicides in water under laboratory conditions" in 14th European Meeting on Enviromental Chemistry, Budva, Montenegro (2013):86-86,
https://hdl.handle.net/21.15107/rcub_fiver_3732 .

TY  - CONF
AU  - Jakšić, Snežana
AU  - Vasiljević, Sanja
AU  - Vasin, Jovica
AU  - Marinković, Jelena
AU  - Komlen, V.
AU  - Grahovac, Nada
AU  - Šunjka, D.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3729
AB  - Mikroorganizmi su indikatori plodnosti zemljišta, ali i procesa njegove degradacije. Povećane količine teških metala u zemljištu ili u zemljišnom rastvoru mogu imati toksičan efekat na rast i razviće svih sistematskih i fizioloških grupa mikroorganizama u zemljištu. Cilj ovoga rada je bio da se utvrde nivoi olova i hroma u fluvisolu. te njihov uticaj na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L. Ukupan sadržaj Pb i Cru ispitivanim uzorcima ackarbonatnog fluvisola bio je iznad maksimalno dozvoljene količine, što znači da svi uzorci analiziranih lokaliteta pripadaju kategoriji kontaminiranog zemljišta. Konstatovana je značajna negativna korelaciona zavisnost između sadržaja olova i brojnosti amonifikatora. Ukupan broj mikroorganizama i aktivnost dehidrogenaze nisu se menjali pod uticajem povećanog sadržaja olova. Povećanjem sadrzaja hroma ukupan broj mikroorganizama i aktivnost dehidrogenaze su se smanjili, dok je broj amonifikatora bio u slaboj zavisnosti od sadržaja hroma.
AB  - Microorganisms are indicators of soil fertility and soil degradation. Increased amounts of heavy metals in soils and in the soil solution can have a toxic effect on the growth and development of systematic and physiological groups of microorganisms in the soil. The aim of this study was to determine the levels of lead and chromitim in fluvisol and its effect on the microbial activity in the soil under Trifolium pratense L. The total content of Pb and Cr in the samples of non-carbonate fluvisol was above the maximum allowable amount. which means that all samples analyzed sites belong to the category of contaminated soil. We found a significant negative correlation between lead content and the number of ammonitiors. The total number of microorganisms and dehydrogenase activity are not changed under the influence of increased lead content. Increasing chromium content of total microorganisms and dehydrogenase activity were decreased. while the number ammonifiers was in a weak dependence of the chromium content.
C3  - Abstract Proceedings, International Conference Submediterranean Agriculture-ICSA 2012, Mostar, 22-24 November 2012
T1  - Uticaj olova i hroma na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L.
T1  - Effect of lead and chromium on microbial activity of soil under Trifolium pratense L.
EP  - 34
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3729
ER  - 

@conference{
author = "Jakšić, Snežana and Vasiljević, Sanja and Vasin, Jovica and Marinković, Jelena and Komlen, V. and Grahovac, Nada and Šunjka, D.",
year = "2012",
abstract = "Mikroorganizmi su indikatori plodnosti zemljišta, ali i procesa njegove degradacije. Povećane količine teških metala u zemljištu ili u zemljišnom rastvoru mogu imati toksičan efekat na rast i razviće svih sistematskih i fizioloških grupa mikroorganizama u zemljištu. Cilj ovoga rada je bio da se utvrde nivoi olova i hroma u fluvisolu. te njihov uticaj na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L. Ukupan sadržaj Pb i Cru ispitivanim uzorcima ackarbonatnog fluvisola bio je iznad maksimalno dozvoljene količine, što znači da svi uzorci analiziranih lokaliteta pripadaju kategoriji kontaminiranog zemljišta. Konstatovana je značajna negativna korelaciona zavisnost između sadržaja olova i brojnosti amonifikatora. Ukupan broj mikroorganizama i aktivnost dehidrogenaze nisu se menjali pod uticajem povećanog sadržaja olova. Povećanjem sadrzaja hroma ukupan broj mikroorganizama i aktivnost dehidrogenaze su se smanjili, dok je broj amonifikatora bio u slaboj zavisnosti od sadržaja hroma., Microorganisms are indicators of soil fertility and soil degradation. Increased amounts of heavy metals in soils and in the soil solution can have a toxic effect on the growth and development of systematic and physiological groups of microorganisms in the soil. The aim of this study was to determine the levels of lead and chromitim in fluvisol and its effect on the microbial activity in the soil under Trifolium pratense L. The total content of Pb and Cr in the samples of non-carbonate fluvisol was above the maximum allowable amount. which means that all samples analyzed sites belong to the category of contaminated soil. We found a significant negative correlation between lead content and the number of ammonitiors. The total number of microorganisms and dehydrogenase activity are not changed under the influence of increased lead content. Increasing chromium content of total microorganisms and dehydrogenase activity were decreased. while the number ammonifiers was in a weak dependence of the chromium content.",
journal = "Abstract Proceedings, International Conference Submediterranean Agriculture-ICSA 2012, Mostar, 22-24 November 2012",
title = "Uticaj olova i hroma na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L., Effect of lead and chromium on microbial activity of soil under Trifolium pratense L.",
pages = "34-34",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3729"
}

Jakšić, S., Vasiljević, S., Vasin, J., Marinković, J., Komlen, V., Grahovac, N.,& Šunjka, D.. (2012). Uticaj olova i hroma na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L.. in Abstract Proceedings, International Conference Submediterranean Agriculture-ICSA 2012, Mostar, 22-24 November 2012, 34-34.
https://hdl.handle.net/21.15107/rcub_fiver_3729

Jakšić S, Vasiljević S, Vasin J, Marinković J, Komlen V, Grahovac N, Šunjka D. Uticaj olova i hroma na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L.. in Abstract Proceedings, International Conference Submediterranean Agriculture-ICSA 2012, Mostar, 22-24 November 2012. 2012;:34-34.
https://hdl.handle.net/21.15107/rcub_fiver_3729 .

Jakšić, Snežana, Vasiljević, Sanja, Vasin, Jovica, Marinković, Jelena, Komlen, V., Grahovac, Nada, Šunjka, D., "Uticaj olova i hroma na mikrobiološku aktivnost u zemljištu pod usevom Trifolium pratense L." in Abstract Proceedings, International Conference Submediterranean Agriculture-ICSA 2012, Mostar, 22-24 November 2012 (2012):34-34,
https://hdl.handle.net/21.15107/rcub_fiver_3729 .

TY  - CONF
AU  - Lazić, S.
AU  - Grahovac, Nada
AU  - Šunjka, D.
AU  - Pucarevic, M.
AU  - Jakšić, Snežana
AU  - Popović, Vera
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3726
AB  - Chlorpyrifos is one of organophosphorus (OPPs) insecticides, which are the most important and widely used classes of agricultural pesticides. Because of its intensive use of ecosystems are contaminated by chlorpyrifos and public concerns on safety have been increased.
The method has been developed for confirmation and quantitation of the chlorpyrifos in water samples reverse phase high performance liquid chromatography (HPLC) with photodiodes detection and chromatograms were extracted at 300 nm. This method was single-lab validate in order to demonstrate its performance for monitoring of the insecticide chlorpyrifos in water.
Method accuracy was quantified through measurement uncertainty estimate based on method validation data. Tap and groundwater samples were used to demonstrate linearity and accuracy. Linearity for chlorpyrifos was evaluated by calibration curves in the range of 0.01—10 pg/ml. The accuracy of the proposed method was evaluated as the mean recovery. The analytical method allowed us to quantify chlorpyrifos in tap water and groundwater in concentration level between 0.01-—10 pg/ml with recovery values between of 80.72-109.57 % for tap water and 89.99-97.45 % for groundwater and repeatability lower or equal than 0.6% for both the matrices. The experiments showed that there were no interference peaks from the water matrix (tap and ground) on the elution region of the chlorpyrifos. The RSD values were satisfactory being in the ranges of 0.283-1.237 % (for tap water) and 0.681-3.887 % (for groundwater). The variation of the retention time was less than 0.05 % during these experimental series. The combined relative uncertainty for water samples was 0.481 % (for tap water) and 0.654 % (for groundwater). The expanded uncertainty (Uc) was calculated as Uc= k*u,, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEMICITAC, 2000).
We proved that the method was specific for determination of chlorpyrifos in the relevant matrices. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of chlorpyrifos in water samples.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012
T1  - Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis
EP  - 73
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3726
ER  - 

@conference{
author = "Lazić, S. and Grahovac, Nada and Šunjka, D. and Pucarevic, M. and Jakšić, Snežana and Popović, Vera",
year = "2012",
abstract = "Chlorpyrifos is one of organophosphorus (OPPs) insecticides, which are the most important and widely used classes of agricultural pesticides. Because of its intensive use of ecosystems are contaminated by chlorpyrifos and public concerns on safety have been increased.
The method has been developed for confirmation and quantitation of the chlorpyrifos in water samples reverse phase high performance liquid chromatography (HPLC) with photodiodes detection and chromatograms were extracted at 300 nm. This method was single-lab validate in order to demonstrate its performance for monitoring of the insecticide chlorpyrifos in water.
Method accuracy was quantified through measurement uncertainty estimate based on method validation data. Tap and groundwater samples were used to demonstrate linearity and accuracy. Linearity for chlorpyrifos was evaluated by calibration curves in the range of 0.01—10 pg/ml. The accuracy of the proposed method was evaluated as the mean recovery. The analytical method allowed us to quantify chlorpyrifos in tap water and groundwater in concentration level between 0.01-—10 pg/ml with recovery values between of 80.72-109.57 % for tap water and 89.99-97.45 % for groundwater and repeatability lower or equal than 0.6% for both the matrices. The experiments showed that there were no interference peaks from the water matrix (tap and ground) on the elution region of the chlorpyrifos. The RSD values were satisfactory being in the ranges of 0.283-1.237 % (for tap water) and 0.681-3.887 % (for groundwater). The variation of the retention time was less than 0.05 % during these experimental series. The combined relative uncertainty for water samples was 0.481 % (for tap water) and 0.654 % (for groundwater). The expanded uncertainty (Uc) was calculated as Uc= k*u,, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEMICITAC, 2000).
We proved that the method was specific for determination of chlorpyrifos in the relevant matrices. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of chlorpyrifos in water samples.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012",
title = "Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3726"
}

Lazić, S., Grahovac, N., Šunjka, D., Pucarevic, M., Jakšić, S.,& Popović, V.. (2012). Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis. in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012
Beograd : Društvo za zaštitu bilja Srbije., 73-73.
https://hdl.handle.net/21.15107/rcub_fiver_3726

Lazić S, Grahovac N, Šunjka D, Pucarevic M, Jakšić S, Popović V. Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis. in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012. 2012;:73-73.
https://hdl.handle.net/21.15107/rcub_fiver_3726 .

Lazić, S., Grahovac, Nada, Šunjka, D., Pucarevic, M., Jakšić, Snežana, Popović, Vera, "Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis" in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012 (2012):73-73,
https://hdl.handle.net/21.15107/rcub_fiver_3726 .

TY  - CONF
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Grahovac, Nada
AU  - Komlen, V.
AU  - Bagi, F
AU  - Budakov, D.
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3730
AB  - U radu je razvijena brza i jednostavna metoda za istovremeno određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline (6HNK) u uzorcima trešanja. Metoda se bazira na primeni reverzne faze razdvajanja na C18 koloni primenom gradijentne elucije. Za određivanje i kvantifikaciju analita primenjena je tečna hromatografija sa detektorom sa nizom diada (HPLC/DAD), pri talasnoj dužini od 230 nm. Ekstrakcija acetamiprida 6HNK izvedena je mešavinom acetonitril/0.IN NHyCl. Prosečne vrednosti prinosa ekstrakcije aceiamiprida 6HNK u uzorcima trešanja kretale su se u intervalima od 95-101% 1 73-83% (RSD<5%), respektivno,
AB  - A rapid and simple method for the simultaneous analysis and quantification of acetamiprid and 6-chloronicotinie acid (6CNA). as intermediate of acetamiprid decomposition in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C8 column with gradient elution. Analytes’ determination and quantification were performed by HPLC/DAD and chromatograms were extracted at 230 nm. These insecticides were extracted with mixture acetonitril/0.1N NH)CL Average recoveries of acekumiprid and 6CNA from sweet cherry samples were in range between 95-101 % and 73- $3%, respectively. with RSDs<5%.
C3  - International Conference Submediterranean Agriculture-ICSA, Mostar, 22 November 2012
T1  - Mogućnost određivanja rezidua acetamiprida i njegovog metabolita u trešnjama primenom HPLC/DAD
T1  - Possibility for determination of acetamiprid and its metabolite residues in sweet cherry using HPLC/DAD
EP  - 18
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3730
ER  - 

@conference{
author = "Lazić, S. and Šunjka, D. and Grahovac, Nada and Komlen, V. and Bagi, F and Budakov, D. and Jakšić, Snežana",
year = "2012",
abstract = "U radu je razvijena brza i jednostavna metoda za istovremeno određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline (6HNK) u uzorcima trešanja. Metoda se bazira na primeni reverzne faze razdvajanja na C18 koloni primenom gradijentne elucije. Za određivanje i kvantifikaciju analita primenjena je tečna hromatografija sa detektorom sa nizom diada (HPLC/DAD), pri talasnoj dužini od 230 nm. Ekstrakcija acetamiprida 6HNK izvedena je mešavinom acetonitril/0.IN NHyCl. Prosečne vrednosti prinosa ekstrakcije aceiamiprida 6HNK u uzorcima trešanja kretale su se u intervalima od 95-101% 1 73-83% (RSD<5%), respektivno,, A rapid and simple method for the simultaneous analysis and quantification of acetamiprid and 6-chloronicotinie acid (6CNA). as intermediate of acetamiprid decomposition in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C8 column with gradient elution. Analytes’ determination and quantification were performed by HPLC/DAD and chromatograms were extracted at 230 nm. These insecticides were extracted with mixture acetonitril/0.1N NH)CL Average recoveries of acekumiprid and 6CNA from sweet cherry samples were in range between 95-101 % and 73- $3%, respectively. with RSDs<5%.",
journal = "International Conference Submediterranean Agriculture-ICSA, Mostar, 22 November 2012",
title = "Mogućnost određivanja rezidua acetamiprida i njegovog metabolita u trešnjama primenom HPLC/DAD, Possibility for determination of acetamiprid and its metabolite residues in sweet cherry using HPLC/DAD",
pages = "18-18",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3730"
}

Lazić, S., Šunjka, D., Grahovac, N., Komlen, V., Bagi, F., Budakov, D.,& Jakšić, S.. (2012). Mogućnost određivanja rezidua acetamiprida i njegovog metabolita u trešnjama primenom HPLC/DAD. in International Conference Submediterranean Agriculture-ICSA, Mostar, 22 November 2012, 18-18.
https://hdl.handle.net/21.15107/rcub_fiver_3730

Lazić S, Šunjka D, Grahovac N, Komlen V, Bagi F, Budakov D, Jakšić S. Mogućnost određivanja rezidua acetamiprida i njegovog metabolita u trešnjama primenom HPLC/DAD. in International Conference Submediterranean Agriculture-ICSA, Mostar, 22 November 2012. 2012;:18-18.
https://hdl.handle.net/21.15107/rcub_fiver_3730 .

Lazić, S., Šunjka, D., Grahovac, Nada, Komlen, V., Bagi, F, Budakov, D., Jakšić, Snežana, "Mogućnost određivanja rezidua acetamiprida i njegovog metabolita u trešnjama primenom HPLC/DAD" in International Conference Submediterranean Agriculture-ICSA, Mostar, 22 November 2012 (2012):18-18,
https://hdl.handle.net/21.15107/rcub_fiver_3730 .

TY  - CONF
AU  - Lazić, S.
AU  - Grahovac, Nada
AU  - Šunjka, D.
AU  - Jakšić, Snežana
AU  - Ljubojević, M.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3709
AB  - Pojava ostataka pesticida u voću i povrću moguća je i pored poštovanja principa dobre poljoprivredne prakse. Identifikacija prisutnih pesticida i određivanje njihovih količina u ovim matriksima zahteva primenu metoda visoke osetljivosti i selektivnosti. Istraživanje je sprovedeno u cilju iznalaženja optimalnih eksperimentalnih uslova za razdvajanje i određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline (6-HNK) u uzorcima trešanja, korišćenjem tečne hromatografije sa nizom fotoosetljivin dioda (DAD). Uzorci trešanja su korišćeni da bi se odredila selektivnost, linearnost, tačnost i limit kvantifikacije (LOQ). Tačnost metode je kvantifikovana kroz procenu merne neizvesnosti zasnovanu na podacima validacije metode.
PB  - Društvo za zaštitu bilja Srbije 11080 Beograd 80, Nemanjina 6; p.fah 123
C3  - Zbornik rezimea radova, 12. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30. novembar 2012.
T1  - Procena merne neizvesnosti i validacija metode određivanja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja
EP  - 81
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3709
ER  - 

@conference{
author = "Lazić, S. and Grahovac, Nada and Šunjka, D. and Jakšić, Snežana and Ljubojević, M.",
year = "2012",
abstract = "Pojava ostataka pesticida u voću i povrću moguća je i pored poštovanja principa dobre poljoprivredne prakse. Identifikacija prisutnih pesticida i određivanje njihovih količina u ovim matriksima zahteva primenu metoda visoke osetljivosti i selektivnosti. Istraživanje je sprovedeno u cilju iznalaženja optimalnih eksperimentalnih uslova za razdvajanje i određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline (6-HNK) u uzorcima trešanja, korišćenjem tečne hromatografije sa nizom fotoosetljivin dioda (DAD). Uzorci trešanja su korišćeni da bi se odredila selektivnost, linearnost, tačnost i limit kvantifikacije (LOQ). Tačnost metode je kvantifikovana kroz procenu merne neizvesnosti zasnovanu na podacima validacije metode.",
publisher = "Društvo za zaštitu bilja Srbije 11080 Beograd 80, Nemanjina 6; p.fah 123",
journal = "Zbornik rezimea radova, 12. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30. novembar 2012.",
title = "Procena merne neizvesnosti i validacija metode određivanja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja",
pages = "81-80",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3709"
}

Lazić, S., Grahovac, N., Šunjka, D., Jakšić, S.,& Ljubojević, M.. (2012). Procena merne neizvesnosti i validacija metode određivanja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja. in Zbornik rezimea radova, 12. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30. novembar 2012.
Društvo za zaštitu bilja Srbije 11080 Beograd 80, Nemanjina 6; p.fah 123., 80-81.
https://hdl.handle.net/21.15107/rcub_fiver_3709

Lazić S, Grahovac N, Šunjka D, Jakšić S, Ljubojević M. Procena merne neizvesnosti i validacija metode određivanja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja. in Zbornik rezimea radova, 12. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30. novembar 2012.. 2012;:80-81.
https://hdl.handle.net/21.15107/rcub_fiver_3709 .

Lazić, S., Grahovac, Nada, Šunjka, D., Jakšić, Snežana, Ljubojević, M., "Procena merne neizvesnosti i validacija metode određivanja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja" in Zbornik rezimea radova, 12. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30. novembar 2012. (2012):80-81,
https://hdl.handle.net/21.15107/rcub_fiver_3709 .

TY  - CONF
AU  - Šunjka, D.
AU  - Lazić, S.
AU  - Grahovac, Nada
AU  - Ljubojević, M.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3727
AB  - Acetamiprid is a broad-spectrum neonicotinoids insecticide used on a wide range crops, especially fruits. Besides its positive effects, acetamiprid also has been posing various health risks to consumers. Due to the growing use of insecticides from the family of neonicotinoids, their increased presence in the environment is evident. For this reason, the concentration of acetamiprid residues, including its metabolite 6-chloronicotinic acid, in agricultural products should be monitored.
A rapid and simple method for the confirmation, simultaneous analysis and quantification of acetamiprid and 6-chloronicotinic acid as intermediate of acetamiprid decomposition in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes’ determination and quantification were performed by high performance _ liquid chromatography (HPLC) with photodiodes detection and chromatograms were extracted at 230nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with mixture acetonitril/0.1N ammonium-chloride (8/2, v/v). The extract was filtered through layer of celit and evaporated, the residue dissolved with acetone and then analyzed by liquid chromatography. The standard addition method was used for acetamiprid and 6-chloronicotinic acid determination in order to eliminate the matrix effect. Sweet cherry samples spiked with the concentration levels of 0.5mg/kg and 1mg/kg was used to ensure method accuracy (recovery) and data precision. The repeatability of the retention times and peak areas were checked by injecting the standard mixture of acetamiprid and 6-chloronicotinic acid solution five times. Average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively with the associated relative standard deviations (RSDs) < 5%. Limit of detection (LOD) for the analyzed acetamiprid and 6-chloronicotinic acid was estimated from fortified samples. The limit of quantification (LOQ) was 10 and 30u9/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, it can be concluded that the developed HPLC-DAD method represents a useful tool for a sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012
T1  - Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples
EP  - 65
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3727
ER  - 

@conference{
author = "Šunjka, D. and Lazić, S. and Grahovac, Nada and Ljubojević, M.",
year = "2012",
abstract = "Acetamiprid is a broad-spectrum neonicotinoids insecticide used on a wide range crops, especially fruits. Besides its positive effects, acetamiprid also has been posing various health risks to consumers. Due to the growing use of insecticides from the family of neonicotinoids, their increased presence in the environment is evident. For this reason, the concentration of acetamiprid residues, including its metabolite 6-chloronicotinic acid, in agricultural products should be monitored.
A rapid and simple method for the confirmation, simultaneous analysis and quantification of acetamiprid and 6-chloronicotinic acid as intermediate of acetamiprid decomposition in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes’ determination and quantification were performed by high performance _ liquid chromatography (HPLC) with photodiodes detection and chromatograms were extracted at 230nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with mixture acetonitril/0.1N ammonium-chloride (8/2, v/v). The extract was filtered through layer of celit and evaporated, the residue dissolved with acetone and then analyzed by liquid chromatography. The standard addition method was used for acetamiprid and 6-chloronicotinic acid determination in order to eliminate the matrix effect. Sweet cherry samples spiked with the concentration levels of 0.5mg/kg and 1mg/kg was used to ensure method accuracy (recovery) and data precision. The repeatability of the retention times and peak areas were checked by injecting the standard mixture of acetamiprid and 6-chloronicotinic acid solution five times. Average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively with the associated relative standard deviations (RSDs) < 5%. Limit of detection (LOD) for the analyzed acetamiprid and 6-chloronicotinic acid was estimated from fortified samples. The limit of quantification (LOQ) was 10 and 30u9/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, it can be concluded that the developed HPLC-DAD method represents a useful tool for a sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012",
title = "Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3727"
}

Šunjka, D., Lazić, S., Grahovac, N.,& Ljubojević, M.. (2012). Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples. in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012
Beograd : Društvo za zaštitu bilja Srbije., 65-65.
https://hdl.handle.net/21.15107/rcub_fiver_3727

Šunjka D, Lazić S, Grahovac N, Ljubojević M. Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples. in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012. 2012;:65-65.
https://hdl.handle.net/21.15107/rcub_fiver_3727 .

Šunjka, D., Lazić, S., Grahovac, Nada, Ljubojević, M., "Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples" in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012 (2012):65-65,
https://hdl.handle.net/21.15107/rcub_fiver_3727 .

TY  - JOUR
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Grahovac, Nada
AU  - Vuković, S.
AU  - Jakšić, Snežana
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/3718
AB  - The quality of the water for irrigation in conventional, but especially in
organic food production is very important. The presence of pesticide residues in
water may cause yield reduction and decrease product quality, due to its uptake.
Chlorpyrifos is one of the most widely used insecticide in the world and it ts
detected in surface and groundwaters more frequently than any other
organophosphorus insecticide. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
The aim of this study was determination of chlorpyrifos in river and
ground water, wich are widely used for irrigation in agricultural production. The
Extraction of chlorpyrifos was performed using ENVI C18 SP disc (47 mm).
Prior to the extraction disc was conditioned with 5 ml of acetonitrile/methanol
(50/50, v/v) and 5 ml deionized water. Under vacuum 500 ml of river and ground
water with added chlorpyrifos standard solutions in concentration 0.01-1.0 ug ml
', was filtered through the disc. Disc was being dried at 25°C for I h.
Chlorpyrifos was eluted with 6 ml (23 ml) of acetonitrile/methanol (50/50, v/v)
mixture and evaporated to dryness. The extract was diluted in 2 ml of
acetonitrile/methanol (50/50, v/v). Analysis was performed with a Hewlett—
Packard (HP) model 5890 Series II gas chromatograph with EC Ni®’ detector.
Determination conditions were — t °C of column 190 °C with an increase of 30°C
min’! up to 275°C, t°C of injector 230°C and t°C of detector 300°C. The linearity
of the method was evaluated by chromatographing chlorpyrifos solution in
concentrations 0.001-1.0 pg mI'. Correlation coefficient (R°) was 0.995 and limit
of detection (LOD) and limit of quantification (LOQ) were 0.004 pg ml! and
0.01 wg mI', respectively. The average recovery for river water was 87.5% and
for groundwater 97.9%.
T2  - Poljoprivreda i šumarstvo
T1  - Determination of chlorpyrifos in water used for agricultural production
EP  - 25
IS  - 4
SP  - 17
VL  - 57
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3718
ER  - 

@article{
author = "Lazić, S. and Šunjka, D. and Grahovac, Nada and Vuković, S. and Jakšić, Snežana",
year = "2011",
abstract = "The quality of the water for irrigation in conventional, but especially in
organic food production is very important. The presence of pesticide residues in
water may cause yield reduction and decrease product quality, due to its uptake.
Chlorpyrifos is one of the most widely used insecticide in the world and it ts
detected in surface and groundwaters more frequently than any other
organophosphorus insecticide. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
The aim of this study was determination of chlorpyrifos in river and
ground water, wich are widely used for irrigation in agricultural production. The
Extraction of chlorpyrifos was performed using ENVI C18 SP disc (47 mm).
Prior to the extraction disc was conditioned with 5 ml of acetonitrile/methanol
(50/50, v/v) and 5 ml deionized water. Under vacuum 500 ml of river and ground
water with added chlorpyrifos standard solutions in concentration 0.01-1.0 ug ml
', was filtered through the disc. Disc was being dried at 25°C for I h.
Chlorpyrifos was eluted with 6 ml (23 ml) of acetonitrile/methanol (50/50, v/v)
mixture and evaporated to dryness. The extract was diluted in 2 ml of
acetonitrile/methanol (50/50, v/v). Analysis was performed with a Hewlett—
Packard (HP) model 5890 Series II gas chromatograph with EC Ni®’ detector.
Determination conditions were — t °C of column 190 °C with an increase of 30°C
min’! up to 275°C, t°C of injector 230°C and t°C of detector 300°C. The linearity
of the method was evaluated by chromatographing chlorpyrifos solution in
concentrations 0.001-1.0 pg mI'. Correlation coefficient (R°) was 0.995 and limit
of detection (LOD) and limit of quantification (LOQ) were 0.004 pg ml! and
0.01 wg mI', respectively. The average recovery for river water was 87.5% and
for groundwater 97.9%.",
journal = "Poljoprivreda i šumarstvo",
title = "Determination of chlorpyrifos in water used for agricultural production",
pages = "25-17",
number = "4",
volume = "57",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3718"
}

Lazić, S., Šunjka, D., Grahovac, N., Vuković, S.,& Jakšić, S.. (2011). Determination of chlorpyrifos in water used for agricultural production. in Poljoprivreda i šumarstvo, 57(4), 17-25.
https://hdl.handle.net/21.15107/rcub_fiver_3718

Lazić S, Šunjka D, Grahovac N, Vuković S, Jakšić S. Determination of chlorpyrifos in water used for agricultural production. in Poljoprivreda i šumarstvo. 2011;57(4):17-25.
https://hdl.handle.net/21.15107/rcub_fiver_3718 .

Lazić, S., Šunjka, D., Grahovac, Nada, Vuković, S., Jakšić, Snežana, "Determination of chlorpyrifos in water used for agricultural production" in Poljoprivreda i šumarstvo, 57, no. 4 (2011):17-25,
https://hdl.handle.net/21.15107/rcub_fiver_3718 .