TY  - JOUR
AU  - Erceg, Tamara
AU  - Aćimović, Milica
AU  - Šovljanski, Olja
AU  - Lončar, Biljana
AU  - Tomić, Ana
AU  - Pavlović, Marko
AU  - Vukić, Vladimir
AU  - Hadnađev, Miroslav
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4809
AB  - Dependence of the food industry on conventional plastic and the generation of enormous amounts of food waste caused by microbiological spoilage have been imposed as inspiration for this work, to develop active sustainable packaging for sliced cheese using the bi-layer design. Pullulan was modified using a green approach to obtain a polyanionic character in the coating formulation. Chitosan, which has a cationic character in an acidic environment, has been modified using a butyric acid to obtain an amphiphilic character. The formed active bi-layer has demonstrated an improved barrier (decreased permeability for moisture vapor 72.2 and 77.7 times) and mechanical properties (increased tensile strength value up to 3.9 and 9.4 times) compared to the monolayer films. A novel approach to microbiological control of sliced cheese has been established, which implies a synergistic effect of Helichrysum italicum essential oil (EO) and corresponding hydrolate (HY) incorporated in separated layers. This design has ensured avoiding surfactants and preserving cheese's sensory properties, prolonging its shelf-life by 50 % at least. Improvements in cheese storage conditions using this packaging lie in the improved barrier, mechanical and antimicrobial properties, the order of lamination, and a good covering of the cheese surface by spraying.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Preparation and characterization of carboxymethylated pullulan/butyric acid-modified chitosan active sustainable bi-layer coatings intended for packaging of cheese slices
IS  - 2
SP  - 134053
VL  - 277
DO  - 10.1016/j.ijbiomac.2024.134053
ER  - 

@article{
author = "Erceg, Tamara and Aćimović, Milica and Šovljanski, Olja and Lončar, Biljana and Tomić, Ana and Pavlović, Marko and Vukić, Vladimir and Hadnađev, Miroslav",
year = "2024",
abstract = "Dependence of the food industry on conventional plastic and the generation of enormous amounts of food waste caused by microbiological spoilage have been imposed as inspiration for this work, to develop active sustainable packaging for sliced cheese using the bi-layer design. Pullulan was modified using a green approach to obtain a polyanionic character in the coating formulation. Chitosan, which has a cationic character in an acidic environment, has been modified using a butyric acid to obtain an amphiphilic character. The formed active bi-layer has demonstrated an improved barrier (decreased permeability for moisture vapor 72.2 and 77.7 times) and mechanical properties (increased tensile strength value up to 3.9 and 9.4 times) compared to the monolayer films. A novel approach to microbiological control of sliced cheese has been established, which implies a synergistic effect of Helichrysum italicum essential oil (EO) and corresponding hydrolate (HY) incorporated in separated layers. This design has ensured avoiding surfactants and preserving cheese's sensory properties, prolonging its shelf-life by 50 % at least. Improvements in cheese storage conditions using this packaging lie in the improved barrier, mechanical and antimicrobial properties, the order of lamination, and a good covering of the cheese surface by spraying.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Preparation and characterization of carboxymethylated pullulan/butyric acid-modified chitosan active sustainable bi-layer coatings intended for packaging of cheese slices",
number = "2",
pages = "134053",
volume = "277",
doi = "10.1016/j.ijbiomac.2024.134053"
}

Erceg, T., Aćimović, M., Šovljanski, O., Lončar, B., Tomić, A., Pavlović, M., Vukić, V.,& Hadnađev, M.. (2024). Preparation and characterization of carboxymethylated pullulan/butyric acid-modified chitosan active sustainable bi-layer coatings intended for packaging of cheese slices. in International Journal of Biological Macromolecules
Elsevier., 277(2), 134053.
https://doi.org/10.1016/j.ijbiomac.2024.134053

Erceg T, Aćimović M, Šovljanski O, Lončar B, Tomić A, Pavlović M, Vukić V, Hadnađev M. Preparation and characterization of carboxymethylated pullulan/butyric acid-modified chitosan active sustainable bi-layer coatings intended for packaging of cheese slices. in International Journal of Biological Macromolecules. 2024;277(2):134053.
doi:10.1016/j.ijbiomac.2024.134053 .

Erceg, Tamara, Aćimović, Milica, Šovljanski, Olja, Lončar, Biljana, Tomić, Ana, Pavlović, Marko, Vukić, Vladimir, Hadnađev, Miroslav, "Preparation and characterization of carboxymethylated pullulan/butyric acid-modified chitosan active sustainable bi-layer coatings intended for packaging of cheese slices" in International Journal of Biological Macromolecules, 277, no. 2 (2024):134053,
https://doi.org/10.1016/j.ijbiomac.2024.134053 . .

TY  - JOUR
AU  - Zeremski, Tijana
AU  - Šovljanski, Olja
AU  - Vukić, Vladimir
AU  - Lončar, Biljana
AU  - Rat, Milica
AU  - Perković Vukčević, Nataša
AU  - Aćimović, Milica
AU  - Pezo, Lato
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4701
AB  - Helichrysum italicum (immortelle) essential oil is one of the most popular essential oils worldwide and it has many beneficial properties, including antimicrobial. However, in this plant, the chemical diversity of the essential oil is very pronounced. The aim of this work was to process the GC-MS results of four samples of H. italicum essential oil of Serbian origin by chemometric tools, and evaluate the antimicrobial activity in vitro and in silico. Overall, 47 compounds were identified, the most abundant were γ-curcumene, α-pinene, and ar-curcumene, followed by α-ylangene, neryl acetate, trans-caryophyllene, italicene, α-selinene, limonene, and italidiones. Although the four samples of H. italicum essential oil used in this study were obtained from different producers in Serbia, they belong to the type of essential oil rich in sesquiterpenes (γ-curcumene and ar-curcumene chemotype). In vitro antimicrobial potential showed that five were sensitive among ten strains of tested microorganisms: Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Saccharomyces cerevisiae, and Candida albicans. Therefore, these microorganism models were used further for in silico molecular docking through the mechanism of ATP-ase inhibitory activity. Results showed that among all compounds from H. italicum essential oil, neryl acetate has the highest predicted binding energy. Artificial neural network modeling (ANN) showed that two major compounds γ-curcumene and α-pinene, as well as minor compounds such as trans-β-ocimene, terpinolene, terpinene-4-ol, isoitalicene, italicene, cis-α-bergamotene, trans-α-bergamotene, italidiones, trans-β-farnesene, γ-selinene, β-selinene, α-selinene, and guaiol are responsible for the antimicrobial activity of H. italicum essential oil. The results of this study indicate that H. italicum essential oil samples rich in γ-curcumene, α-pinene, and ar-curcumene cultivated in Serbia (Balkan) have antimicrobial potential both in vitro and in silico. In addition, according to ANN modeling, the proportion of neryl acetate and other compounds detected in these samples has the potential to exhibit antimicrobial activity.
PB  - Basel : MDPI
T2  - Antibiotics
T1  - Combination of Chromatographic Analysis and Chemometric Methods with Bioactivity Evaluation of the Antibacterial Properties of Helichrysum italicum Essential Oil
IS  - 6
SP  - 499
VL  - 13
DO  - 10.3390/antibiotics13060499
ER  - 

@article{
author = "Zeremski, Tijana and Šovljanski, Olja and Vukić, Vladimir and Lončar, Biljana and Rat, Milica and Perković Vukčević, Nataša and Aćimović, Milica and Pezo, Lato",
year = "2024",
abstract = "Helichrysum italicum (immortelle) essential oil is one of the most popular essential oils worldwide and it has many beneficial properties, including antimicrobial. However, in this plant, the chemical diversity of the essential oil is very pronounced. The aim of this work was to process the GC-MS results of four samples of H. italicum essential oil of Serbian origin by chemometric tools, and evaluate the antimicrobial activity in vitro and in silico. Overall, 47 compounds were identified, the most abundant were γ-curcumene, α-pinene, and ar-curcumene, followed by α-ylangene, neryl acetate, trans-caryophyllene, italicene, α-selinene, limonene, and italidiones. Although the four samples of H. italicum essential oil used in this study were obtained from different producers in Serbia, they belong to the type of essential oil rich in sesquiterpenes (γ-curcumene and ar-curcumene chemotype). In vitro antimicrobial potential showed that five were sensitive among ten strains of tested microorganisms: Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Saccharomyces cerevisiae, and Candida albicans. Therefore, these microorganism models were used further for in silico molecular docking through the mechanism of ATP-ase inhibitory activity. Results showed that among all compounds from H. italicum essential oil, neryl acetate has the highest predicted binding energy. Artificial neural network modeling (ANN) showed that two major compounds γ-curcumene and α-pinene, as well as minor compounds such as trans-β-ocimene, terpinolene, terpinene-4-ol, isoitalicene, italicene, cis-α-bergamotene, trans-α-bergamotene, italidiones, trans-β-farnesene, γ-selinene, β-selinene, α-selinene, and guaiol are responsible for the antimicrobial activity of H. italicum essential oil. The results of this study indicate that H. italicum essential oil samples rich in γ-curcumene, α-pinene, and ar-curcumene cultivated in Serbia (Balkan) have antimicrobial potential both in vitro and in silico. In addition, according to ANN modeling, the proportion of neryl acetate and other compounds detected in these samples has the potential to exhibit antimicrobial activity.",
publisher = "Basel : MDPI",
journal = "Antibiotics",
title = "Combination of Chromatographic Analysis and Chemometric Methods with Bioactivity Evaluation of the Antibacterial Properties of Helichrysum italicum Essential Oil",
number = "6",
pages = "499",
volume = "13",
doi = "10.3390/antibiotics13060499"
}

Zeremski, T., Šovljanski, O., Vukić, V., Lončar, B., Rat, M., Perković Vukčević, N., Aćimović, M.,& Pezo, L.. (2024). Combination of Chromatographic Analysis and Chemometric Methods with Bioactivity Evaluation of the Antibacterial Properties of Helichrysum italicum Essential Oil. in Antibiotics
Basel : MDPI., 13(6), 499.
https://doi.org/10.3390/antibiotics13060499

Zeremski T, Šovljanski O, Vukić V, Lončar B, Rat M, Perković Vukčević N, Aćimović M, Pezo L. Combination of Chromatographic Analysis and Chemometric Methods with Bioactivity Evaluation of the Antibacterial Properties of Helichrysum italicum Essential Oil. in Antibiotics. 2024;13(6):499.
doi:10.3390/antibiotics13060499 .

Zeremski, Tijana, Šovljanski, Olja, Vukić, Vladimir, Lončar, Biljana, Rat, Milica, Perković Vukčević, Nataša, Aćimović, Milica, Pezo, Lato, "Combination of Chromatographic Analysis and Chemometric Methods with Bioactivity Evaluation of the Antibacterial Properties of Helichrysum italicum Essential Oil" in Antibiotics, 13, no. 6 (2024):499,
https://doi.org/10.3390/antibiotics13060499 . .

TY  - JOUR
AU  - Lazarević, Jovan
AU  - Aćimović, Milica
AU  - Đurović-Pejčev, Rada
AU  - Lončar, Biljana
AU  - Vukić, Vladimir
AU  - Pezo, Lato
AU  - Roljević Nikolić, Svetlana
AU  - Vrbničanin, Sava
AU  - Božić, Dragana
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4645
AB  - The present study aimed to evaluate garden angelica (Angelica archangelica L.) root essential oil composition modulated by weed management techniques. Eight different treatments, including weed free (manual weeding removal), weedy check (untreated control), two organic mulches: sawdust and straw, two physical methods mulches: agro-textile foil and silver-brown foil, and two herbicides: Metamitron and Aclonifen, were employed in open field growing A. archangelica. The composition of the root essential oil was analysed by gas chromatography-mass spectrometry. The results revealed that main compounds in A. archangelica root essential oil was β-phellandrene (6.7–17.2%) and α-pinene (7.8–17.2%), followed by δ-3-carene (4.2–14.1%) and p-cymene (6.4–10.5%). According to molecular docking results and binding energy calculations, there are several compounds present in A. archangelica essential oil that could have modulatory effects on inflammatory pathways. However, α-pinene, osthol, α-terpinol, p-cymene, and β-phellandrene seem to be the most effective anti-inflammatory compounds from the examined oil. Application of herbicides in weed management significantly influences the accumulation α-pinene as a dominant compound in A. archangelica essential oil, while β-phellandrene dominant in straw weed management. Moreover, α-terpineol was the most abundant in sawdust weed management treatment, while osthol was dominant in weed free treatment. In conclusion, organic mulches, especially sawdust, can be recommended for the production of A. archangelica roots with the highest essential oil content, while straw as an organic mulch gives the highest concentration of main compounds with promising anti-inflammatory potential.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Linking weed control techniques to anti-inflammatory potential: Comparative analysis of Angelica archangelica L. root essential oil profiles
SP  - 118656
VL  - 216
DO  - 10.1016/j.indcrop.2024.118656
ER  - 

@article{
author = "Lazarević, Jovan and Aćimović, Milica and Đurović-Pejčev, Rada and Lončar, Biljana and Vukić, Vladimir and Pezo, Lato and Roljević Nikolić, Svetlana and Vrbničanin, Sava and Božić, Dragana",
year = "2024",
abstract = "The present study aimed to evaluate garden angelica (Angelica archangelica L.) root essential oil composition modulated by weed management techniques. Eight different treatments, including weed free (manual weeding removal), weedy check (untreated control), two organic mulches: sawdust and straw, two physical methods mulches: agro-textile foil and silver-brown foil, and two herbicides: Metamitron and Aclonifen, were employed in open field growing A. archangelica. The composition of the root essential oil was analysed by gas chromatography-mass spectrometry. The results revealed that main compounds in A. archangelica root essential oil was β-phellandrene (6.7–17.2%) and α-pinene (7.8–17.2%), followed by δ-3-carene (4.2–14.1%) and p-cymene (6.4–10.5%). According to molecular docking results and binding energy calculations, there are several compounds present in A. archangelica essential oil that could have modulatory effects on inflammatory pathways. However, α-pinene, osthol, α-terpinol, p-cymene, and β-phellandrene seem to be the most effective anti-inflammatory compounds from the examined oil. Application of herbicides in weed management significantly influences the accumulation α-pinene as a dominant compound in A. archangelica essential oil, while β-phellandrene dominant in straw weed management. Moreover, α-terpineol was the most abundant in sawdust weed management treatment, while osthol was dominant in weed free treatment. In conclusion, organic mulches, especially sawdust, can be recommended for the production of A. archangelica roots with the highest essential oil content, while straw as an organic mulch gives the highest concentration of main compounds with promising anti-inflammatory potential.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Linking weed control techniques to anti-inflammatory potential: Comparative analysis of Angelica archangelica L. root essential oil profiles",
pages = "118656",
volume = "216",
doi = "10.1016/j.indcrop.2024.118656"
}

Lazarević, J., Aćimović, M., Đurović-Pejčev, R., Lončar, B., Vukić, V., Pezo, L., Roljević Nikolić, S., Vrbničanin, S.,& Božić, D.. (2024). Linking weed control techniques to anti-inflammatory potential: Comparative analysis of Angelica archangelica L. root essential oil profiles. in Industrial Crops and Products
Elsevier., 216, 118656.
https://doi.org/10.1016/j.indcrop.2024.118656

Lazarević J, Aćimović M, Đurović-Pejčev R, Lončar B, Vukić V, Pezo L, Roljević Nikolić S, Vrbničanin S, Božić D. Linking weed control techniques to anti-inflammatory potential: Comparative analysis of Angelica archangelica L. root essential oil profiles. in Industrial Crops and Products. 2024;216:118656.
doi:10.1016/j.indcrop.2024.118656 .

Lazarević, Jovan, Aćimović, Milica, Đurović-Pejčev, Rada, Lončar, Biljana, Vukić, Vladimir, Pezo, Lato, Roljević Nikolić, Svetlana, Vrbničanin, Sava, Božić, Dragana, "Linking weed control techniques to anti-inflammatory potential: Comparative analysis of Angelica archangelica L. root essential oil profiles" in Industrial Crops and Products, 216 (2024):118656,
https://doi.org/10.1016/j.indcrop.2024.118656 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Semerdijeva, Ivanka
AU  - Zheljazkov, Valtcho
AU  - Rat, Milica
AU  - Stanković Jeremić, Jovana
AU  - Lončar, Biljana
AU  - Vukić, Vladimir
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Pezo, Lato
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4054
AB  - This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile.
PB  - Elsevier
T2  - Biochemical Systematics and Ecology
T1  - Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm
SP  - 104679
VL  - 110
DO  - 10.1016/j.bse.2023.104679
ER  - 

@article{
author = "Aćimović, Milica and Semerdijeva, Ivanka and Zheljazkov, Valtcho and Rat, Milica and Stanković Jeremić, Jovana and Lončar, Biljana and Vukić, Vladimir and Radovanović, Katarina and Gavarić, Neda and Pezo, Lato",
year = "2023",
abstract = "This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile.",
publisher = "Elsevier",
journal = "Biochemical Systematics and Ecology",
title = "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm",
pages = "104679",
volume = "110",
doi = "10.1016/j.bse.2023.104679"
}

Aćimović, M., Semerdijeva, I., Zheljazkov, V., Rat, M., Stanković Jeremić, J., Lončar, B., Vukić, V., Radovanović, K., Gavarić, N.,& Pezo, L.. (2023). Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology
Elsevier., 110, 104679.
https://doi.org/10.1016/j.bse.2023.104679

Aćimović M, Semerdijeva I, Zheljazkov V, Rat M, Stanković Jeremić J, Lončar B, Vukić V, Radovanović K, Gavarić N, Pezo L. Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology. 2023;110:104679.
doi:10.1016/j.bse.2023.104679 .

Aćimović, Milica, Semerdijeva, Ivanka, Zheljazkov, Valtcho, Rat, Milica, Stanković Jeremić, Jovana, Lončar, Biljana, Vukić, Vladimir, Radovanović, Katarina, Gavarić, Neda, Pezo, Lato, "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm" in Biochemical Systematics and Ecology, 110 (2023):104679,
https://doi.org/10.1016/j.bse.2023.104679 . .