TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3738
AB  - Waitea circinata var. zeae (anamorf Rhizoctonia zeae) je značajan patogen uglavnom onokotiledonih biljaka, koji je prisutan u mnogim delovima sveta, a održava se u zemljištu duži niz godina. Poslednjih godina krug domaćina W. circinata var. zeae širi se i na neke dikotiledone biljke. U okviru nedavnih istraživanja u Srbiji W. circinata var. zeae je utvrđen kao prouzrokovač truleži korena i korenovog vrata, i propadanja biljaka kupusa i uljane repice na lokalitetima Futog i Rimski Šančevi. U radu su sistematizovani publikovani podaci o rasprostranjenosti, krugu domaćina, načinu održavanja i širenja, kao i suzbijanju W. circinata var. zeae, kao i rezultati istraživanja koja su obavljena u Srbiji.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
T2  - Biljni lekar / Plant Doctor
T1  - Waitea circinata var. zeae – prouzrokovač truleži korena kupusa i uljane repice
EP  - 593
IS  - 4
SP  - 585
VL  - 51
DO  - 10.5937/BiljLek2304585V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2023",
abstract = "Waitea circinata var. zeae (anamorf Rhizoctonia zeae) je značajan patogen uglavnom onokotiledonih biljaka, koji je prisutan u mnogim delovima sveta, a održava se u zemljištu duži niz godina. Poslednjih godina krug domaćina W. circinata var. zeae širi se i na neke dikotiledone biljke. U okviru nedavnih istraživanja u Srbiji W. circinata var. zeae je utvrđen kao prouzrokovač truleži korena i korenovog vrata, i propadanja biljaka kupusa i uljane repice na lokalitetima Futog i Rimski Šančevi. U radu su sistematizovani publikovani podaci o rasprostranjenosti, krugu domaćina, načinu održavanja i širenja, kao i suzbijanju W. circinata var. zeae, kao i rezultati istraživanja koja su obavljena u Srbiji.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Biljni lekar / Plant Doctor",
title = "Waitea circinata var. zeae – prouzrokovač truleži korena kupusa i uljane repice",
pages = "593-585",
number = "4",
volume = "51",
doi = "10.5937/BiljLek2304585V"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2023). Waitea circinata var. zeae – prouzrokovač truleži korena kupusa i uljane repice. in Biljni lekar / Plant Doctor
Novi Sad : University of Novi Sad, Faculty of Agriculture., 51(4), 585-593.
https://doi.org/10.5937/BiljLek2304585V

Vojvodić M, Tanović B, Mitrović P, Vico I, Bulajić A. Waitea circinata var. zeae – prouzrokovač truleži korena kupusa i uljane repice. in Biljni lekar / Plant Doctor. 2023;51(4):585-593.
doi:10.5937/BiljLek2304585V .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Waitea circinata var. zeae – prouzrokovač truleži korena kupusa i uljane repice" in Biljni lekar / Plant Doctor, 51, no. 4 (2023):585-593,
https://doi.org/10.5937/BiljLek2304585V . .

TY  - JOUR
AU  - Dragić, Vasiljka
AU  - Miljaković, Dragana
AU  - Marinković, Jelena
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Vasin, Jovica
AU  - Bulajić, Aleksandra
AU  - Vojvodić, Mira
AU  - Ivanović, Milan
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3251
AB  - Grey mould, which is caused by Botrytis cinerea Pers., is among the most damaging diseases of cultivated plants worldwide. The use of fungicides dominates in the protection of tomatoes against grey mould. Due to the emergence of resistance of pathogens to the active ingredients of fungicides, the possibility of using bacteria of the genus Bacillus was studied. A total of 666 isolates were isolated from different agricultural soils, and a preliminary screening dual culture
test with Alternaria dauci was done. A total of 77 isolates were dual culture tested against Fusarium tricinctum and F. proliferatum. Based on preliminary dual culture testing, eight Bacillus isolates were selected for testing their biological potential against two isolates of B. cinerea. The effect of Bacillus spp. isolates was studied for seed germination and plant growth promotion (PGP) on the seedlings of tomato (Solanum lycopersicum L.). Using molecular identification (16S rDNA), five bacterial isolates were identified as Bacillus subtilis, two isolates as B. amyloliquefaciens, and one
isolate as B. pumilus. Using the Internal transcribed spacer (ITS) region, two fungal isolates from an infected leaf and a tomato fruit were identified as B. cinerea. The results of the dual culture test showed that eight bacterial isolates could significantly inhibit the growth of B. cinerea mycelia from 50% to 80%. Two isolates B. amyloliquefaciens 28.3 and B. amyloliquefaciens 21/IV increased seed germination by 85.66% and 86.16%, respectively, compared to the control (82.66%). All bacterial isolates positively affected the morphological parameters of the seedlings. In the greenhouse experiment, three isolates B. subtillis 20.10, B. amyloliquefaciens 28.3, and B. amyloliquefaciens 21/IV and their mixture increased all measured PGP parameters. Isolates Bac 20.10 and Bac 28.3 reduced the severity of grey mould disease by 27.47% and 30.36%, respectively. These results show that Bacillus isolates have the potential for biological control of grey mould; also, that they have a positive effect on the growth of tomato plants.
PB  - Lithuanian Research Centre for Agriculture and Forestry, Vytautas Magnus University
T2  - Zemdirbyste-Agriculture
T1  - Biocontrol of Botrytis cinerea and promotion of tomato growth by local soil-borne Bacillus isolates
EP  - 164
IS  - 2
SP  - 157
VL  - 109
DO  - 10.13080/z-a.2022.109.020
ER  - 

@article{
author = "Dragić, Vasiljka and Miljaković, Dragana and Marinković, Jelena and Ignjatov, Maja and Milošević, Dragana and Vasin, Jovica and Bulajić, Aleksandra and Vojvodić, Mira and Ivanović, Milan",
year = "2022",
abstract = "Grey mould, which is caused by Botrytis cinerea Pers., is among the most damaging diseases of cultivated plants worldwide. The use of fungicides dominates in the protection of tomatoes against grey mould. Due to the emergence of resistance of pathogens to the active ingredients of fungicides, the possibility of using bacteria of the genus Bacillus was studied. A total of 666 isolates were isolated from different agricultural soils, and a preliminary screening dual culture
test with Alternaria dauci was done. A total of 77 isolates were dual culture tested against Fusarium tricinctum and F. proliferatum. Based on preliminary dual culture testing, eight Bacillus isolates were selected for testing their biological potential against two isolates of B. cinerea. The effect of Bacillus spp. isolates was studied for seed germination and plant growth promotion (PGP) on the seedlings of tomato (Solanum lycopersicum L.). Using molecular identification (16S rDNA), five bacterial isolates were identified as Bacillus subtilis, two isolates as B. amyloliquefaciens, and one
isolate as B. pumilus. Using the Internal transcribed spacer (ITS) region, two fungal isolates from an infected leaf and a tomato fruit were identified as B. cinerea. The results of the dual culture test showed that eight bacterial isolates could significantly inhibit the growth of B. cinerea mycelia from 50% to 80%. Two isolates B. amyloliquefaciens 28.3 and B. amyloliquefaciens 21/IV increased seed germination by 85.66% and 86.16%, respectively, compared to the control (82.66%). All bacterial isolates positively affected the morphological parameters of the seedlings. In the greenhouse experiment, three isolates B. subtillis 20.10, B. amyloliquefaciens 28.3, and B. amyloliquefaciens 21/IV and their mixture increased all measured PGP parameters. Isolates Bac 20.10 and Bac 28.3 reduced the severity of grey mould disease by 27.47% and 30.36%, respectively. These results show that Bacillus isolates have the potential for biological control of grey mould; also, that they have a positive effect on the growth of tomato plants.",
publisher = "Lithuanian Research Centre for Agriculture and Forestry, Vytautas Magnus University",
journal = "Zemdirbyste-Agriculture",
title = "Biocontrol of Botrytis cinerea and promotion of tomato growth by local soil-borne Bacillus isolates",
pages = "164-157",
number = "2",
volume = "109",
doi = "10.13080/z-a.2022.109.020"
}

Dragić, V., Miljaković, D., Marinković, J., Ignjatov, M., Milošević, D., Vasin, J., Bulajić, A., Vojvodić, M.,& Ivanović, M.. (2022). Biocontrol of Botrytis cinerea and promotion of tomato growth by local soil-borne Bacillus isolates. in Zemdirbyste-Agriculture
Lithuanian Research Centre for Agriculture and Forestry, Vytautas Magnus University., 109(2), 157-164.
https://doi.org/10.13080/z-a.2022.109.020

Dragić V, Miljaković D, Marinković J, Ignjatov M, Milošević D, Vasin J, Bulajić A, Vojvodić M, Ivanović M. Biocontrol of Botrytis cinerea and promotion of tomato growth by local soil-borne Bacillus isolates. in Zemdirbyste-Agriculture. 2022;109(2):157-164.
doi:10.13080/z-a.2022.109.020 .

Dragić, Vasiljka, Miljaković, Dragana, Marinković, Jelena, Ignjatov, Maja, Milošević, Dragana, Vasin, Jovica, Bulajić, Aleksandra, Vojvodić, Mira, Ivanović, Milan, "Biocontrol of Botrytis cinerea and promotion of tomato growth by local soil-borne Bacillus isolates" in Zemdirbyste-Agriculture, 109, no. 2 (2022):157-164,
https://doi.org/10.13080/z-a.2022.109.020 . .

TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2811
AB  - Waitea circinata Warcup & P.H.B. Talbot (multinuclear Rhizoctonia spp.) is a widespread, soil-borne plant pathogen causing root rot mainly of monocotyledonous plants. Among five described varieties, the most common and widespread is W. circinata var. zeae Toda, Mushika, T. Hayak., Tanaka, Tani & Hyakum. Besides monocotyledonous hosts, a natural infection of a limited number of dicotyledonous plants, including soybeans, beans, peas, sugar beets and carrots were recorded recently. During 2017, cabbage and oilseed rape plants showing symptoms of root and root neck rot, sampled at the localities of Futog and Rimski Šančevi (Serbia), were proved to be infected with W. circinata var. zeae. This was the first record of natural infection of plants from Brassicaceae family worldwide. Two selected isolates from cabbage (299-17) and oilseed rape (300-17) formed fast-growing orange colonies with red sclerotia and multinucleate hyphae with a characteristic branching pattern. The identification was further confirmed by BLAST analysis of sequences of ITS rDNA, RPB2, LSU and β-tubulin genomic regions. Koch’s postulates were fulfilled after inoculation of cabbage and oilseed rape seedlings, while an experimental host range was determined by inoculations of 14 different plant species from 8 families.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Pathogenic potential of Waitea circinata var. zeae
EP  - 84
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2811
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2022",
abstract = "Waitea circinata Warcup & P.H.B. Talbot (multinuclear Rhizoctonia spp.) is a widespread, soil-borne plant pathogen causing root rot mainly of monocotyledonous plants. Among five described varieties, the most common and widespread is W. circinata var. zeae Toda, Mushika, T. Hayak., Tanaka, Tani & Hyakum. Besides monocotyledonous hosts, a natural infection of a limited number of dicotyledonous plants, including soybeans, beans, peas, sugar beets and carrots were recorded recently. During 2017, cabbage and oilseed rape plants showing symptoms of root and root neck rot, sampled at the localities of Futog and Rimski Šančevi (Serbia), were proved to be infected with W. circinata var. zeae. This was the first record of natural infection of plants from Brassicaceae family worldwide. Two selected isolates from cabbage (299-17) and oilseed rape (300-17) formed fast-growing orange colonies with red sclerotia and multinucleate hyphae with a characteristic branching pattern. The identification was further confirmed by BLAST analysis of sequences of ITS rDNA, RPB2, LSU and β-tubulin genomic regions. Koch’s postulates were fulfilled after inoculation of cabbage and oilseed rape seedlings, while an experimental host range was determined by inoculations of 14 different plant species from 8 families.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Pathogenic potential of Waitea circinata var. zeae",
pages = "84-83",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2811"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2022). Pathogenic potential of Waitea circinata var. zeae. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 83-84.
https://hdl.handle.net/21.15107/rcub_fiver_2811

Vojvodić M, Tanović B, Mitrović P, Vico I, Bulajić A. Pathogenic potential of Waitea circinata var. zeae. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:83-84.
https://hdl.handle.net/21.15107/rcub_fiver_2811 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Pathogenic potential of Waitea circinata var. zeae" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):83-84,
https://hdl.handle.net/21.15107/rcub_fiver_2811 .

TY  - CONF
AU  - Dragić, Vasiljka
AU  - Miljaković, Dragana
AU  - Marinković, Jelena
AU  - Vlajić, Slobodan
AU  - Bulajić, Aleksandra
AU  - Vojvodić, Mira
AU  - Menković, Jelena
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2526
AB  - Paradajz je jedna od najvažnijih povrtarskih biljaka čiju proizvodnju može ugroziti veći broj prouzrokovača bolesti, smanjujući prinos i kvalitet ploda. Jedan od najznačajnijih patogena paradajza je gljiva Botrytis cinerea - prouzrokovač sive truleži paradajza. Uprkos raznim preventivnim metodama, primena fungicida i dalje predstavlja najvažniju meru kontrole ovog patogena. Međutim, kontinuirana primena hemikalija izaziva zabrinutost potrošača zbog ostataka preparata na plodovima paradajza i njegovog štetnog uticaja na zdravlje ljudi. Jedna od alternativnih mera u zaštiti biljaka od prouzrokovača sive truleži jeste primena različitih antagonističkih bakterija, između ostalih i iz roda Bacillus. Osim uloge u biokontroli, poznato je da pomenute bakterije mogu stimulisati i rast biljaka. Ciljevi istraživanja bili su izolacija autohtonih sojeva Bacillus spp. iz zemljišta, ispitivanje njihove antifungalne aktivnosti prema dva izolata B. cinerea i proučavanje njihovog uticaja na klijavost semena paradajza i morfološke parametre klijanaca. Antifungalni efekat Bacillus spp. testitan je u in vitro uslovima, primenom metode dvojne kultivacije, u tri ponavljanja. Nakon 7 dana inkubacije pri 28ºC izračunat je procenat inhibicije rasta micelije (Percentage of Growth Inhibition, PGI) i zona inhibicije. Ispitivanje uticaja antagonističkih bakterija na klijavost semena paradajza izvršeno je standardnim testom klijavosti. Seme paradajza, sorte Novosadski jabučar, inokulisano je potapanjem u suspenziju bakterija (108 ćel/ml), dok je seme u kontroli potapano u 0,2% rastvor kalijum nitrata (KNO3). Efekat je ocenjen naklijavanjem 100 semena u Petri posudi (R=140 mm) u četiri ponavljanja. Očitavanje klijavosti semena izvršeno je nakon 14 dana. Iz svakog ponavljanja, slučajnim izborom odabrano je po 10 klijanaca i merena je dužina nadzemnog dela i korena (cm), kao i sveža i suva masa nadzemnog dela i korena (g). Primenom morfoloških i molekularnih metoda, pet bakterijskih sojeva identifikovano je kao Bacillus subtilis, dva soja kao B. amyloliquefaciens i jedan kao B. pumilus. U testu dvojne kultivacije svih osam sojeva Bacillus spp. ispoljili su snažan antifungalni efekat prema oba izolata B. cinerea, sa PGI vrednostima od 50% do 80% i zonom inhibicije od 1,33 mm do 17,33 mm. Najveći procenat klijavosti semena dobijen je inokulacijom sojevima B. amyloliquefaciens (85,66% i 86,16%) u odnosu na kontrolu (82,66%). Jedan soj B. subtillis povećao je dužinu nadzemnog dela za 25,50%, dok je drugi soj iste vrste povećao dužinu korena za 17,62%. Dalje, sojevi B. amyloliquefaciens, B. pumilus, kao i tri soja B. subtillis statistički značajno su povećali svežu masu nadzemnog dela (12,90 - 22,22%). Sojevi B. pumilus i B. subtillis povećali su i svežu masu korena (2,56 - 5,12%). Inicijalni rezultati ukazuju da autohtoni izolati Bacillus spp. iz zemljišta imaju snažan potencijal za biokontrolu i podsticanje rasta biljaka paradajza.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, 22-25. Novembar 2021, Zlatibor
T1  - Antagonistički potencijal Bacillus spp. prema prouzrokovaču sive truleži paradajza (Botrytis cinerea) i uticaj na klijavost i morfološke parametre klijanaca
EP  - 31
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2526
ER  - 

@conference{
author = "Dragić, Vasiljka and Miljaković, Dragana and Marinković, Jelena and Vlajić, Slobodan and Bulajić, Aleksandra and Vojvodić, Mira and Menković, Jelena and Prokić, Anđelka and Obradović, Aleksa and Ivanović, Milan",
year = "2021",
abstract = "Paradajz je jedna od najvažnijih povrtarskih biljaka čiju proizvodnju može ugroziti veći broj prouzrokovača bolesti, smanjujući prinos i kvalitet ploda. Jedan od najznačajnijih patogena paradajza je gljiva Botrytis cinerea - prouzrokovač sive truleži paradajza. Uprkos raznim preventivnim metodama, primena fungicida i dalje predstavlja najvažniju meru kontrole ovog patogena. Međutim, kontinuirana primena hemikalija izaziva zabrinutost potrošača zbog ostataka preparata na plodovima paradajza i njegovog štetnog uticaja na zdravlje ljudi. Jedna od alternativnih mera u zaštiti biljaka od prouzrokovača sive truleži jeste primena različitih antagonističkih bakterija, između ostalih i iz roda Bacillus. Osim uloge u biokontroli, poznato je da pomenute bakterije mogu stimulisati i rast biljaka. Ciljevi istraživanja bili su izolacija autohtonih sojeva Bacillus spp. iz zemljišta, ispitivanje njihove antifungalne aktivnosti prema dva izolata B. cinerea i proučavanje njihovog uticaja na klijavost semena paradajza i morfološke parametre klijanaca. Antifungalni efekat Bacillus spp. testitan je u in vitro uslovima, primenom metode dvojne kultivacije, u tri ponavljanja. Nakon 7 dana inkubacije pri 28ºC izračunat je procenat inhibicije rasta micelije (Percentage of Growth Inhibition, PGI) i zona inhibicije. Ispitivanje uticaja antagonističkih bakterija na klijavost semena paradajza izvršeno je standardnim testom klijavosti. Seme paradajza, sorte Novosadski jabučar, inokulisano je potapanjem u suspenziju bakterija (108 ćel/ml), dok je seme u kontroli potapano u 0,2% rastvor kalijum nitrata (KNO3). Efekat je ocenjen naklijavanjem 100 semena u Petri posudi (R=140 mm) u četiri ponavljanja. Očitavanje klijavosti semena izvršeno je nakon 14 dana. Iz svakog ponavljanja, slučajnim izborom odabrano je po 10 klijanaca i merena je dužina nadzemnog dela i korena (cm), kao i sveža i suva masa nadzemnog dela i korena (g). Primenom morfoloških i molekularnih metoda, pet bakterijskih sojeva identifikovano je kao Bacillus subtilis, dva soja kao B. amyloliquefaciens i jedan kao B. pumilus. U testu dvojne kultivacije svih osam sojeva Bacillus spp. ispoljili su snažan antifungalni efekat prema oba izolata B. cinerea, sa PGI vrednostima od 50% do 80% i zonom inhibicije od 1,33 mm do 17,33 mm. Najveći procenat klijavosti semena dobijen je inokulacijom sojevima B. amyloliquefaciens (85,66% i 86,16%) u odnosu na kontrolu (82,66%). Jedan soj B. subtillis povećao je dužinu nadzemnog dela za 25,50%, dok je drugi soj iste vrste povećao dužinu korena za 17,62%. Dalje, sojevi B. amyloliquefaciens, B. pumilus, kao i tri soja B. subtillis statistički značajno su povećali svežu masu nadzemnog dela (12,90 - 22,22%). Sojevi B. pumilus i B. subtillis povećali su i svežu masu korena (2,56 - 5,12%). Inicijalni rezultati ukazuju da autohtoni izolati Bacillus spp. iz zemljišta imaju snažan potencijal za biokontrolu i podsticanje rasta biljaka paradajza.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, 22-25. Novembar 2021, Zlatibor",
title = "Antagonistički potencijal Bacillus spp. prema prouzrokovaču sive truleži paradajza (Botrytis cinerea) i uticaj na klijavost i morfološke parametre klijanaca",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2526"
}

Dragić, V., Miljaković, D., Marinković, J., Vlajić, S., Bulajić, A., Vojvodić, M., Menković, J., Prokić, A., Obradović, A.,& Ivanović, M.. (2021). Antagonistički potencijal Bacillus spp. prema prouzrokovaču sive truleži paradajza (Botrytis cinerea) i uticaj na klijavost i morfološke parametre klijanaca. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, 22-25. Novembar 2021, Zlatibor
Beograd : Društvo za zaštitu bilja Srbije., 31-31.
https://hdl.handle.net/21.15107/rcub_fiver_2526

Dragić V, Miljaković D, Marinković J, Vlajić S, Bulajić A, Vojvodić M, Menković J, Prokić A, Obradović A, Ivanović M. Antagonistički potencijal Bacillus spp. prema prouzrokovaču sive truleži paradajza (Botrytis cinerea) i uticaj na klijavost i morfološke parametre klijanaca. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, 22-25. Novembar 2021, Zlatibor. 2021;:31-31.
https://hdl.handle.net/21.15107/rcub_fiver_2526 .

Dragić, Vasiljka, Miljaković, Dragana, Marinković, Jelena, Vlajić, Slobodan, Bulajić, Aleksandra, Vojvodić, Mira, Menković, Jelena, Prokić, Anđelka, Obradović, Aleksa, Ivanović, Milan, "Antagonistički potencijal Bacillus spp. prema prouzrokovaču sive truleži paradajza (Botrytis cinerea) i uticaj na klijavost i morfološke parametre klijanaca" in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, 22-25. Novembar 2021, Zlatibor (2021):31-31,
https://hdl.handle.net/21.15107/rcub_fiver_2526 .

TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/4245
AB  - Kupus i uljana repica su dva vrlo značajna useva u našoj zemlji i njihova proizvodnja ugrožena je brojnim patogenim organizmima. Višejedarne anamorfne Rhizoctonia spp. sa teleomorfom opisanim kao Waitea circinata su biljni patogeni koji se dugo održavaju u zemljištu i rasprostranjeni su i vrlo štetni širom sveta. Od pet opisanih varijeteta W. circinata var. zeae je najrasprostranjeniji. Za sada poznat krug domaćina W. circinata var. zeae obuhvata monokotiledone i mali broj dikotiledonih biljaka, među kojima soju, pasulj, grašak, šećernu repu i mrkvu.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.
T1  - Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji
EP  - 20
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4245
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2021",
abstract = "Kupus i uljana repica su dva vrlo značajna useva u našoj zemlji i njihova proizvodnja ugrožena je brojnim patogenim organizmima. Višejedarne anamorfne Rhizoctonia spp. sa teleomorfom opisanim kao Waitea circinata su biljni patogeni koji se dugo održavaju u zemljištu i rasprostranjeni su i vrlo štetni širom sveta. Od pet opisanih varijeteta W. circinata var. zeae je najrasprostranjeniji. Za sada poznat krug domaćina W. circinata var. zeae obuhvata monokotiledone i mali broj dikotiledonih biljaka, među kojima soju, pasulj, grašak, šećernu repu i mrkvu.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.",
title = "Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji",
pages = "20-19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4245"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2021). Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 19-20.
https://hdl.handle.net/21.15107/rcub_fiver_4245

Vojvodić M, Tanović B, Mitrović P, Vico I, Bulajić A. Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.. 2021;:19-20.
https://hdl.handle.net/21.15107/rcub_fiver_4245 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji" in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021. (2021):19-20,
https://hdl.handle.net/21.15107/rcub_fiver_4245 .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3751
AB  - Cabbage, a widely used and popular vegetable, and oilseed rape, the second most valuable oilseed crop in the world, are two important species from the Brassicaceae family. Two geographically separated outbreaks of cabbage and oilseed rape root rot with estimated incidence of 15 and 20%, respectively, were recorded during 2017 in the Vojvodina region, Serbia. Twelve hyphal-tip isolates were obtained from symptomatic cabbage and oilseed rape plants and identified as Waitea circinata var. zeae based on morphological and molecular features. This indicates that W. circinata var. zeae has expanded its host range to the Brassicaceae family. Sequence analyses of internal transcribed spacer (ITS) and large subunit of the ribosomal DNA, RPB2, and B-tubulin genes revealed the highest similarity with multiple W. circinata var. zeae. Neighbor-joining analyses of ITS sequences resulted in a phylogenetic tree with one welldefined branch of W. circinata var. zeae, with two separate groups. All Serbian isolates and the majority of isolates originating from natural infection of dicotyledonous plants grouped together in group I. Following artificial inoculation, W. circinata var. zeae isolates caused mild to medium root necrosis of seedlings of 2 monocotyledonous and 12 dicotyledonous plant species, implying a wider host range than was known for W. circinata var. zeae. Additionally, this is the first occurrence of W. circinata var. zeae on dicotyledonous host plants in Europe. Because cabbage and oilseed rape are important crops grown worldwide, the occurrence of this new soilborne pathogen with a broad host range imposes the necessity for changes in routine disease control practices, particularly crop rotation.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape
EP  - 796
SP  - 787
VL  - 105
DO  - 10.1094/PDIS-05-20-0942-RE
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2021",
abstract = "Cabbage, a widely used and popular vegetable, and oilseed rape, the second most valuable oilseed crop in the world, are two important species from the Brassicaceae family. Two geographically separated outbreaks of cabbage and oilseed rape root rot with estimated incidence of 15 and 20%, respectively, were recorded during 2017 in the Vojvodina region, Serbia. Twelve hyphal-tip isolates were obtained from symptomatic cabbage and oilseed rape plants and identified as Waitea circinata var. zeae based on morphological and molecular features. This indicates that W. circinata var. zeae has expanded its host range to the Brassicaceae family. Sequence analyses of internal transcribed spacer (ITS) and large subunit of the ribosomal DNA, RPB2, and B-tubulin genes revealed the highest similarity with multiple W. circinata var. zeae. Neighbor-joining analyses of ITS sequences resulted in a phylogenetic tree with one welldefined branch of W. circinata var. zeae, with two separate groups. All Serbian isolates and the majority of isolates originating from natural infection of dicotyledonous plants grouped together in group I. Following artificial inoculation, W. circinata var. zeae isolates caused mild to medium root necrosis of seedlings of 2 monocotyledonous and 12 dicotyledonous plant species, implying a wider host range than was known for W. circinata var. zeae. Additionally, this is the first occurrence of W. circinata var. zeae on dicotyledonous host plants in Europe. Because cabbage and oilseed rape are important crops grown worldwide, the occurrence of this new soilborne pathogen with a broad host range imposes the necessity for changes in routine disease control practices, particularly crop rotation.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape",
pages = "796-787",
volume = "105",
doi = "10.1094/PDIS-05-20-0942-RE"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2021). Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape. in Plant Disease
American Phytopathological Society., 105, 787-796.
https://doi.org/10.1094/PDIS-05-20-0942-RE

Vojvodić M, Tanović B, Mitrović P, Vico I, Bulajić A. Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape. in Plant Disease. 2021;105:787-796.
doi:10.1094/PDIS-05-20-0942-RE .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape" in Plant Disease, 105 (2021):787-796,
https://doi.org/10.1094/PDIS-05-20-0942-RE . .

TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Lazić, Dejan
AU  - Stevanović, Miloš
AU  - Bulajić, Aleksandra
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2373
AB  - Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
T1  - Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia
EP  - 32
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2373
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Lazić, Dejan and Stevanović, Miloš and Bulajić, Aleksandra",
year = "2020",
abstract = "Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina",
title = "Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia",
pages = "32-31",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2373"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I., Lazić, D., Stevanović, M.,& Bulajić, A.. (2020). Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia. in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_fiver_2373

Vojvodić M, Tanović B, Mitrović P, Vico I, Lazić D, Stevanović M, Bulajić A. Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia. in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina. 2020;:31-32.
https://hdl.handle.net/21.15107/rcub_fiver_2373 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Lazić, Dejan, Stevanović, Miloš, Bulajić, Aleksandra, "Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia" in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina (2020):31-32,
https://hdl.handle.net/21.15107/rcub_fiver_2373 .

TY  - JOUR
AU  - Trkulja, Vojislav
AU  - Perviz, Mehira
AU  - Mitrović, Petar
AU  - Vojvodić, Mira
AU  - Bulajić, Aleksandra
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2025
AB  - Species of the genus Alternaria are among the most important plant pathogens that occur worldwide on many host plants, as well as plants of the Apiaceae family, including important vegetables such as carrots, parsley, celery, parsnips and fennel, and popular herbs such as anise, cumin, dill, coriander and others. These plants are attacked by different species of phytopathogenic fungi of the genus Alternaria, and this paper offers current review of nine different species. However, there is still insufficient information on both these species and the nu-merous interactions between them and their host plants and environmental factors. This information are necessary as the basis for adequate disease control measures and thus enabling successful and profitable cultivation of these high valued crops. Therefore, we present pathogen profiles of A. dauci, A. radicina, A. carotiincultae, A. petroselini, A. selini, A. smyrnii, A. alternata, A. longipes and A. burnsii as pathogens of Apiaceae plants, as well as their full current names and synonyms, taxonomic position, distribution and symptoms these pathogens cause to their host plants, as well as their biology and mode of transmission and spread, with special emphasis on control measures.
AB  - Vrste roda Alternaria spadaju među najvažnije biljne patogene koji se pojavljuju širom svijeta na mnogim biljkama domaćinima, uključujući i biljke iz familije Apiaceae. Među njima posebno su značajne povrtrarske biljke kao što su mrkva, peršun, celer, paštrnak i komorač, kao i brojne i često upotrebljavane začinske biljke, kao što su anis, kim, mirođija, korijander i druge. Ove biljke napadaju različite vrste fitopatogenih gljiva iz roda Alternaria, pri čemu je u ovom radu opisano devet vrsta. Međutim, još uvijek je nedovoljno informacija kako o ovim vrstama, tako i o brojnim interakcijama između njih i njihovih biljaka domaćina i faktora spoljne sredine. Ove informacije su neophodne kao osnova za preduzimanje adekvatnih mjera za njihovo suzbijanje i omogućavanje uspješnog i profitabilnog uzgoja ovih na tržištu izuzetno traženih i cijenjenih biljnih vrsta. Zbog toga su u ovom radu prikazane vrste roda Alternaria prourokovači bolesti povrtarskih i začinskih biljaka iz familije Apiaceae, pre svega A. dauci, A. radicina, A. carotiincultae, A. petroselini, A. selini, A. smyrnii, A. alternata, A. longipes i A. burnsii, te njihovi puni aktuelni nazivi i sinonimi, taksonomska pozicija, rasprostranjenost i simptomi koje prouzrokuju na biljkama domaćinima, kao i njihova biologija i način prenošenja i širenja, uz poseban naglasak na mjere za njihovo suzbijanje.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Species of the genus Alternaria pathogens of vegetable and spice plants from the family Apiaceae
T1  - Vrste roda Alternaria patogeni povrtarskih i začinskih biljaka iz familije Apiaceae
EP  - 609
IS  - 6
SP  - 586
VL  - 48
DO  - 10.5937/BiljLek2006586T
ER  - 

@article{
author = "Trkulja, Vojislav and Perviz, Mehira and Mitrović, Petar and Vojvodić, Mira and Bulajić, Aleksandra",
year = "2020",
abstract = "Species of the genus Alternaria are among the most important plant pathogens that occur worldwide on many host plants, as well as plants of the Apiaceae family, including important vegetables such as carrots, parsley, celery, parsnips and fennel, and popular herbs such as anise, cumin, dill, coriander and others. These plants are attacked by different species of phytopathogenic fungi of the genus Alternaria, and this paper offers current review of nine different species. However, there is still insufficient information on both these species and the nu-merous interactions between them and their host plants and environmental factors. This information are necessary as the basis for adequate disease control measures and thus enabling successful and profitable cultivation of these high valued crops. Therefore, we present pathogen profiles of A. dauci, A. radicina, A. carotiincultae, A. petroselini, A. selini, A. smyrnii, A. alternata, A. longipes and A. burnsii as pathogens of Apiaceae plants, as well as their full current names and synonyms, taxonomic position, distribution and symptoms these pathogens cause to their host plants, as well as their biology and mode of transmission and spread, with special emphasis on control measures., Vrste roda Alternaria spadaju među najvažnije biljne patogene koji se pojavljuju širom svijeta na mnogim biljkama domaćinima, uključujući i biljke iz familije Apiaceae. Među njima posebno su značajne povrtrarske biljke kao što su mrkva, peršun, celer, paštrnak i komorač, kao i brojne i često upotrebljavane začinske biljke, kao što su anis, kim, mirođija, korijander i druge. Ove biljke napadaju različite vrste fitopatogenih gljiva iz roda Alternaria, pri čemu je u ovom radu opisano devet vrsta. Međutim, još uvijek je nedovoljno informacija kako o ovim vrstama, tako i o brojnim interakcijama između njih i njihovih biljaka domaćina i faktora spoljne sredine. Ove informacije su neophodne kao osnova za preduzimanje adekvatnih mjera za njihovo suzbijanje i omogućavanje uspješnog i profitabilnog uzgoja ovih na tržištu izuzetno traženih i cijenjenih biljnih vrsta. Zbog toga su u ovom radu prikazane vrste roda Alternaria prourokovači bolesti povrtarskih i začinskih biljaka iz familije Apiaceae, pre svega A. dauci, A. radicina, A. carotiincultae, A. petroselini, A. selini, A. smyrnii, A. alternata, A. longipes i A. burnsii, te njihovi puni aktuelni nazivi i sinonimi, taksonomska pozicija, rasprostranjenost i simptomi koje prouzrokuju na biljkama domaćinima, kao i njihova biologija i način prenošenja i širenja, uz poseban naglasak na mjere za njihovo suzbijanje.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Species of the genus Alternaria pathogens of vegetable and spice plants from the family Apiaceae, Vrste roda Alternaria patogeni povrtarskih i začinskih biljaka iz familije Apiaceae",
pages = "609-586",
number = "6",
volume = "48",
doi = "10.5937/BiljLek2006586T"
}

Trkulja, V., Perviz, M., Mitrović, P., Vojvodić, M.,& Bulajić, A.. (2020). Species of the genus Alternaria pathogens of vegetable and spice plants from the family Apiaceae. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 48(6), 586-609.
https://doi.org/10.5937/BiljLek2006586T

Trkulja V, Perviz M, Mitrović P, Vojvodić M, Bulajić A. Species of the genus Alternaria pathogens of vegetable and spice plants from the family Apiaceae. in Biljni lekar. 2020;48(6):586-609.
doi:10.5937/BiljLek2006586T .

Trkulja, Vojislav, Perviz, Mehira, Mitrović, Petar, Vojvodić, Mira, Bulajić, Aleksandra, "Species of the genus Alternaria pathogens of vegetable and spice plants from the family Apiaceae" in Biljni lekar, 48, no. 6 (2020):586-609,
https://doi.org/10.5937/BiljLek2006586T . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1904
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1759
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}

Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V

Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .

Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .