@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Vuković, S. and Jakšić, Snežana",
year = "2012",
abstract = "Chlorpyrifos is one of the most frequently used insecticide worldwide
and it is more often detected in surface and ground waters compared to other
organophosphorus insecticides. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
In this study a solid phase extraction method was developed for
determination of chlorpyrifos in surface and ground water, which are used for
irrigation in agriculture production,
Samples were prepared by spiking in 500 ml of water (surface and
ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was
carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to
extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50,
v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc
with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated
till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and
homogenised with ultrasound.
Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S,
30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul.
Determination conditions were — column temperature 190°C, increase of
30°C/min, final temperature 275°C; injector temperature 230°C; detector
temperature 300°C.
Linearity of detected response was determined by injecting standard
chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was
0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from
the fortified samples, while the limit of quantification (LOQ) was calculated from
LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average
recoveries of chlorpyrifos detection method in surface water were ranged from
84 % to 89 %, and in groundwater from 87 % to 95 %.",
publisher = "Podgorica : University of Montenegro, Biotechnical Faculty",
journal = "Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro",
title = "Determination of chlorpyrifos in water used for agricultural production",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3314"
}