Histological characteristics of sugar beet leaves potentially linked to drought tolerance
Authorized Users Only
2009
Authors
Luković, JadrankaMaksimović, Ivana
Zorić, Lana
Nagl, Nevena
Percić, Milorad
Polić, Dubravka
Putnik-Delić, Marina
Article (Published version)
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Show full item recordAbstract
Water is becoming more and more limiting factor of sugar beet production and the productivity of the crop can be significantly improved by increased drought tolerance. It is therefore a great challenge to assess the degree of variability of anatomical and morphological traits of breeding material with respect to water use efficiency and drought, that can be used as potential markers for selection of sugar beet genotypes with better tolerance to water shortage. To achieve this, the first step is to assess the degree of genetic variability with respect to anatomical and histological features linked to water management in plants, under optimal water supply. Comparative histiological analysis of lamina and petiole was done on 12 sugar beet genotypes which previously showed divergent responses to lack of water in the field. The plants were grown in semi-controlled conditions of a glasshouse, and watered daily. Mircromorphological analyses were done to assess leaf epidermal characteristics, ...by both light and SEM, and lamina and petiole histological features. The measurements were used to calculate the percentage of individual tissues in relation to the thickness of the lamina, main vein area and petiole area. The general structure of sample variability was established by principal component analysis (PCA), based on correlation matrix. In majority of genotypes the ratio of the size of cells of spongy parenchyma and palisade cells in average was 80%. Low genotypic variability of the studied histological parameters of the lamina and petiole may reflect the narrow genetic base of tested breeding material. The most significant genotypic difference, considering leaf epidermal tissue, was in % of adaxial and abaxial epidermis. The highest found number of stomata per mm(2) on both adaxial and abaxial epidermis was 40% higher than the lowest. During water stress, when stomata are closed, plant survival depends on the amount of water lost through the cuticle. SEM analysis of adaxial epidermis of the lamina show that cuticle varies in texture. Considering the observed genotypic variability in cuticle ornamentation and the fact that plants develop various strategies of adaptation to drought, finding genotypes with increased drought tolerance could be based on the characteristics of the cuticle and epidermis.
Keywords:
Leaf anatomy / Drought / Sugar beet / Cuticle / EpidermisSource:
Industrial Crops and Products, 2009, 30, 2, 281-286Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Poboljšanje germplazme šećerne repe u cilju povećanja prinosa i smanjenja gubitaka nastalih uticajem biotskih i abiotskih faktora (RS-MESTD-MPN2006-2010-20020)
DOI: 10.1016/j.indcrop.2009.05.004
ISSN: 0926-6690
WoS: 000269164400018
Scopus: 2-s2.0-67651177409
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FiVeRTY - JOUR AU - Luković, Jadranka AU - Maksimović, Ivana AU - Zorić, Lana AU - Nagl, Nevena AU - Percić, Milorad AU - Polić, Dubravka AU - Putnik-Delić, Marina PY - 2009 UR - http://fiver.ifvcns.rs/handle/123456789/669 AB - Water is becoming more and more limiting factor of sugar beet production and the productivity of the crop can be significantly improved by increased drought tolerance. It is therefore a great challenge to assess the degree of variability of anatomical and morphological traits of breeding material with respect to water use efficiency and drought, that can be used as potential markers for selection of sugar beet genotypes with better tolerance to water shortage. To achieve this, the first step is to assess the degree of genetic variability with respect to anatomical and histological features linked to water management in plants, under optimal water supply. Comparative histiological analysis of lamina and petiole was done on 12 sugar beet genotypes which previously showed divergent responses to lack of water in the field. The plants were grown in semi-controlled conditions of a glasshouse, and watered daily. Mircromorphological analyses were done to assess leaf epidermal characteristics, by both light and SEM, and lamina and petiole histological features. The measurements were used to calculate the percentage of individual tissues in relation to the thickness of the lamina, main vein area and petiole area. The general structure of sample variability was established by principal component analysis (PCA), based on correlation matrix. In majority of genotypes the ratio of the size of cells of spongy parenchyma and palisade cells in average was 80%. Low genotypic variability of the studied histological parameters of the lamina and petiole may reflect the narrow genetic base of tested breeding material. The most significant genotypic difference, considering leaf epidermal tissue, was in % of adaxial and abaxial epidermis. The highest found number of stomata per mm(2) on both adaxial and abaxial epidermis was 40% higher than the lowest. During water stress, when stomata are closed, plant survival depends on the amount of water lost through the cuticle. SEM analysis of adaxial epidermis of the lamina show that cuticle varies in texture. Considering the observed genotypic variability in cuticle ornamentation and the fact that plants develop various strategies of adaptation to drought, finding genotypes with increased drought tolerance could be based on the characteristics of the cuticle and epidermis. PB - Elsevier, Amsterdam T2 - Industrial Crops and Products T1 - Histological characteristics of sugar beet leaves potentially linked to drought tolerance EP - 286 IS - 2 SP - 281 VL - 30 DO - 10.1016/j.indcrop.2009.05.004 ER -
@article{ author = "Luković, Jadranka and Maksimović, Ivana and Zorić, Lana and Nagl, Nevena and Percić, Milorad and Polić, Dubravka and Putnik-Delić, Marina", year = "2009", abstract = "Water is becoming more and more limiting factor of sugar beet production and the productivity of the crop can be significantly improved by increased drought tolerance. It is therefore a great challenge to assess the degree of variability of anatomical and morphological traits of breeding material with respect to water use efficiency and drought, that can be used as potential markers for selection of sugar beet genotypes with better tolerance to water shortage. To achieve this, the first step is to assess the degree of genetic variability with respect to anatomical and histological features linked to water management in plants, under optimal water supply. Comparative histiological analysis of lamina and petiole was done on 12 sugar beet genotypes which previously showed divergent responses to lack of water in the field. The plants were grown in semi-controlled conditions of a glasshouse, and watered daily. Mircromorphological analyses were done to assess leaf epidermal characteristics, by both light and SEM, and lamina and petiole histological features. The measurements were used to calculate the percentage of individual tissues in relation to the thickness of the lamina, main vein area and petiole area. The general structure of sample variability was established by principal component analysis (PCA), based on correlation matrix. In majority of genotypes the ratio of the size of cells of spongy parenchyma and palisade cells in average was 80%. Low genotypic variability of the studied histological parameters of the lamina and petiole may reflect the narrow genetic base of tested breeding material. The most significant genotypic difference, considering leaf epidermal tissue, was in % of adaxial and abaxial epidermis. The highest found number of stomata per mm(2) on both adaxial and abaxial epidermis was 40% higher than the lowest. During water stress, when stomata are closed, plant survival depends on the amount of water lost through the cuticle. SEM analysis of adaxial epidermis of the lamina show that cuticle varies in texture. Considering the observed genotypic variability in cuticle ornamentation and the fact that plants develop various strategies of adaptation to drought, finding genotypes with increased drought tolerance could be based on the characteristics of the cuticle and epidermis.", publisher = "Elsevier, Amsterdam", journal = "Industrial Crops and Products", title = "Histological characteristics of sugar beet leaves potentially linked to drought tolerance", pages = "286-281", number = "2", volume = "30", doi = "10.1016/j.indcrop.2009.05.004" }
Luković, J., Maksimović, I., Zorić, L., Nagl, N., Percić, M., Polić, D.,& Putnik-Delić, M.. (2009). Histological characteristics of sugar beet leaves potentially linked to drought tolerance. in Industrial Crops and Products Elsevier, Amsterdam., 30(2), 281-286. https://doi.org/10.1016/j.indcrop.2009.05.004
Luković J, Maksimović I, Zorić L, Nagl N, Percić M, Polić D, Putnik-Delić M. Histological characteristics of sugar beet leaves potentially linked to drought tolerance. in Industrial Crops and Products. 2009;30(2):281-286. doi:10.1016/j.indcrop.2009.05.004 .
Luković, Jadranka, Maksimović, Ivana, Zorić, Lana, Nagl, Nevena, Percić, Milorad, Polić, Dubravka, Putnik-Delić, Marina, "Histological characteristics of sugar beet leaves potentially linked to drought tolerance" in Industrial Crops and Products, 30, no. 2 (2009):281-286, https://doi.org/10.1016/j.indcrop.2009.05.004 . .