The appropriate technique for collecting and measuring the amount of floral nectar in sunflower (Helianthus annuus L.)
Конференцијски прилог (Објављена верзија)
Метаподаци
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The available techniques for collecting and measuring the amount of floral nectar are applicable but often found to be unrepresentative. Centrifugation yields larger samples but they also include nectar that is not actually accessible to insects, the capillary method has been described as unsuitable because of possible damage to the nectary tissue, the method including filter paper is considered to be unreliable because of evaporation and nectar extraction methods including washing are considered limited because the solution may include sugars from plant tissue cells. We have found that capillary tubes with inner diameter of 0.25-0.5mm and outer diameter of 0.5-0.75mm are suitable for nectar collection in sunflower. To determine the amount of nectar, we isolate five inflorescences per sunflower line at the start of flowering and collect the nectar two days after the isolation, The capillary tube is inserted between the style and filaments down to the nectary. After the level of nectar ...stops rising the next flower is processed. The tubes can be measured on an analytical scale and the amount of nectar is obtained as the weight increase in comparison to the empty tube. Faster determination of floral nectar amount can be provided by using calibrated capillary tubes of a known and uniform inner diameter. The appropriate outer diameter of the capillary tubes reduces the risk of tissue damage and allows more precise collecting so that the capillary method is preferable to others for nectar collecting in sunflower.
Кључне речи:
capillary technique / nectar quantity / sunflower / Helianthus annuus / floral nectar / collecting techniquesИзвор:
Proceedings, 17th International Sunflower Conference, Cordoba, 8.12 June 2008, 2008, 1, 265-267Издавач:
- Paris : International Sunflower Association
Колекције
Институција/група
FiVeRTY - CONF AU - Sakač, Zvonimir AU - Terzić, Sreten AU - Miklič, Vladimir PY - 2008 UR - http://fiver.ifvcns.rs/handle/123456789/4452 AB - The available techniques for collecting and measuring the amount of floral nectar are applicable but often found to be unrepresentative. Centrifugation yields larger samples but they also include nectar that is not actually accessible to insects, the capillary method has been described as unsuitable because of possible damage to the nectary tissue, the method including filter paper is considered to be unreliable because of evaporation and nectar extraction methods including washing are considered limited because the solution may include sugars from plant tissue cells. We have found that capillary tubes with inner diameter of 0.25-0.5mm and outer diameter of 0.5-0.75mm are suitable for nectar collection in sunflower. To determine the amount of nectar, we isolate five inflorescences per sunflower line at the start of flowering and collect the nectar two days after the isolation, The capillary tube is inserted between the style and filaments down to the nectary. After the level of nectar stops rising the next flower is processed. The tubes can be measured on an analytical scale and the amount of nectar is obtained as the weight increase in comparison to the empty tube. Faster determination of floral nectar amount can be provided by using calibrated capillary tubes of a known and uniform inner diameter. The appropriate outer diameter of the capillary tubes reduces the risk of tissue damage and allows more precise collecting so that the capillary method is preferable to others for nectar collecting in sunflower. PB - Paris : International Sunflower Association C3 - Proceedings, 17th International Sunflower Conference, Cordoba, 8.12 June 2008 T1 - The appropriate technique for collecting and measuring the amount of floral nectar in sunflower (Helianthus annuus L.) EP - 267 SP - 265 VL - 1 UR - https://hdl.handle.net/21.15107/rcub_fiver_4452 ER -
@conference{ author = "Sakač, Zvonimir and Terzić, Sreten and Miklič, Vladimir", year = "2008", abstract = "The available techniques for collecting and measuring the amount of floral nectar are applicable but often found to be unrepresentative. Centrifugation yields larger samples but they also include nectar that is not actually accessible to insects, the capillary method has been described as unsuitable because of possible damage to the nectary tissue, the method including filter paper is considered to be unreliable because of evaporation and nectar extraction methods including washing are considered limited because the solution may include sugars from plant tissue cells. We have found that capillary tubes with inner diameter of 0.25-0.5mm and outer diameter of 0.5-0.75mm are suitable for nectar collection in sunflower. To determine the amount of nectar, we isolate five inflorescences per sunflower line at the start of flowering and collect the nectar two days after the isolation, The capillary tube is inserted between the style and filaments down to the nectary. After the level of nectar stops rising the next flower is processed. The tubes can be measured on an analytical scale and the amount of nectar is obtained as the weight increase in comparison to the empty tube. Faster determination of floral nectar amount can be provided by using calibrated capillary tubes of a known and uniform inner diameter. The appropriate outer diameter of the capillary tubes reduces the risk of tissue damage and allows more precise collecting so that the capillary method is preferable to others for nectar collecting in sunflower.", publisher = "Paris : International Sunflower Association", journal = "Proceedings, 17th International Sunflower Conference, Cordoba, 8.12 June 2008", title = "The appropriate technique for collecting and measuring the amount of floral nectar in sunflower (Helianthus annuus L.)", pages = "267-265", volume = "1", url = "https://hdl.handle.net/21.15107/rcub_fiver_4452" }
Sakač, Z., Terzić, S.,& Miklič, V.. (2008). The appropriate technique for collecting and measuring the amount of floral nectar in sunflower (Helianthus annuus L.). in Proceedings, 17th International Sunflower Conference, Cordoba, 8.12 June 2008 Paris : International Sunflower Association., 1, 265-267. https://hdl.handle.net/21.15107/rcub_fiver_4452
Sakač Z, Terzić S, Miklič V. The appropriate technique for collecting and measuring the amount of floral nectar in sunflower (Helianthus annuus L.). in Proceedings, 17th International Sunflower Conference, Cordoba, 8.12 June 2008. 2008;1:265-267. https://hdl.handle.net/21.15107/rcub_fiver_4452 .
Sakač, Zvonimir, Terzić, Sreten, Miklič, Vladimir, "The appropriate technique for collecting and measuring the amount of floral nectar in sunflower (Helianthus annuus L.)" in Proceedings, 17th International Sunflower Conference, Cordoba, 8.12 June 2008, 1 (2008):265-267, https://hdl.handle.net/21.15107/rcub_fiver_4452 .