Drought-induced Changes in the Antioxidant System in Pisum sativum L.
Аутори
Petrović, GordanaNikolić, Zorica
Živanović, Tomislav
Vasiljević, Sanja
Milošević, Dragana
Stanisavljević, Nemanja
Samardžić, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined.
Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control.
Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmoti...c stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars.
Кључне речи:
osmotic stress / drought / field pea / antioxidative enzymes / gene expression / Pisum sativumИзвор:
Legume Research, 2023, 46, 11, 1445-1452Издавач:
- Agricultural Research Communication Centre
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Колекције
Институција/група
FiVeRTY - JOUR AU - Petrović, Gordana AU - Nikolić, Zorica AU - Živanović, Tomislav AU - Vasiljević, Sanja AU - Milošević, Dragana AU - Stanisavljević, Nemanja AU - Samardžić, Jelena PY - 2023 UR - http://fiver.ifvcns.rs/handle/123456789/4106 AB - Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined. Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control. Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmotic stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars. PB - Agricultural Research Communication Centre T2 - Legume Research T1 - Drought-induced Changes in the Antioxidant System in Pisum sativum L. EP - 1452 IS - 11 SP - 1445 VL - 46 DO - 10.18805/LRF-755 ER -
@article{ author = "Petrović, Gordana and Nikolić, Zorica and Živanović, Tomislav and Vasiljević, Sanja and Milošević, Dragana and Stanisavljević, Nemanja and Samardžić, Jelena", year = "2023", abstract = "Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined. Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control. Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmotic stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars.", publisher = "Agricultural Research Communication Centre", journal = "Legume Research", title = "Drought-induced Changes in the Antioxidant System in Pisum sativum L.", pages = "1452-1445", number = "11", volume = "46", doi = "10.18805/LRF-755" }
Petrović, G., Nikolić, Z., Živanović, T., Vasiljević, S., Milošević, D., Stanisavljević, N.,& Samardžić, J.. (2023). Drought-induced Changes in the Antioxidant System in Pisum sativum L.. in Legume Research Agricultural Research Communication Centre., 46(11), 1445-1452. https://doi.org/10.18805/LRF-755
Petrović G, Nikolić Z, Živanović T, Vasiljević S, Milošević D, Stanisavljević N, Samardžić J. Drought-induced Changes in the Antioxidant System in Pisum sativum L.. in Legume Research. 2023;46(11):1445-1452. doi:10.18805/LRF-755 .
Petrović, Gordana, Nikolić, Zorica, Živanović, Tomislav, Vasiljević, Sanja, Milošević, Dragana, Stanisavljević, Nemanja, Samardžić, Jelena, "Drought-induced Changes in the Antioxidant System in Pisum sativum L." in Legume Research, 46, no. 11 (2023):1445-1452, https://doi.org/10.18805/LRF-755 . .