Molecular markers and oil quality in different Brassicaceae populations

Abstract

In this paper 25 varietal populations ofB. napus (20 winter and 5 spring type), three populations ofB. oleracea and one population ofB. rapa were investigated. The composition of fatty acids in oil was examined by gas chromatography. Polymorphism of genomic DNA, extracted from frozen leaves of the same plant material, was screened with SSR markers. Genetic distances between examined populations were calculated on the basis of polymorphic markers. Data were statistically analyzed with UPGMA method. The resulting dendrogram revealed relations between examined populations. The results on variability of plant material based on fatty acid composition of oil and differences in genomic DNA are comparable.

U radu je ispitivano 25 sortnih populacija uljane repice (B. napus) i to dvadeset ozimih i pet jarih formi, zatim tri populacije ozimog stočnog kelja (B. oleracea) i jedna populacija B. rapa. Sastav viših masnih kiselina u ulju analiziran je gasnom hromatografijom. Polimorfizam genomske DNK, koja je izolovana iz zamrznutih listova istih populacija, je ispitivan sa SSR markerima. Na osnovu polimorfnih markera izračunate su genetičke dis tance između ispitivanih populacija. Primenom statističke analize klastera (UPGMA) konstruisan je dendrogram koji prikazuje srodnost ispitivanih populacija. Rezultati o varijabilnosti sastava viših masnih kiselina u ulju ispitivanih populacija roda Bras sica su u saglasnosti sa rezultatima dobijenim analizom polimorfizma genomske DNK.