Different sterilization methods for overcoming internal bacterial infection in sunflower seeds

Abstract

During culture of protoplasts in agarose droplets, permanent problem was bacterial infection. It was assumed that the seeds are the origin of infection, so different sterilization methods were tested in order to overcome this problem. Germination, infection of seeds and hypocotyls and their growth were examined. Based on these parameters, the best result was obtained with the combined use of 5% commercial bleach and dry heating at 45°C.

Prilikom kultivacije protoplasta gajenog suncokreta u kapljicama agaroze stalan problem je bila bakterijska infekcija. Kako je pretpostavljeno da je seme izvor ove infekcije, isprobane su različite metode njegove sterilizacije da bi se pokušao prevazići ovaj problem. Praćeni su klijavost semena, broj infekcija semena i hipokotila, kao i njihov rast. Na osnovu ovih parametara najbolji rezultat je dobijen nakon kombinovane upotrebe 5% varikine i suve sterilizacije semena na 45°C.