The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers
Authors
Miladinović, DraganaKovačević, Branislav
Jocić, Siniša
Imerovski, Ivana
Dimitrijević, Aleksandra
Cvejić, Sandra
Balalić, Igor
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The success of anther culture depends on numerous factors such as genotype, donor plant growing conditions, anther pre-treatment and development stage, as well as incubation conditions. We have investigated the effect of sowing date and sowing density of donor plants on callus induction and shoot regeneration from cultivated sunflower anthers. Anthers were collected from of three commercial sunflower hybrids that were sown in four different sowing dates, and at three different sowing densities. Anthers were surface sterilized and placed on MS-medium based solid regeneration media. The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analysed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA. Statistical analysis enabled us to determine effect of sowing date and density on anther culture an...d shoot regeneration induction. Sowing date had a significant effect on all observed parameters, with earlier sowing dates having significant positive effect on shoot regeneration. Sowing density had no effect on either of observed parameters in all tested genotypes. The obtained results will contribute to the better understanding of the conditions needed for haploid production in sunflower and its introduction in sunflower breeding programs.
Keywords:
anther culture / dihaploid / donor plant / regeneration / sunflower / sowing date / callus induction / shoot regeneration / anthersSource:
Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey, 2016, 509-514Publisher:
- Paris : International Sunflower Association
Funding / projects:
- Development of new varieties and production technology improvement of oil crops for different purposes (RS-MESTD-Technological Development (TD or TR)-31025)
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FiVeRTY - CONF AU - Miladinović, Dragana AU - Kovačević, Branislav AU - Jocić, Siniša AU - Imerovski, Ivana AU - Dimitrijević, Aleksandra AU - Cvejić, Sandra AU - Balalić, Igor PY - 2016 UR - http://fiver.ifvcns.rs/handle/123456789/2790 AB - The success of anther culture depends on numerous factors such as genotype, donor plant growing conditions, anther pre-treatment and development stage, as well as incubation conditions. We have investigated the effect of sowing date and sowing density of donor plants on callus induction and shoot regeneration from cultivated sunflower anthers. Anthers were collected from of three commercial sunflower hybrids that were sown in four different sowing dates, and at three different sowing densities. Anthers were surface sterilized and placed on MS-medium based solid regeneration media. The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analysed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA. Statistical analysis enabled us to determine effect of sowing date and density on anther culture and shoot regeneration induction. Sowing date had a significant effect on all observed parameters, with earlier sowing dates having significant positive effect on shoot regeneration. Sowing density had no effect on either of observed parameters in all tested genotypes. The obtained results will contribute to the better understanding of the conditions needed for haploid production in sunflower and its introduction in sunflower breeding programs. PB - Paris : International Sunflower Association C3 - Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey T1 - The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers EP - 514 SP - 509 UR - https://hdl.handle.net/21.15107/rcub_fiver_2790 ER -
@conference{ author = "Miladinović, Dragana and Kovačević, Branislav and Jocić, Siniša and Imerovski, Ivana and Dimitrijević, Aleksandra and Cvejić, Sandra and Balalić, Igor", year = "2016", abstract = "The success of anther culture depends on numerous factors such as genotype, donor plant growing conditions, anther pre-treatment and development stage, as well as incubation conditions. We have investigated the effect of sowing date and sowing density of donor plants on callus induction and shoot regeneration from cultivated sunflower anthers. Anthers were collected from of three commercial sunflower hybrids that were sown in four different sowing dates, and at three different sowing densities. Anthers were surface sterilized and placed on MS-medium based solid regeneration media. The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analysed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA. Statistical analysis enabled us to determine effect of sowing date and density on anther culture and shoot regeneration induction. Sowing date had a significant effect on all observed parameters, with earlier sowing dates having significant positive effect on shoot regeneration. Sowing density had no effect on either of observed parameters in all tested genotypes. The obtained results will contribute to the better understanding of the conditions needed for haploid production in sunflower and its introduction in sunflower breeding programs.", publisher = "Paris : International Sunflower Association", journal = "Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey", title = "The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers", pages = "514-509", url = "https://hdl.handle.net/21.15107/rcub_fiver_2790" }
Miladinović, D., Kovačević, B., Jocić, S., Imerovski, I., Dimitrijević, A., Cvejić, S.,& Balalić, I.. (2016). The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey Paris : International Sunflower Association., 509-514. https://hdl.handle.net/21.15107/rcub_fiver_2790
Miladinović D, Kovačević B, Jocić S, Imerovski I, Dimitrijević A, Cvejić S, Balalić I. The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey. 2016;:509-514. https://hdl.handle.net/21.15107/rcub_fiver_2790 .
Miladinović, Dragana, Kovačević, Branislav, Jocić, Siniša, Imerovski, Ivana, Dimitrijević, Aleksandra, Cvejić, Sandra, Balalić, Igor, "The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers" in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey (2016):509-514, https://hdl.handle.net/21.15107/rcub_fiver_2790 .