Lyophilization as a method for pathogens long term preservation
Liofilizacija kao metod dugoročnog čuvanja patogena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Lyophilization (freeze-drying) is one of the most suitable methods used for a long term preservation of pathogens. The aim of this paper was the application of lyophilization for storage of three significant plant pathogens: Fusarium graminearum, Helminthosporium gramineum, and Pseudomonas syringae pv. gylicinea, respectively. The plant material was collected continuously (during a four year period 2002-2006), depending on a plant development stage, from different localities in Vojvodina. Pathogens were isolated from diseased parts with characteristic symptoms, and placed on nutritive media specific for a certain pathogen, using standard phytopathological methods. Lyophilization was carried out in marked and coded ampoules by freezing and drying of pathogen suspension and nutritive medium. Revitalization of lyophilized isolates was done after four days. High percentage of revitalization was characteristic for all studied isolates, and it ranged from 85-92%, confirming that lyophilized ...pathogens would be capable of keeping viability for a long time in the collection. Besides above-mentioned pathogens, there were 200 isolates in the collection, originating mostly from field and vegetable crops. Each isolate that was put into the Collection, was followed by all the necessary data such as: name of the pathogen, number of isolates, locality, host plant, year of isolation, name of the researcher and other relevant data.
Liofilizacija je jedna od najpogodnijih metoda koja se koristi za dugoročno čuvanje patogena. Cilj rada je primena metoda liofilizacije u čuvanju tri značajna biljna patogena: Fusarium graminearum, Helminthosporium gramineum i Pseudomonas syringae pv. gylicinea. Prikupljanje uzoraka biljnog materijala vršeno je u kontinuitetu (tokom četiri godine, 2002-2006) sa različitih lokaliteta na području Vojvodine, u zavisnosti od razvojne faze biljaka. Izolacija patogena vršena je iz obolelih biljnih delova sa karakterističnim simptomima na hranljive podloge specifične za određenog patogena korišćenjem standardnih fitopatoloških metoda.
Кључне речи:
lyophilization / long time preservation / plant pathogen / collection / fungi / bacteria / pathogens / storage / liofilizacija / dugoročno čuvanje / patogeni / gljive / bakterije / čuvanje / izolati / isolatesИзвор:
Zbornik Matice srpske za prirodne nauke, 2007, 113, 203-210Издавач:
- Novi Sad : Matica srpska
Колекције
Институција/група
FiVeRTY - JOUR AU - Milošević, Mirjana AU - Medić-Pap, Slađana AU - Ignjatov, Maja AU - Milošević, Dragana PY - 2007 UR - http://fiver.ifvcns.rs/handle/123456789/2641 AB - Lyophilization (freeze-drying) is one of the most suitable methods used for a long term preservation of pathogens. The aim of this paper was the application of lyophilization for storage of three significant plant pathogens: Fusarium graminearum, Helminthosporium gramineum, and Pseudomonas syringae pv. gylicinea, respectively. The plant material was collected continuously (during a four year period 2002-2006), depending on a plant development stage, from different localities in Vojvodina. Pathogens were isolated from diseased parts with characteristic symptoms, and placed on nutritive media specific for a certain pathogen, using standard phytopathological methods. Lyophilization was carried out in marked and coded ampoules by freezing and drying of pathogen suspension and nutritive medium. Revitalization of lyophilized isolates was done after four days. High percentage of revitalization was characteristic for all studied isolates, and it ranged from 85-92%, confirming that lyophilized pathogens would be capable of keeping viability for a long time in the collection. Besides above-mentioned pathogens, there were 200 isolates in the collection, originating mostly from field and vegetable crops. Each isolate that was put into the Collection, was followed by all the necessary data such as: name of the pathogen, number of isolates, locality, host plant, year of isolation, name of the researcher and other relevant data. AB - Liofilizacija je jedna od najpogodnijih metoda koja se koristi za dugoročno čuvanje patogena. Cilj rada je primena metoda liofilizacije u čuvanju tri značajna biljna patogena: Fusarium graminearum, Helminthosporium gramineum i Pseudomonas syringae pv. gylicinea. Prikupljanje uzoraka biljnog materijala vršeno je u kontinuitetu (tokom četiri godine, 2002-2006) sa različitih lokaliteta na području Vojvodine, u zavisnosti od razvojne faze biljaka. Izolacija patogena vršena je iz obolelih biljnih delova sa karakterističnim simptomima na hranljive podloge specifične za određenog patogena korišćenjem standardnih fitopatoloških metoda. PB - Novi Sad : Matica srpska T2 - Zbornik Matice srpske za prirodne nauke T1 - Lyophilization as a method for pathogens long term preservation T1 - Liofilizacija kao metod dugoročnog čuvanja patogena EP - 210 SP - 203 VL - 113 UR - https://hdl.handle.net/21.15107/rcub_fiver_2641 ER -
@article{ author = "Milošević, Mirjana and Medić-Pap, Slađana and Ignjatov, Maja and Milošević, Dragana", year = "2007", abstract = "Lyophilization (freeze-drying) is one of the most suitable methods used for a long term preservation of pathogens. The aim of this paper was the application of lyophilization for storage of three significant plant pathogens: Fusarium graminearum, Helminthosporium gramineum, and Pseudomonas syringae pv. gylicinea, respectively. The plant material was collected continuously (during a four year period 2002-2006), depending on a plant development stage, from different localities in Vojvodina. Pathogens were isolated from diseased parts with characteristic symptoms, and placed on nutritive media specific for a certain pathogen, using standard phytopathological methods. Lyophilization was carried out in marked and coded ampoules by freezing and drying of pathogen suspension and nutritive medium. Revitalization of lyophilized isolates was done after four days. High percentage of revitalization was characteristic for all studied isolates, and it ranged from 85-92%, confirming that lyophilized pathogens would be capable of keeping viability for a long time in the collection. Besides above-mentioned pathogens, there were 200 isolates in the collection, originating mostly from field and vegetable crops. Each isolate that was put into the Collection, was followed by all the necessary data such as: name of the pathogen, number of isolates, locality, host plant, year of isolation, name of the researcher and other relevant data., Liofilizacija je jedna od najpogodnijih metoda koja se koristi za dugoročno čuvanje patogena. Cilj rada je primena metoda liofilizacije u čuvanju tri značajna biljna patogena: Fusarium graminearum, Helminthosporium gramineum i Pseudomonas syringae pv. gylicinea. Prikupljanje uzoraka biljnog materijala vršeno je u kontinuitetu (tokom četiri godine, 2002-2006) sa različitih lokaliteta na području Vojvodine, u zavisnosti od razvojne faze biljaka. Izolacija patogena vršena je iz obolelih biljnih delova sa karakterističnim simptomima na hranljive podloge specifične za određenog patogena korišćenjem standardnih fitopatoloških metoda.", publisher = "Novi Sad : Matica srpska", journal = "Zbornik Matice srpske za prirodne nauke", title = "Lyophilization as a method for pathogens long term preservation, Liofilizacija kao metod dugoročnog čuvanja patogena", pages = "210-203", volume = "113", url = "https://hdl.handle.net/21.15107/rcub_fiver_2641" }
Milošević, M., Medić-Pap, S., Ignjatov, M.,& Milošević, D.. (2007). Lyophilization as a method for pathogens long term preservation. in Zbornik Matice srpske za prirodne nauke Novi Sad : Matica srpska., 113, 203-210. https://hdl.handle.net/21.15107/rcub_fiver_2641
Milošević M, Medić-Pap S, Ignjatov M, Milošević D. Lyophilization as a method for pathogens long term preservation. in Zbornik Matice srpske za prirodne nauke. 2007;113:203-210. https://hdl.handle.net/21.15107/rcub_fiver_2641 .
Milošević, Mirjana, Medić-Pap, Slađana, Ignjatov, Maja, Milošević, Dragana, "Lyophilization as a method for pathogens long term preservation" in Zbornik Matice srpske za prirodne nauke, 113 (2007):203-210, https://hdl.handle.net/21.15107/rcub_fiver_2641 .