Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The bacterium Pseudomonas syringae pv. tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Eu...rope Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.
Кључне речи:
Pseudomonas syringae pv. tomato / diseases / tomato / Serbia / seedsИзвор:
Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007, 2007, 135-135Издавач:
- Leuven : International Society for Horticultural Science
Колекције
Институција/група
FiVeRTY - CONF AU - Ignjatov, Maja AU - Milošević, Mirjana AU - Petrović, Dragana AU - Medić-Pap, Slađana PY - 2007 UR - http://fiver.ifvcns.rs/handle/123456789/2605 AB - The bacterium Pseudomonas syringae pv. tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Europe Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds. PB - Leuven : International Society for Horticultural Science C3 - Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007 T1 - Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds EP - 135 SP - 135 UR - https://hdl.handle.net/21.15107/rcub_fiver_2605 ER -
@conference{ author = "Ignjatov, Maja and Milošević, Mirjana and Petrović, Dragana and Medić-Pap, Slađana", year = "2007", abstract = "The bacterium Pseudomonas syringae pv. tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Europe Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.", publisher = "Leuven : International Society for Horticultural Science", journal = "Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007", title = "Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds", pages = "135-135", url = "https://hdl.handle.net/21.15107/rcub_fiver_2605" }
Ignjatov, M., Milošević, M., Petrović, D.,& Medić-Pap, S.. (2007). Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007 Leuven : International Society for Horticultural Science., 135-135. https://hdl.handle.net/21.15107/rcub_fiver_2605
Ignjatov M, Milošević M, Petrović D, Medić-Pap S. Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007. 2007;:135-135. https://hdl.handle.net/21.15107/rcub_fiver_2605 .
Ignjatov, Maja, Milošević, Mirjana, Petrović, Dragana, Medić-Pap, Slađana, "Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds" in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007 (2007):135-135, https://hdl.handle.net/21.15107/rcub_fiver_2605 .