The applicability of species- and trichothecene-specific primers in monitoring the Fusarium graminearum species complex and its impact on the surveillance of fusarium head blight in winter wheat in Serbia
Апстракт
Monitoring changes in the prevalence of Fusarium species and toxin production is an important tool for the integrated control of Fusarium head blight (FHB). However, methods for the high-throughput screening of Fusarium populations have been developed using isolates with limited geographic origins. In this study, we used species- and trichothecene-specific primers to monitor the F. graminearum species complex (FGSC) originating from Serbia. We also tested the applicability of the primers to the surveillance of FHB. We analyzed two hundred and ten isolates collected from thirty two locations and five winter wheat varieties over a three-year period. Using multiple correspondence analysis (MCA), we investigated associations between Fusarium-damaged kernels (FDK) and location, variety, members of the FGSC, and their predisposition for mycotoxin production. The results revealed that the species-specific primers were not specific for 11% of the F. graminearum population. The primer sets were... 98.5%, 95.2%, and 92.4% effective in the multilocus genotyping of Tri7, Tri3, and Tri5 genes, respectively. We found that individual wheat varieties were associated with isolates that could not be characterized using species- and trichothecene-specific primers. Alternaria spp. had a significant influence (p < 0.001) on grain infection with F. graminearum, indicating the necessity to further investigate its impact on the pathogenesis of the F. graminearum clade.
Кључне речи:
winter wheat / trichothecene genotyping / grain infection / FGSC complex / Fusarium Head BlightИзвор:
Agronomy, 2021, 11, 778-Издавач:
- Basel : MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
DOI: 10.3390/agronomy11040778
ISSN: 2073-4395
WoS: 000642695200001
Scopus: 2-s2.0-85106714497
Колекције
Институција/група
FiVeRTY - JOUR AU - Župunski, Vesna AU - Lalošević, Mirjana AU - Mikić, Sanja AU - Orbović, Branka AU - Jevtić, Radivoje PY - 2021 UR - http://fiver.ifvcns.rs/handle/123456789/2240 AB - Monitoring changes in the prevalence of Fusarium species and toxin production is an important tool for the integrated control of Fusarium head blight (FHB). However, methods for the high-throughput screening of Fusarium populations have been developed using isolates with limited geographic origins. In this study, we used species- and trichothecene-specific primers to monitor the F. graminearum species complex (FGSC) originating from Serbia. We also tested the applicability of the primers to the surveillance of FHB. We analyzed two hundred and ten isolates collected from thirty two locations and five winter wheat varieties over a three-year period. Using multiple correspondence analysis (MCA), we investigated associations between Fusarium-damaged kernels (FDK) and location, variety, members of the FGSC, and their predisposition for mycotoxin production. The results revealed that the species-specific primers were not specific for 11% of the F. graminearum population. The primer sets were 98.5%, 95.2%, and 92.4% effective in the multilocus genotyping of Tri7, Tri3, and Tri5 genes, respectively. We found that individual wheat varieties were associated with isolates that could not be characterized using species- and trichothecene-specific primers. Alternaria spp. had a significant influence (p < 0.001) on grain infection with F. graminearum, indicating the necessity to further investigate its impact on the pathogenesis of the F. graminearum clade. PB - Basel : MDPI T2 - Agronomy T1 - The applicability of species- and trichothecene-specific primers in monitoring the Fusarium graminearum species complex and its impact on the surveillance of fusarium head blight in winter wheat in Serbia SP - 778 VL - 11 DO - 10.3390/agronomy11040778 ER -
@article{ author = "Župunski, Vesna and Lalošević, Mirjana and Mikić, Sanja and Orbović, Branka and Jevtić, Radivoje", year = "2021", abstract = "Monitoring changes in the prevalence of Fusarium species and toxin production is an important tool for the integrated control of Fusarium head blight (FHB). However, methods for the high-throughput screening of Fusarium populations have been developed using isolates with limited geographic origins. In this study, we used species- and trichothecene-specific primers to monitor the F. graminearum species complex (FGSC) originating from Serbia. We also tested the applicability of the primers to the surveillance of FHB. We analyzed two hundred and ten isolates collected from thirty two locations and five winter wheat varieties over a three-year period. Using multiple correspondence analysis (MCA), we investigated associations between Fusarium-damaged kernels (FDK) and location, variety, members of the FGSC, and their predisposition for mycotoxin production. The results revealed that the species-specific primers were not specific for 11% of the F. graminearum population. The primer sets were 98.5%, 95.2%, and 92.4% effective in the multilocus genotyping of Tri7, Tri3, and Tri5 genes, respectively. We found that individual wheat varieties were associated with isolates that could not be characterized using species- and trichothecene-specific primers. Alternaria spp. had a significant influence (p < 0.001) on grain infection with F. graminearum, indicating the necessity to further investigate its impact on the pathogenesis of the F. graminearum clade.", publisher = "Basel : MDPI", journal = "Agronomy", title = "The applicability of species- and trichothecene-specific primers in monitoring the Fusarium graminearum species complex and its impact on the surveillance of fusarium head blight in winter wheat in Serbia", pages = "778", volume = "11", doi = "10.3390/agronomy11040778" }
Župunski, V., Lalošević, M., Mikić, S., Orbović, B.,& Jevtić, R.. (2021). The applicability of species- and trichothecene-specific primers in monitoring the Fusarium graminearum species complex and its impact on the surveillance of fusarium head blight in winter wheat in Serbia. in Agronomy Basel : MDPI., 11, 778. https://doi.org/10.3390/agronomy11040778
Župunski V, Lalošević M, Mikić S, Orbović B, Jevtić R. The applicability of species- and trichothecene-specific primers in monitoring the Fusarium graminearum species complex and its impact on the surveillance of fusarium head blight in winter wheat in Serbia. in Agronomy. 2021;11:778. doi:10.3390/agronomy11040778 .
Župunski, Vesna, Lalošević, Mirjana, Mikić, Sanja, Orbović, Branka, Jevtić, Radivoje, "The applicability of species- and trichothecene-specific primers in monitoring the Fusarium graminearum species complex and its impact on the surveillance of fusarium head blight in winter wheat in Serbia" in Agronomy, 11 (2021):778, https://doi.org/10.3390/agronomy11040778 . .