Effect of cadmium on callus growth in wheat zygotic embryo culture
Uticaj kadmijuma na razviće kalusa u kulturi zigotnog embriona pšenice
Abstract
Toxicity effects of cadmium on callus growth and callus tissue dry matter content were investigated. Two winter wheat varieties, Evropa-90 and NSR-5 were used as a material in embryo culture. Isolated embryos were cultivated on a modified MS (Murashige and Skoog, 1962) medium to which Cd was added in three different concentrations (10-6, 10-5 and 10-4 M/l Cd). At the highest Cd concentration (10-4 m/l), CV. evropa-90 had 90.3% and CV. nsr-5 52.5% of survived calluses. Fresh callus weight was reduced at the concentration of 10-4 M/l Cd by 51% in cv. Evropa-90 and by 57% in cv. NSR-5 in relation to the control. Cadmium decreased the callus dry matter content in cv. NSR-5 only at the highest concentration (10-4 M/l), while in cv. Evropa-90 Cd had no signifficant effect on callus dry matter content.
Ispitivano je toksično dejstvo kadmijuma na porast i sadržaj suve materije u kalusima pšenice. Dve sorte ozime pšenice, Evropa-90 i NSR-5, korišćene su kao materijal u kulturi embriona. Izolovani embrioni su kultivisani na modifikovanu MS podlogu (Murashige and Skoog, 1962) kojoj je Cd dodat u tri različite koncentracije (10-6, 10-5 i 10-4 M/l Cd). Pri najvišoj koncentraciji Cd (10-4 M/l), sorta Evropa-90 je imala 90.3%, a sorta NSR-5 52.5% preživelih kalusa. Pri koncentraciji od 10-4 M/l CdCl2 sveža masa kalusa je bila umanjena za 51% kod sorte Evropa-90, a kod sorte NSR-5 za 57%. Kadmijum je izazvao sniženje sadržaja suve materije u kalusima sorte NSR-5 samo pri najvišoj koncentraciji (10-4 M/l), dok kod sorte Evropa-90 Cd nije imao značajnog uticaja na sadržaj suve materije u kalusima.
Keywords:
wheat / cultivars / cadmium / embryos / in vitro* / pšenica / sorte / kadmijum / embrioni / in vitroSource:
Zbornik radova Instituta za ratarstvo i povrtarstvo, 2003, 39, 71-75Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
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Institution/Community
FiVeRTY - JOUR AU - Kondić-Špika, Ankica PY - 2003 UR - http://fiver.ifvcns.rs/handle/123456789/209 AB - Toxicity effects of cadmium on callus growth and callus tissue dry matter content were investigated. Two winter wheat varieties, Evropa-90 and NSR-5 were used as a material in embryo culture. Isolated embryos were cultivated on a modified MS (Murashige and Skoog, 1962) medium to which Cd was added in three different concentrations (10-6, 10-5 and 10-4 M/l Cd). At the highest Cd concentration (10-4 m/l), CV. evropa-90 had 90.3% and CV. nsr-5 52.5% of survived calluses. Fresh callus weight was reduced at the concentration of 10-4 M/l Cd by 51% in cv. Evropa-90 and by 57% in cv. NSR-5 in relation to the control. Cadmium decreased the callus dry matter content in cv. NSR-5 only at the highest concentration (10-4 M/l), while in cv. Evropa-90 Cd had no signifficant effect on callus dry matter content. AB - Ispitivano je toksično dejstvo kadmijuma na porast i sadržaj suve materije u kalusima pšenice. Dve sorte ozime pšenice, Evropa-90 i NSR-5, korišćene su kao materijal u kulturi embriona. Izolovani embrioni su kultivisani na modifikovanu MS podlogu (Murashige and Skoog, 1962) kojoj je Cd dodat u tri različite koncentracije (10-6, 10-5 i 10-4 M/l Cd). Pri najvišoj koncentraciji Cd (10-4 M/l), sorta Evropa-90 je imala 90.3%, a sorta NSR-5 52.5% preživelih kalusa. Pri koncentraciji od 10-4 M/l CdCl2 sveža masa kalusa je bila umanjena za 51% kod sorte Evropa-90, a kod sorte NSR-5 za 57%. Kadmijum je izazvao sniženje sadržaja suve materije u kalusima sorte NSR-5 samo pri najvišoj koncentraciji (10-4 M/l), dok kod sorte Evropa-90 Cd nije imao značajnog uticaja na sadržaj suve materije u kalusima. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Zbornik radova Instituta za ratarstvo i povrtarstvo T1 - Effect of cadmium on callus growth in wheat zygotic embryo culture T1 - Uticaj kadmijuma na razviće kalusa u kulturi zigotnog embriona pšenice EP - 75 IS - 39 SP - 71 UR - https://hdl.handle.net/21.15107/rcub_fiver_209 ER -
@article{ author = "Kondić-Špika, Ankica", year = "2003", abstract = "Toxicity effects of cadmium on callus growth and callus tissue dry matter content were investigated. Two winter wheat varieties, Evropa-90 and NSR-5 were used as a material in embryo culture. Isolated embryos were cultivated on a modified MS (Murashige and Skoog, 1962) medium to which Cd was added in three different concentrations (10-6, 10-5 and 10-4 M/l Cd). At the highest Cd concentration (10-4 m/l), CV. evropa-90 had 90.3% and CV. nsr-5 52.5% of survived calluses. Fresh callus weight was reduced at the concentration of 10-4 M/l Cd by 51% in cv. Evropa-90 and by 57% in cv. NSR-5 in relation to the control. Cadmium decreased the callus dry matter content in cv. NSR-5 only at the highest concentration (10-4 M/l), while in cv. Evropa-90 Cd had no signifficant effect on callus dry matter content., Ispitivano je toksično dejstvo kadmijuma na porast i sadržaj suve materije u kalusima pšenice. Dve sorte ozime pšenice, Evropa-90 i NSR-5, korišćene su kao materijal u kulturi embriona. Izolovani embrioni su kultivisani na modifikovanu MS podlogu (Murashige and Skoog, 1962) kojoj je Cd dodat u tri različite koncentracije (10-6, 10-5 i 10-4 M/l Cd). Pri najvišoj koncentraciji Cd (10-4 M/l), sorta Evropa-90 je imala 90.3%, a sorta NSR-5 52.5% preživelih kalusa. Pri koncentraciji od 10-4 M/l CdCl2 sveža masa kalusa je bila umanjena za 51% kod sorte Evropa-90, a kod sorte NSR-5 za 57%. Kadmijum je izazvao sniženje sadržaja suve materije u kalusima sorte NSR-5 samo pri najvišoj koncentraciji (10-4 M/l), dok kod sorte Evropa-90 Cd nije imao značajnog uticaja na sadržaj suve materije u kalusima.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo", title = "Effect of cadmium on callus growth in wheat zygotic embryo culture, Uticaj kadmijuma na razviće kalusa u kulturi zigotnog embriona pšenice", pages = "75-71", number = "39", url = "https://hdl.handle.net/21.15107/rcub_fiver_209" }
Kondić-Špika, A.. (2003). Effect of cadmium on callus growth in wheat zygotic embryo culture. in Zbornik radova Instituta za ratarstvo i povrtarstvo Institut za ratarstvo i povrtarstvo, Novi Sad.(39), 71-75. https://hdl.handle.net/21.15107/rcub_fiver_209
Kondić-Špika A. Effect of cadmium on callus growth in wheat zygotic embryo culture. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2003;(39):71-75. https://hdl.handle.net/21.15107/rcub_fiver_209 .
Kondić-Špika, Ankica, "Effect of cadmium on callus growth in wheat zygotic embryo culture" in Zbornik radova Instituta za ratarstvo i povrtarstvo, no. 39 (2003):71-75, https://hdl.handle.net/21.15107/rcub_fiver_209 .