Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity
2020
Аутори
Zheljazkov, Valtcho D.Sikora, Vladimir
Semerdjieva, Ivanka B.
Kacaniova, Miroslava
Astatkie, Tess
Dincheva, Ivayla
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The hypothesis of this study was that we can modify the essential oil (EO) profile of hemp (Cannabis sativaL.) and obtain fractions with differential composition and antimicrobial activity. Therefore, the objective was to evaluate the effects of grinding of hemp biomass before EO extraction and fractionation during distillation on EO profile and antimicrobial activity. The study generated a several EO fractions with a diversity of chemical profile and antimicrobial activity. The highest concentrations of beta-pinene and myrcene in the EO can be obtained in the 5-10 min distillation time (DT) of ground material or in the 80-120 min DT of nonground material. High delta-3-carene and limonene EO can be obtained from 0-5 min DT fraction of nonground material. High eucalyptol EO can be sampled either in the 0-5 min DT of the ground material or in the 80-120 min of nonground material. Overall, the highest concentrations of beta-caryophyllene,alpha-(E)-bergamotene, (Z)-beta-farnesene,alpha-hum...ulene, caryophyllenyl alcohol, germacrene D-4-ol, spathulenol, caryophyllene oxide, humulene epoxide 2,beta-bisabolol,alpha-bisabolol, sesquiterpenes, and cannabidiol (CBD) can be obtained when EO is sampled in the 80-120 min DT and the material is nonground. Monoterpenes in the hemp EO can be increased twofold to 85% by grinding the material prior to distillation and collecting the EO in the first 10 min. However, grinding resulted in a slight but significant decrease in the CBD concentration of the EO. CBD-rich oil can be produced by collecting at 120-180 min DT. Different EO fractions had differential antimicrobial activity. The highest antimicrobial activity of EO fraction was found againstStaphylococcus aureussubsp.aureus. THC-free EO can be obtained if the EO distillation is limited to 120 min. The results can be utilized by the hemp processing industry and by companies developing new hemp EO-infused products, including perfumery, cosmetics, dietary supplements, food, and pharmaceutical industries.
Кључне речи:
Cannabis sativa / volatile oil / cannabinoids / cannabidiol / delta 9-tetrahydrocannabinol / dronabinol / monoterpenes / sesquiterpenes / bioactivityИзвор:
Molecules, 2020, 25, 17Издавач:
- Basel : MDPI
DOI: 10.3390/molecules25173943
ISSN: 1420-3049
PubMed: 32872359
WoS: 000569759000001
Scopus: 2-s2.0-85090180425
Колекције
Институција/група
FiVeRTY - JOUR AU - Zheljazkov, Valtcho D. AU - Sikora, Vladimir AU - Semerdjieva, Ivanka B. AU - Kacaniova, Miroslava AU - Astatkie, Tess AU - Dincheva, Ivayla PY - 2020 UR - http://fiver.ifvcns.rs/handle/123456789/2043 AB - The hypothesis of this study was that we can modify the essential oil (EO) profile of hemp (Cannabis sativaL.) and obtain fractions with differential composition and antimicrobial activity. Therefore, the objective was to evaluate the effects of grinding of hemp biomass before EO extraction and fractionation during distillation on EO profile and antimicrobial activity. The study generated a several EO fractions with a diversity of chemical profile and antimicrobial activity. The highest concentrations of beta-pinene and myrcene in the EO can be obtained in the 5-10 min distillation time (DT) of ground material or in the 80-120 min DT of nonground material. High delta-3-carene and limonene EO can be obtained from 0-5 min DT fraction of nonground material. High eucalyptol EO can be sampled either in the 0-5 min DT of the ground material or in the 80-120 min of nonground material. Overall, the highest concentrations of beta-caryophyllene,alpha-(E)-bergamotene, (Z)-beta-farnesene,alpha-humulene, caryophyllenyl alcohol, germacrene D-4-ol, spathulenol, caryophyllene oxide, humulene epoxide 2,beta-bisabolol,alpha-bisabolol, sesquiterpenes, and cannabidiol (CBD) can be obtained when EO is sampled in the 80-120 min DT and the material is nonground. Monoterpenes in the hemp EO can be increased twofold to 85% by grinding the material prior to distillation and collecting the EO in the first 10 min. However, grinding resulted in a slight but significant decrease in the CBD concentration of the EO. CBD-rich oil can be produced by collecting at 120-180 min DT. Different EO fractions had differential antimicrobial activity. The highest antimicrobial activity of EO fraction was found againstStaphylococcus aureussubsp.aureus. THC-free EO can be obtained if the EO distillation is limited to 120 min. The results can be utilized by the hemp processing industry and by companies developing new hemp EO-infused products, including perfumery, cosmetics, dietary supplements, food, and pharmaceutical industries. PB - Basel : MDPI T2 - Molecules T1 - Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity IS - 17 VL - 25 DO - 10.3390/molecules25173943 ER -
@article{ author = "Zheljazkov, Valtcho D. and Sikora, Vladimir and Semerdjieva, Ivanka B. and Kacaniova, Miroslava and Astatkie, Tess and Dincheva, Ivayla", year = "2020", abstract = "The hypothesis of this study was that we can modify the essential oil (EO) profile of hemp (Cannabis sativaL.) and obtain fractions with differential composition and antimicrobial activity. Therefore, the objective was to evaluate the effects of grinding of hemp biomass before EO extraction and fractionation during distillation on EO profile and antimicrobial activity. The study generated a several EO fractions with a diversity of chemical profile and antimicrobial activity. The highest concentrations of beta-pinene and myrcene in the EO can be obtained in the 5-10 min distillation time (DT) of ground material or in the 80-120 min DT of nonground material. High delta-3-carene and limonene EO can be obtained from 0-5 min DT fraction of nonground material. High eucalyptol EO can be sampled either in the 0-5 min DT of the ground material or in the 80-120 min of nonground material. Overall, the highest concentrations of beta-caryophyllene,alpha-(E)-bergamotene, (Z)-beta-farnesene,alpha-humulene, caryophyllenyl alcohol, germacrene D-4-ol, spathulenol, caryophyllene oxide, humulene epoxide 2,beta-bisabolol,alpha-bisabolol, sesquiterpenes, and cannabidiol (CBD) can be obtained when EO is sampled in the 80-120 min DT and the material is nonground. Monoterpenes in the hemp EO can be increased twofold to 85% by grinding the material prior to distillation and collecting the EO in the first 10 min. However, grinding resulted in a slight but significant decrease in the CBD concentration of the EO. CBD-rich oil can be produced by collecting at 120-180 min DT. Different EO fractions had differential antimicrobial activity. The highest antimicrobial activity of EO fraction was found againstStaphylococcus aureussubsp.aureus. THC-free EO can be obtained if the EO distillation is limited to 120 min. The results can be utilized by the hemp processing industry and by companies developing new hemp EO-infused products, including perfumery, cosmetics, dietary supplements, food, and pharmaceutical industries.", publisher = "Basel : MDPI", journal = "Molecules", title = "Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity", number = "17", volume = "25", doi = "10.3390/molecules25173943" }
Zheljazkov, V. D., Sikora, V., Semerdjieva, I. B., Kacaniova, M., Astatkie, T.,& Dincheva, I.. (2020). Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity. in Molecules Basel : MDPI., 25(17). https://doi.org/10.3390/molecules25173943
Zheljazkov VD, Sikora V, Semerdjieva IB, Kacaniova M, Astatkie T, Dincheva I. Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity. in Molecules. 2020;25(17). doi:10.3390/molecules25173943 .
Zheljazkov, Valtcho D., Sikora, Vladimir, Semerdjieva, Ivanka B., Kacaniova, Miroslava, Astatkie, Tess, Dincheva, Ivayla, "Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity" in Molecules, 25, no. 17 (2020), https://doi.org/10.3390/molecules25173943 . .