BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines
Samo za registrovane korisnike
2019
Autori
Imerovski, IvanaDedić, Boško
Cvejić, Sandra
Miladinović, Dragana
Jocić, Siniša
Owens, Gregory L.
Kočiš-Tubić, Nataša
Rieseberg, Loren H.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Broomrape (Orobanche cumana) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F-3 families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated or3.1 and or3.2. QTL or3.1 was positioned in a genomic region where a previous broomrape resistance gene Or5 has been mapped, while QTL or3.2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F-2 populations were genotyped us...ing newly developed CAPS markers nearest to the QTL peak for or3.2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.
Ključne reči:
Sunflower / Broomrape / QTL / Race G / GBS / BSAIzvor:
Molecular Breeding, 2019, 39, 3Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Razvoj novih sorti i poboljšanje tehnologija proizvodnje uljanih biljnih vrsta za različite namene (RS-MESTD-Technological Development (TD or TR)-31025)
- Genome BC
- Genome CanadaGenome Canada
DOI: 10.1007/s11032-019-0948-9
ISSN: 1380-3743
WoS: 000460161000001
Scopus: 2-s2.0-85062236110
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Imerovski, Ivana AU - Dedić, Boško AU - Cvejić, Sandra AU - Miladinović, Dragana AU - Jocić, Siniša AU - Owens, Gregory L. AU - Kočiš-Tubić, Nataša AU - Rieseberg, Loren H. PY - 2019 UR - http://fiver.ifvcns.rs/handle/123456789/1933 AB - Broomrape (Orobanche cumana) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F-3 families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated or3.1 and or3.2. QTL or3.1 was positioned in a genomic region where a previous broomrape resistance gene Or5 has been mapped, while QTL or3.2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F-2 populations were genotyped using newly developed CAPS markers nearest to the QTL peak for or3.2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs. PB - Springer, Dordrecht T2 - Molecular Breeding T1 - BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines IS - 3 VL - 39 DO - 10.1007/s11032-019-0948-9 ER -
@article{ author = "Imerovski, Ivana and Dedić, Boško and Cvejić, Sandra and Miladinović, Dragana and Jocić, Siniša and Owens, Gregory L. and Kočiš-Tubić, Nataša and Rieseberg, Loren H.", year = "2019", abstract = "Broomrape (Orobanche cumana) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F-3 families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated or3.1 and or3.2. QTL or3.1 was positioned in a genomic region where a previous broomrape resistance gene Or5 has been mapped, while QTL or3.2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F-2 populations were genotyped using newly developed CAPS markers nearest to the QTL peak for or3.2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.", publisher = "Springer, Dordrecht", journal = "Molecular Breeding", title = "BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines", number = "3", volume = "39", doi = "10.1007/s11032-019-0948-9" }
Imerovski, I., Dedić, B., Cvejić, S., Miladinović, D., Jocić, S., Owens, G. L., Kočiš-Tubić, N.,& Rieseberg, L. H.. (2019). BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines. in Molecular Breeding Springer, Dordrecht., 39(3). https://doi.org/10.1007/s11032-019-0948-9
Imerovski I, Dedić B, Cvejić S, Miladinović D, Jocić S, Owens GL, Kočiš-Tubić N, Rieseberg LH. BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines. in Molecular Breeding. 2019;39(3). doi:10.1007/s11032-019-0948-9 .
Imerovski, Ivana, Dedić, Boško, Cvejić, Sandra, Miladinović, Dragana, Jocić, Siniša, Owens, Gregory L., Kočiš-Tubić, Nataša, Rieseberg, Loren H., "BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines" in Molecular Breeding, 39, no. 3 (2019), https://doi.org/10.1007/s11032-019-0948-9 . .