Molecular diversity and microsatellite polymorphism of modern maize hybrids

Abstract

The aim of this study was to estimate genetic diversity of 97 modern maize hybrids cultivated in Serbia with 12 microsatellite markers. In total, 89 alleles were detected, with the average of 7.4 alleles per locus. The polymorphic information content ranged from 0.42, for umc1792, to 0.81, for dupssr10, with an average of 0.64. The pairwise relatedness Ritland's values varied between 0.787 and -0.129. Eight markers were sufficient to differentiate hybrids with unique genotypes. A principal coordinate analysis distinguished early and late maturing groups of hybrids, although the observed differentiation between groups was low (Fst = 0.4%). Two markers, bnlg1556 and umc1075, contributed most to the discrimination between early and late genotypes. The values of parameters of molecular genetic diversity were higher in early than in late maturing hybrids, indicating broader genetic basis of the former. A high level of polymorphism of the markers indicates their suitability for fingerprinting.

Cilj ovog rada bio je da se proceni genetički diverzitet 97 savremenih hibrida kukuruza koji se gaje u Srbiji pomoću 12 mikrosatelitskih markera. Od ukupno 89 alela, prosečno je utvrđeno 7.4 alela po lokusu. Vrednost polimorfnosti pojedinačnih lokusa (PIC) kretala se od 0.42, za marker umc1792, do 0.81, za marker dupssr10, sa prosekom od 0.64. Vrednosti Ritlandovog koeficijenta srodnosti varirale su između 0.787 i -0.129. Osam markera je bilo dovoljno da razdvoji hibride sa jedinstvenim genotipovima. Analiza glavnih koordinata razdvojila je grupu ranih i grupu kasnih hibrida, iako je diferencijacija između grupa bila niska (Fst = 0.4%). Dva markera, bnlg1556 i umc1075, su najviše doprinela razdvajanju ranih i kasnih genotipova. Vrednosti parametara molekularnog genetičkog diverziteta bile su veće kod ranih nego kod kasnih hibirida, što ukazuje na širu genetičku osnovu prve grupe. Visok nivo polimorfizma markera ukazuje na njihovu podobnost za određivanje molekularnog profila hibirida (tzv. fingerprinting).