Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia
Molekularna karakterizacija i identifikacija gljiva uzročnika raka stabla uljane repice u Srbiji
Апстракт
During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as well as from the leaf, flower and pods. Two reference isolates from Great Britain were used in these studies for the analysis of 119 isolates from Serbia, using polymerase chain reaction (PCR) and PCR-RFLP. Digestion of PCR products was performed with 5 selected endonucleases: BamHI, HaeIII, Rsal, EcoRII, and AluI. On the basis of PCR analysis, all isolates originating from Serbia belong to L. complex: 111 belong to species Leptosphaeria maculans, and 8 to species Leptosphaeria biglobosa NA1 ('Leptosphaeria biglobosa brassicae), respectively.
Kulture gljiva izolovane su iz obolelih biljnih delova uljane repice koji su prikupljeni iz devet lokaliteta (Karavukovo, Crvenka, Prigrevica, Subotica, Rimski šančevi, Srbobran, Beška, Banatsko Karađorđevo, Srpski Miletić) u periodu od 2008 do 2010. godine. Uzorci obolelog tkiva su uzeti sa korena, prizemnog stabla, gornjeg dela stabla i grana, kao i sa lista, cveta, ljuski i semena. Molekularna karakterizacija je sprovedena korišćenjem lančane reakcije polimeraze PCR i PCR-RFLP, a ukupno je ispitivano 119 izolata poreklom iz Srbije i dva referentna izolata iz Velike Britanije. Digestija PCR produkata je sprovedena sa 5 odabranih endonukleaza: BamHI, HaeIII, RsaI, EcoRII i AluI. Na osnovu PCR analize, svi izolati poreklom iz Srbije pripadaju L. kompleksu: 111 pripada vrsti Leptosphaeria maculans, a 8 vrsti Leptosphaeria biglobosa NA1 (Leptosphaeria biglobosa brassicae).
Кључне речи:
Brassica napus / endonucleases / isolates / Leptosphaeria maculans / Leptosphaeria biglobosa / localities / PCR and PCR-RFLP / Brassica napus / endonukleaze / izolati / Leptosphaeria maculans / Leptosphaeria biglobosa / lokalitet / PCR and PCR -RFLPИзвор:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2017, 54, 2, 56-60Издавач:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Финансирање / пројекти:
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
Колекције
Институција/група
FiVeRTY - JOUR AU - Mitrović, Petar AU - Marjanović-Jeromela, Ana AU - Milovac, Željko AU - Radić, Velimir PY - 2017 UR - http://fiver.ifvcns.rs/handle/123456789/1640 AB - During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as well as from the leaf, flower and pods. Two reference isolates from Great Britain were used in these studies for the analysis of 119 isolates from Serbia, using polymerase chain reaction (PCR) and PCR-RFLP. Digestion of PCR products was performed with 5 selected endonucleases: BamHI, HaeIII, Rsal, EcoRII, and AluI. On the basis of PCR analysis, all isolates originating from Serbia belong to L. complex: 111 belong to species Leptosphaeria maculans, and 8 to species Leptosphaeria biglobosa NA1 ('Leptosphaeria biglobosa brassicae), respectively. AB - Kulture gljiva izolovane su iz obolelih biljnih delova uljane repice koji su prikupljeni iz devet lokaliteta (Karavukovo, Crvenka, Prigrevica, Subotica, Rimski šančevi, Srbobran, Beška, Banatsko Karađorđevo, Srpski Miletić) u periodu od 2008 do 2010. godine. Uzorci obolelog tkiva su uzeti sa korena, prizemnog stabla, gornjeg dela stabla i grana, kao i sa lista, cveta, ljuski i semena. Molekularna karakterizacija je sprovedena korišćenjem lančane reakcije polimeraze PCR i PCR-RFLP, a ukupno je ispitivano 119 izolata poreklom iz Srbije i dva referentna izolata iz Velike Britanije. Digestija PCR produkata je sprovedena sa 5 odabranih endonukleaza: BamHI, HaeIII, RsaI, EcoRII i AluI. Na osnovu PCR analize, svi izolati poreklom iz Srbije pripadaju L. kompleksu: 111 pripada vrsti Leptosphaeria maculans, a 8 vrsti Leptosphaeria biglobosa NA1 (Leptosphaeria biglobosa brassicae). PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research T1 - Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia T1 - Molekularna karakterizacija i identifikacija gljiva uzročnika raka stabla uljane repice u Srbiji EP - 60 IS - 2 SP - 56 VL - 54 DO - 10.5937/ratpov54-12683 ER -
@article{ author = "Mitrović, Petar and Marjanović-Jeromela, Ana and Milovac, Željko and Radić, Velimir", year = "2017", abstract = "During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as well as from the leaf, flower and pods. Two reference isolates from Great Britain were used in these studies for the analysis of 119 isolates from Serbia, using polymerase chain reaction (PCR) and PCR-RFLP. Digestion of PCR products was performed with 5 selected endonucleases: BamHI, HaeIII, Rsal, EcoRII, and AluI. On the basis of PCR analysis, all isolates originating from Serbia belong to L. complex: 111 belong to species Leptosphaeria maculans, and 8 to species Leptosphaeria biglobosa NA1 ('Leptosphaeria biglobosa brassicae), respectively., Kulture gljiva izolovane su iz obolelih biljnih delova uljane repice koji su prikupljeni iz devet lokaliteta (Karavukovo, Crvenka, Prigrevica, Subotica, Rimski šančevi, Srbobran, Beška, Banatsko Karađorđevo, Srpski Miletić) u periodu od 2008 do 2010. godine. Uzorci obolelog tkiva su uzeti sa korena, prizemnog stabla, gornjeg dela stabla i grana, kao i sa lista, cveta, ljuski i semena. Molekularna karakterizacija je sprovedena korišćenjem lančane reakcije polimeraze PCR i PCR-RFLP, a ukupno je ispitivano 119 izolata poreklom iz Srbije i dva referentna izolata iz Velike Britanije. Digestija PCR produkata je sprovedena sa 5 odabranih endonukleaza: BamHI, HaeIII, RsaI, EcoRII i AluI. Na osnovu PCR analize, svi izolati poreklom iz Srbije pripadaju L. kompleksu: 111 pripada vrsti Leptosphaeria maculans, a 8 vrsti Leptosphaeria biglobosa NA1 (Leptosphaeria biglobosa brassicae).", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research", title = "Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia, Molekularna karakterizacija i identifikacija gljiva uzročnika raka stabla uljane repice u Srbiji", pages = "60-56", number = "2", volume = "54", doi = "10.5937/ratpov54-12683" }
Mitrović, P., Marjanović-Jeromela, A., Milovac, Ž.,& Radić, V.. (2017). Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research Institut za ratarstvo i povrtarstvo, Novi Sad., 54(2), 56-60. https://doi.org/10.5937/ratpov54-12683
Mitrović P, Marjanović-Jeromela A, Milovac Ž, Radić V. Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2017;54(2):56-60. doi:10.5937/ratpov54-12683 .
Mitrović, Petar, Marjanović-Jeromela, Ana, Milovac, Željko, Radić, Velimir, "Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 54, no. 2 (2017):56-60, https://doi.org/10.5937/ratpov54-12683 . .