The use of genetic markers for the identification and estimation of vegetable plant species

Abstract

Isozymes as genetic markers were used for identification and genetic characterization of vegetable species such as cabbage, cauliflower, cucumber, carrot, tomato, and pepper. The following groups of marker systems were used: MDH, PGM, PHI and DIA with stable phenotypic expression. Certain genotypes were in the stage of seedling or seed. Polymorphism of the above mentioned systems was found in all the species analyzed which made possible the genetic identification of plant species and variability within them. These methods belonging to biochemical genetics were used for quality seed control. The results showed that, on the basis of these markers as direct gene products, certain pepper genotypes, activity and polymorphism (PHI, PGM, DIA) were found which pointed out genetically pure seed or some problems connected with quality traits.