Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars
Apstrakt
A set of 42 winter wheat cultivars developed from nine populations were evaluated for distinctness. Three marker systems: morphological markers, gliadin allele profiles and microsatellites were used to analyze distinctness between sister cultivars. The morphological based distinctness tests for wheat are based on a crop-specific set of characters that comply with UPOV guidelines. The morphological markers were quite informative although they were not capable to estimate the distances/distinctness between two pair of sister cultivars Evropa 90 and Evropa and Novosadska rana 3 and Novosadska rana 2. The gliadins allele profiles were the least efficient to estimate distinctness between sister line cultivars. This system was not capable to distinguish even cultivars developed from different populations. Sister line wheat cultivars were fingerprinted with 19 wheat microsatellites markers. A total of 106 alleles were detected at 19 wheat microsatellite loci, resulting in an average allele nu...mber per marker of 5.6. The number of markers was sufficient to distinguish among most sister line cultivars. Only one pair of sister cultivars, Loznicanka and Kosovka, derived from the same cross could not be distinguished. Comparing all marker systems to evaluate distinctness of sister line wheat cultivars the most efficient was microsatellite markers while gliadin allele profiles was the least efficient. Correlations between matrices based on pedigree data and morphological marker, gliadin profiles and microsatellites were significant but not large.
Ključne reči:
Distinctness / Gliadin allele profiles / Morphological markers / Microsatellite markers / Sister line cultivar / WheatIzvor:
Genetika-Belgrade, 2015, 47, 1, 219-232Izdavač:
- Društvo genetičara Srbije, Beograd
Finansiranje / projekti:
- Savremeno oplemenjivanje strnih žita za sadašnje i buduće potrebe (RS-MESTD-Technological Development (TD or TR)-31066)
DOI: 10.2298/GENSR1501219D
ISSN: 0534-0012
WoS: 000355248900019
Scopus: 2-s2.0-84929335100
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Denčić, Srbislav AU - DePauw, Ron AU - Momčilović, Vojislava AU - Kondić-Špika, Ankica PY - 2015 UR - http://fiver.ifvcns.rs/handle/123456789/1437 AB - A set of 42 winter wheat cultivars developed from nine populations were evaluated for distinctness. Three marker systems: morphological markers, gliadin allele profiles and microsatellites were used to analyze distinctness between sister cultivars. The morphological based distinctness tests for wheat are based on a crop-specific set of characters that comply with UPOV guidelines. The morphological markers were quite informative although they were not capable to estimate the distances/distinctness between two pair of sister cultivars Evropa 90 and Evropa and Novosadska rana 3 and Novosadska rana 2. The gliadins allele profiles were the least efficient to estimate distinctness between sister line cultivars. This system was not capable to distinguish even cultivars developed from different populations. Sister line wheat cultivars were fingerprinted with 19 wheat microsatellites markers. A total of 106 alleles were detected at 19 wheat microsatellite loci, resulting in an average allele number per marker of 5.6. The number of markers was sufficient to distinguish among most sister line cultivars. Only one pair of sister cultivars, Loznicanka and Kosovka, derived from the same cross could not be distinguished. Comparing all marker systems to evaluate distinctness of sister line wheat cultivars the most efficient was microsatellite markers while gliadin allele profiles was the least efficient. Correlations between matrices based on pedigree data and morphological marker, gliadin profiles and microsatellites were significant but not large. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars EP - 232 IS - 1 SP - 219 VL - 47 DO - 10.2298/GENSR1501219D ER -
@article{ author = "Denčić, Srbislav and DePauw, Ron and Momčilović, Vojislava and Kondić-Špika, Ankica", year = "2015", abstract = "A set of 42 winter wheat cultivars developed from nine populations were evaluated for distinctness. Three marker systems: morphological markers, gliadin allele profiles and microsatellites were used to analyze distinctness between sister cultivars. The morphological based distinctness tests for wheat are based on a crop-specific set of characters that comply with UPOV guidelines. The morphological markers were quite informative although they were not capable to estimate the distances/distinctness between two pair of sister cultivars Evropa 90 and Evropa and Novosadska rana 3 and Novosadska rana 2. The gliadins allele profiles were the least efficient to estimate distinctness between sister line cultivars. This system was not capable to distinguish even cultivars developed from different populations. Sister line wheat cultivars were fingerprinted with 19 wheat microsatellites markers. A total of 106 alleles were detected at 19 wheat microsatellite loci, resulting in an average allele number per marker of 5.6. The number of markers was sufficient to distinguish among most sister line cultivars. Only one pair of sister cultivars, Loznicanka and Kosovka, derived from the same cross could not be distinguished. Comparing all marker systems to evaluate distinctness of sister line wheat cultivars the most efficient was microsatellite markers while gliadin allele profiles was the least efficient. Correlations between matrices based on pedigree data and morphological marker, gliadin profiles and microsatellites were significant but not large.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars", pages = "232-219", number = "1", volume = "47", doi = "10.2298/GENSR1501219D" }
Denčić, S., DePauw, R., Momčilović, V.,& Kondić-Špika, A.. (2015). Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 47(1), 219-232. https://doi.org/10.2298/GENSR1501219D
Denčić S, DePauw R, Momčilović V, Kondić-Špika A. Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars. in Genetika-Belgrade. 2015;47(1):219-232. doi:10.2298/GENSR1501219D .
Denčić, Srbislav, DePauw, Ron, Momčilović, Vojislava, Kondić-Špika, Ankica, "Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars" in Genetika-Belgrade, 47, no. 1 (2015):219-232, https://doi.org/10.2298/GENSR1501219D . .