Transferring of Sclerotinia resistance from wild into cultivated sunflower: Combining of conventional and laboratory techniques

Abstract

Five populations of each H. molis, H. maximiliani, H. rigidus and H. tuberosus were screened for resistance to stem form of Sclerotinia. On the basis of the results obtained by screening, nine crosses of resistant populations with either other wild species populations or with cultivated sunflower were made. As in some crosses a small quantity of seed was produced and the seeds germinated poorly, modified tissue culture methods were used to enhance germination and produce clones of interesting plants. These methods were found to be efficient both for seed germination and plant production and multiplication.

Testirano je po pet populacija H. molis, H. maximiliani, H. rigidus i H. tuberosus na otpornost prema Sclerotinia stabla. Na osnovu dobijenih rezultata izvršeno je devet ukrštanja otpornih populacija sa drugim divljim vrstama ili gajenim suncokretom. Kako je u nekim slučajevima dobijena mala količina semena koja su slabo klijala, korišćene su modifikovane metode kulture tkiva da bi se povećala klijavost i proizveli klonovi interesantnih biljaka. Ove metode su se pokazale efikasnima i za naklijavanje semena i za proizvodnju i umnožavanje biljaka.