Detection of genetically modified soybean in crude soybean oil
Authorized Users Only
2014
Authors
Nikolić, ZoricaVasiljević, Ivana
Petrović, Gordana
Đorđević, Vuk
Ignjatov, Maja
Jovičić, Dušica
Milošević, Dragana
Article (Published version)
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In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil.
Keywords:
GMO / Soybean / Crude oilSource:
Food Chemistry, 2014, 145, 1072-1075Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- Increasing the market significance of forage crops by breeding and optimizing seed production technology (RS-MESTD-Technological Development (TD or TR)-31024)
DOI: 10.1016/j.foodchem.2013.09.017
ISSN: 0308-8146
PubMed: 24128586
WoS: 000327685200147
Scopus: 2-s2.0-84884668506
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FiVeRTY - JOUR AU - Nikolić, Zorica AU - Vasiljević, Ivana AU - Petrović, Gordana AU - Đorđević, Vuk AU - Ignjatov, Maja AU - Jovičić, Dušica AU - Milošević, Dragana PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1337 AB - In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil. PB - Elsevier Sci Ltd, Oxford T2 - Food Chemistry T1 - Detection of genetically modified soybean in crude soybean oil EP - 1075 SP - 1072 VL - 145 DO - 10.1016/j.foodchem.2013.09.017 ER -
@article{ author = "Nikolić, Zorica and Vasiljević, Ivana and Petrović, Gordana and Đorđević, Vuk and Ignjatov, Maja and Jovičić, Dušica and Milošević, Dragana", year = "2014", abstract = "In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Food Chemistry", title = "Detection of genetically modified soybean in crude soybean oil", pages = "1075-1072", volume = "145", doi = "10.1016/j.foodchem.2013.09.017" }
Nikolić, Z., Vasiljević, I., Petrović, G., Đorđević, V., Ignjatov, M., Jovičić, D.,& Milošević, D.. (2014). Detection of genetically modified soybean in crude soybean oil. in Food Chemistry Elsevier Sci Ltd, Oxford., 145, 1072-1075. https://doi.org/10.1016/j.foodchem.2013.09.017
Nikolić Z, Vasiljević I, Petrović G, Đorđević V, Ignjatov M, Jovičić D, Milošević D. Detection of genetically modified soybean in crude soybean oil. in Food Chemistry. 2014;145:1072-1075. doi:10.1016/j.foodchem.2013.09.017 .
Nikolić, Zorica, Vasiljević, Ivana, Petrović, Gordana, Đorđević, Vuk, Ignjatov, Maja, Jovičić, Dušica, Milošević, Dragana, "Detection of genetically modified soybean in crude soybean oil" in Food Chemistry, 145 (2014):1072-1075, https://doi.org/10.1016/j.foodchem.2013.09.017 . .