Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean
2014
Autori
Petrović, GordanaNikolić, Zorica
Đorđević, Vuk
Župunski, Vesna
Jovičić, Dušica
Ignjatov, Maja
Milošević, Dragana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The objective of this work was to evaluate the genetic variation of trypsin inhibitor in cultivated (Glycine max L.) and wild (Glycine sofa Siebold & Zucc.) soybean varieties. Genetic variations of the Kunitz trypsin inhibitor, represented by a 21-kD protein (KTI), and of the Bowman-Birk trypsin chymotrypsin inhibitor (BBI) were evaluated in cultivated (G. max) and wild (G. sofa) soybean varieties. Endonuclease heteroduplex mismatch cleavage assays were performed to detect mutations in the KTI gene, with a single-stranded specific nuclease obtained from celery extracts (CEL I). The investigated soybean varieties showed low level of genetic variation in KTI and BBI. PCR-RFLP analysis divided the BBI-A type into subtypes A1 and A2, and showed that Tib type of KTI is the dominant type. Digestion with restriction enzymes was not able to detect differences between ti-null and other types of Ti alleles, while the endonuclease heteroduplex mismatch cleavage assay with CEL I could detect ti-nu...ll type. The digestion method with CEL I provides a simple and useful genetic tool for SNP analysis. The presented method can be used as a tool for fast and useful screening of desired genotypes in future breeding programs of soybean.
Ključne reči:
Glycine max / Glycine soja / antinutritional factors / protease inhibitors / SNPIzvor:
Pesquisa Agropecuaria Brasileira, 2014, 49, 2, 102-108Izdavač:
- Empresa Brasil Pesq Agropec, Brasilia Df
Finansiranje / projekti:
- Povećanje tržišnog značaja krmnih biljaka oplemenjivanjem i optimizacijom tehnologije proizvodnje semena (RS-MESTD-Technological Development (TD or TR)-31024)
- Interdisciplinarni pristup stvaranju novih sorti soje i unapređenju tehnologije gajenja i dorade semena (RS-MESTD-Technological Development (TD or TR)-31022)
DOI: 10.1590/S0100-204X2014000200004
ISSN: 0100-204X
WoS: 000337718300004
Scopus: 2-s2.0-84903450881
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Petrović, Gordana AU - Nikolić, Zorica AU - Đorđević, Vuk AU - Župunski, Vesna AU - Jovičić, Dušica AU - Ignjatov, Maja AU - Milošević, Dragana PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1302 AB - The objective of this work was to evaluate the genetic variation of trypsin inhibitor in cultivated (Glycine max L.) and wild (Glycine sofa Siebold & Zucc.) soybean varieties. Genetic variations of the Kunitz trypsin inhibitor, represented by a 21-kD protein (KTI), and of the Bowman-Birk trypsin chymotrypsin inhibitor (BBI) were evaluated in cultivated (G. max) and wild (G. sofa) soybean varieties. Endonuclease heteroduplex mismatch cleavage assays were performed to detect mutations in the KTI gene, with a single-stranded specific nuclease obtained from celery extracts (CEL I). The investigated soybean varieties showed low level of genetic variation in KTI and BBI. PCR-RFLP analysis divided the BBI-A type into subtypes A1 and A2, and showed that Tib type of KTI is the dominant type. Digestion with restriction enzymes was not able to detect differences between ti-null and other types of Ti alleles, while the endonuclease heteroduplex mismatch cleavage assay with CEL I could detect ti-null type. The digestion method with CEL I provides a simple and useful genetic tool for SNP analysis. The presented method can be used as a tool for fast and useful screening of desired genotypes in future breeding programs of soybean. PB - Empresa Brasil Pesq Agropec, Brasilia Df T2 - Pesquisa Agropecuaria Brasileira T1 - Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean EP - 108 IS - 2 SP - 102 VL - 49 DO - 10.1590/S0100-204X2014000200004 ER -
@article{ author = "Petrović, Gordana and Nikolić, Zorica and Đorđević, Vuk and Župunski, Vesna and Jovičić, Dušica and Ignjatov, Maja and Milošević, Dragana", year = "2014", abstract = "The objective of this work was to evaluate the genetic variation of trypsin inhibitor in cultivated (Glycine max L.) and wild (Glycine sofa Siebold & Zucc.) soybean varieties. Genetic variations of the Kunitz trypsin inhibitor, represented by a 21-kD protein (KTI), and of the Bowman-Birk trypsin chymotrypsin inhibitor (BBI) were evaluated in cultivated (G. max) and wild (G. sofa) soybean varieties. Endonuclease heteroduplex mismatch cleavage assays were performed to detect mutations in the KTI gene, with a single-stranded specific nuclease obtained from celery extracts (CEL I). The investigated soybean varieties showed low level of genetic variation in KTI and BBI. PCR-RFLP analysis divided the BBI-A type into subtypes A1 and A2, and showed that Tib type of KTI is the dominant type. Digestion with restriction enzymes was not able to detect differences between ti-null and other types of Ti alleles, while the endonuclease heteroduplex mismatch cleavage assay with CEL I could detect ti-null type. The digestion method with CEL I provides a simple and useful genetic tool for SNP analysis. The presented method can be used as a tool for fast and useful screening of desired genotypes in future breeding programs of soybean.", publisher = "Empresa Brasil Pesq Agropec, Brasilia Df", journal = "Pesquisa Agropecuaria Brasileira", title = "Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean", pages = "108-102", number = "2", volume = "49", doi = "10.1590/S0100-204X2014000200004" }
Petrović, G., Nikolić, Z., Đorđević, V., Župunski, V., Jovičić, D., Ignjatov, M.,& Milošević, D.. (2014). Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean. in Pesquisa Agropecuaria Brasileira Empresa Brasil Pesq Agropec, Brasilia Df., 49(2), 102-108. https://doi.org/10.1590/S0100-204X2014000200004
Petrović G, Nikolić Z, Đorđević V, Župunski V, Jovičić D, Ignjatov M, Milošević D. Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean. in Pesquisa Agropecuaria Brasileira. 2014;49(2):102-108. doi:10.1590/S0100-204X2014000200004 .
Petrović, Gordana, Nikolić, Zorica, Đorđević, Vuk, Župunski, Vesna, Jovičić, Dušica, Ignjatov, Maja, Milošević, Dragana, "Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean" in Pesquisa Agropecuaria Brasileira, 49, no. 2 (2014):102-108, https://doi.org/10.1590/S0100-204X2014000200004 . .