Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits
2013
Authors
Živančev, DraganNikolovski, Branislava G.
Torbica, Aleksandra
Mastilović, Jasna
Đukić, Nevena
Article (Published version)
Metadata
Show full item recordAbstract
Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molec...ular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step.
Keywords:
Arija wheat variety / Glutenin subunits / Lab-on-a-Chip / LoaC Lab-on-a-Chip / WheatSource:
Chemical Industry & Chemical Engineering Quarterly, 2013, 19, 4, 553-561Publisher:
- Savez hemijskih inženjera, Beograd
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.2298/CICEQ120517090Z
ISSN: 1451-9372
WoS: 000328965400009
Scopus: 2-s2.0-84890701949
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FiVeRTY - JOUR AU - Živančev, Dragan AU - Nikolovski, Branislava G. AU - Torbica, Aleksandra AU - Mastilović, Jasna AU - Đukić, Nevena PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/1292 AB - Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molecular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step. PB - Savez hemijskih inženjera, Beograd T2 - Chemical Industry & Chemical Engineering Quarterly T1 - Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits EP - 561 IS - 4 SP - 553 VL - 19 DO - 10.2298/CICEQ120517090Z ER -
@article{ author = "Živančev, Dragan and Nikolovski, Branislava G. and Torbica, Aleksandra and Mastilović, Jasna and Đukić, Nevena", year = "2013", abstract = "Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molecular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Chemical Industry & Chemical Engineering Quarterly", title = "Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits", pages = "561-553", number = "4", volume = "19", doi = "10.2298/CICEQ120517090Z" }
Živančev, D., Nikolovski, B. G., Torbica, A., Mastilović, J.,& Đukić, N.. (2013). Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits. in Chemical Industry & Chemical Engineering Quarterly Savez hemijskih inženjera, Beograd., 19(4), 553-561. https://doi.org/10.2298/CICEQ120517090Z
Živančev D, Nikolovski BG, Torbica A, Mastilović J, Đukić N. Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits. in Chemical Industry & Chemical Engineering Quarterly. 2013;19(4):553-561. doi:10.2298/CICEQ120517090Z .
Živančev, Dragan, Nikolovski, Branislava G., Torbica, Aleksandra, Mastilović, Jasna, Đukić, Nevena, "Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits" in Chemical Industry & Chemical Engineering Quarterly, 19, no. 4 (2013):553-561, https://doi.org/10.2298/CICEQ120517090Z . .