Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)
Abstract
Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of... the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.
Keywords:
Helianthus maximiliani / repetitive somatic embryogenesis / secondary somatic embryogenesisSource:
Plant Cell Reports, 2001, 20, 2, 121-125Publisher:
- Springer, New York
DOI: 10.1007/s002990000291
ISSN: 0721-7714
PubMed: 30759897
WoS: 000167189500003
Scopus: 2-s2.0-0035114412
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FiVeRTY - JOUR AU - Vasić, Dragana AU - Alibert, Gilbert AU - Škorić, Dragan PY - 2001 UR - http://fiver.ifvcns.rs/handle/123456789/96 AB - Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos. PB - Springer, New York T2 - Plant Cell Reports T1 - Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader) EP - 125 IS - 2 SP - 121 VL - 20 DO - 10.1007/s002990000291 ER -
@article{ author = "Vasić, Dragana and Alibert, Gilbert and Škorić, Dragan", year = "2001", abstract = "Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.", publisher = "Springer, New York", journal = "Plant Cell Reports", title = "Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)", pages = "125-121", number = "2", volume = "20", doi = "10.1007/s002990000291" }
Vasić, D., Alibert, G.,& Škorić, D.. (2001). Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader). in Plant Cell Reports Springer, New York., 20(2), 121-125. https://doi.org/10.1007/s002990000291
Vasić D, Alibert G, Škorić D. Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader). in Plant Cell Reports. 2001;20(2):121-125. doi:10.1007/s002990000291 .
Vasić, Dragana, Alibert, Gilbert, Škorić, Dragan, "Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)" in Plant Cell Reports, 20, no. 2 (2001):121-125, https://doi.org/10.1007/s002990000291 . .