Sunflower inbred lines screening for tolerance to white rot on stalk

Abstract

Seventy sunflower (Helianthus annuus L) inbred lines were screened for tolerance to white rot on stalk. Plants were inoculated at the budding stage with 4-day old Sclerotinia mycelium grown on PDA medium. Mycelium was placed on the leaf top and covered with tin foil, and the leaf was put into transparent nylon bag in order to maintain high humidity. Spot length on leaf was measured and plant tolerance was determined at the full flowering stage. Obtained results were analyzed by analysis of variance. Tested lines showed significant variability in response to disease. Eleven lines had lesion length less than 50% compared to susceptible control, so they could be considered tolerant and potentially interesting for breeding program.

Sedamdeset inbred linija suncokreta (Helianthus annuus L) je testirano na tolerantnost prema prouzrokovaču bele truleži stabla. Biljke su inokulisane u fazi butonizacije sa 4 dana starom kolonijom gljive gajenom na PDA podlozi. Delovi podloge sa micelijom su stavljeni na vrh lista, prekriveni aluminijumskom folijom i potom providnom najlonskom kesom da bi se obezbedio visok nivo vlažnosti. Merena je dužina pega i nivo tolerantnosti u fazi punog cvetanja suncokreta. Testirane linije su pokazale značajnu varijabilnost u reakciji prema patogenu, a 11 linija čija je prosečna relativna dužina pega iznosila ispod 50% u odnosu na osetljivu kontrolu su označene kao tolerantne i potencijalno interesantne za uključivanje u proces oplemenjivanja.