Application of genetic markers in seed testing and plant breeding

Abstract

Genetic markers have been used at Institute of Field and Vegetable Crops in Novi Sad for a number of years, both for seed quality control and for research purposes. The Laboratory for Seed Testing was the first in the former Yugoslavia to use the method of control of hybrid seed genetic purity based on enzymatic polymorphism. This paper presents the application of protein markers, isozymes, seed storage proteins and DNA markers for evaluation of seed and breeding materials of various agricultural crops in Serbia.

Genetički markeri se dugi niz godina koriste u Institutu za ratarstvo i povrtarstvo u Novom Sadu u kontroli kvaliteta semena i u istraživačke svrhe. Laboratorija za ispitivanje semena je prva u bivšoj Jugoslaviji uvela metod kontrole genetičke čistoće semena zasnovan na polimorfizmu enzima. U radu je dat pregled primene proteinskih markera, izoenzima i rezervnih proteina semena, kao i DNK markera u oceni kvaliteta semena i materijala za oplemenjivanje različitih biljnih vrsta u Srbiji.