Comparative analysis of laboratory and field procedures for testing genetic purity of sunflower parent lines

Abstract

One of the main features that confer high quality to the seed is its genetic purity. Genetic impurities in sunflower seed lot can originate from seed due to self-pollination, uncontrolled cross-pollination or mechanical mixing of seeds. Genetic purity of 18 seed lots of sunflower parental lines is controlled by comparative analysis of morphological traits and electrophoretic analysis 4 isoenzyme systems, according to the UPOV guidelines for DUS test of sunflower lines and hybrids. In 83.3% of the total analysed samples, results in the field and laboratory were in agreement. In parental line R-8 5% genetic impurity was found by isoenzyme analysis, which was not detected based on morphological characteristics, while electrophoretic analysis of samples L-10 and L-11 showed less genetic impurities in comparison with the analysis in the field.

Jedna od važnih komponenti kvaliteta semena jeste njegova sortna, odnosno genetska čistoća. Uzroci nesortnih primesa mogu poticati od semena nastalog usled nekontrolisane samooplodnje, stranooplodnje ili usled mehaničkog mešanja semena. Genetska čistoća 18 partija roditeljskih linija suncokreta kontrolisana je uporednom analizom morfoloških osobina i elektroforetskom analizom 4 izoenzimska sistema, prema UPOV uputstvu za DUS analizu linija i hibrida suncokreta. U 83,3% od ukupno ispitanih uzoraka, rezultati u polju i laboratoriji su bili u saglasnosti. Kod roditeljske linije R-8 analizom izoenzima pronađeno je 5% genetske nečistoće koja nije konstatovana na osnovu morfoloških karakteristika, dok je elektroforetska analiza uzoraka L-10 i L-11 pokazala manju genetsku nečistoću u poređenju sa analizom u polju.