Reliability of NIR method in protein and oil content determination in soybean

Abstract

A primary characteristic of diffuse reflectance spectroscopy is fast and non-destructive determination of constituent concentration in products. The main goal is to find the nature of absorption (depending of wave length) and compare it with stable standard. NIR spectroscopy is a technology based on real number of molecules of each constituent and represents highly applicable analytical method. After calibration development or calibration transfer from one to another NIR instrument, it is necessary to verify the slope and bias of calibration. Results from multi year experiments show bias changes, while slope was constant, implicating that calibration changes had translator nature.

Primarna karakteristika difuzne refleksne spektroskopije je brzo i nedestruktivno određivanje sadržaja stvarnih konstituenata u proizvodima. Cilj je da se tačno zabeleži priroda apsorpcije (koja je zavisna od talasne dužine) u odnosu na stabilni standard. NIR spektroskopija je tehnologija koja je uglavnom zasnovana na stvarnom broju molekula svakog pojedinačnog konstituenta i teoretski predstavlja visoko-primenljivu analitičku metodu. Nakon konstrukcije nove kalibracione krive, prilikom transfera kalibracije sa jednog na drugi NIR instrument, potrebno je proveriti nagib i pomeranje kalibracione krive. Dobijeni rezultati višegodišnjih ispitivanja pokazuju pomeranje kalibracione krive, dok je nagib krive ostao nepromenjen, što znači da je navedeno pomeranje translatorne prirode.