Occurrence and distribution of viruses infecting the bean in Serbia

Abstract

This work describes the incidence and distribution of the most important bean viruses in Serbia: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The viral isolates were characterized serologically and biologically. BCMV was found in the largest number of plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), and AMV (3.41%), since BYMV was not determined. Mixed viral infections were found in several samples. The RT-PCR method was used to prove that the tested isolates belong to the BCMV, family Potyviridae and strains Russian and NL-3 D. Results obtained in this work will enable further studies of the genetic variability of bean virus isolates from Serbia.

Cilj istraživanja je bio utvrđivanje prisustva i rasprostranjenosti ekonomski najštetnijih virusa pasulja na području Vojvodine: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Alfalfa mosaic virus (AMV) i Cucumber mosaic virus (CMV). Izolati virusa okarakterisani su serološki i biološki. U najvećem broju uzoraka konstatovan je BCMV (30,53%), u znatno manjem broju konstatovani su BCMNV (2,67%), CMV (5,35%), i AMV (3,41%), dok nijedan uzorak nije pokazao pozitivnu reakciju na BYMV. U nekoliko uzoraka je utvrđena mešana infekcije virusa. RT-PCR metoda je korišćena da bi se utvrdilo da li ispitivani izolati pripadaju BCMV, fam. Potyviridae i sojevima Russian i NL-3D. Rezultati dobijeni u ovom radu će omogućiti dalja istraživanja genetičke varijabilnosti izolata virusa pasulja iz Srbija.