Subunit composition of seed storage proteins in high-protein soybean genotypes
Апстракт
The objective of this work was to quantify the accumulation of the major seed storage protein subunits, beta-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and beta-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all subunits of glycinin and beta-conglycinin, were the only exceptions. Attention should be given to genotypes KO5314 and KO5317, which accumulated significantly higher amounts of both subunits of glycinin, and to genotypes KO5425, KO5...319, KO539 and KO536, which accumulated significantly higher amounts of beta-conglycinin subunits. These findings suggest that some of the tested genotypes could be beneficial in different breeding programs aimed at the production of agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications.
Кључне речи:
Glycine max / beta-conglycinin / glycinin / protein content / subunit compositionИзвор:
Pesquisa Agropecuaria Brasileira, 2010, 45, 7, 721-729Издавач:
- Empresa Brasil Pesq Agropec, Brasilia Df
Финансирање / пројекти:
- Serbian Ministry of Science and Technological Development
DOI: 10.1590/S0100-204X2010000700013
ISSN: 0100-204X
WoS: 000282654000013
Scopus: 2-s2.0-77957237718
Колекције
Институција/група
FiVeRTY - JOUR AU - Taški-Ajduković, Ksenija AU - Đorđević, Vuk AU - Vidić, Miloš AU - Vujaković, Milka PY - 2010 UR - http://fiver.ifvcns.rs/handle/123456789/834 AB - The objective of this work was to quantify the accumulation of the major seed storage protein subunits, beta-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and beta-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all subunits of glycinin and beta-conglycinin, were the only exceptions. Attention should be given to genotypes KO5314 and KO5317, which accumulated significantly higher amounts of both subunits of glycinin, and to genotypes KO5425, KO5319, KO539 and KO536, which accumulated significantly higher amounts of beta-conglycinin subunits. These findings suggest that some of the tested genotypes could be beneficial in different breeding programs aimed at the production of agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications. PB - Empresa Brasil Pesq Agropec, Brasilia Df T2 - Pesquisa Agropecuaria Brasileira T1 - Subunit composition of seed storage proteins in high-protein soybean genotypes EP - 729 IS - 7 SP - 721 VL - 45 DO - 10.1590/S0100-204X2010000700013 ER -
@article{ author = "Taški-Ajduković, Ksenija and Đorđević, Vuk and Vidić, Miloš and Vujaković, Milka", year = "2010", abstract = "The objective of this work was to quantify the accumulation of the major seed storage protein subunits, beta-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and beta-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all subunits of glycinin and beta-conglycinin, were the only exceptions. Attention should be given to genotypes KO5314 and KO5317, which accumulated significantly higher amounts of both subunits of glycinin, and to genotypes KO5425, KO5319, KO539 and KO536, which accumulated significantly higher amounts of beta-conglycinin subunits. These findings suggest that some of the tested genotypes could be beneficial in different breeding programs aimed at the production of agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications.", publisher = "Empresa Brasil Pesq Agropec, Brasilia Df", journal = "Pesquisa Agropecuaria Brasileira", title = "Subunit composition of seed storage proteins in high-protein soybean genotypes", pages = "729-721", number = "7", volume = "45", doi = "10.1590/S0100-204X2010000700013" }
Taški-Ajduković, K., Đorđević, V., Vidić, M.,& Vujaković, M.. (2010). Subunit composition of seed storage proteins in high-protein soybean genotypes. in Pesquisa Agropecuaria Brasileira Empresa Brasil Pesq Agropec, Brasilia Df., 45(7), 721-729. https://doi.org/10.1590/S0100-204X2010000700013
Taški-Ajduković K, Đorđević V, Vidić M, Vujaković M. Subunit composition of seed storage proteins in high-protein soybean genotypes. in Pesquisa Agropecuaria Brasileira. 2010;45(7):721-729. doi:10.1590/S0100-204X2010000700013 .
Taški-Ajduković, Ksenija, Đorđević, Vuk, Vidić, Miloš, Vujaković, Milka, "Subunit composition of seed storage proteins in high-protein soybean genotypes" in Pesquisa Agropecuaria Brasileira, 45, no. 7 (2010):721-729, https://doi.org/10.1590/S0100-204X2010000700013 . .