Correlation of antioxidant activity with polyphenolic content in soybeans
Апстракт
Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flav...onoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics.
Кључне речи:
soybeans / isoflavones / polyphenolic content / antioxidant activity / DPPH assayИзвор:
Oxidation Communications, 2009, 32, 3, 697-706Колекције
Институција/група
FiVeRTY - JOUR AU - Tepavčević, Vesna AU - Cvejić, Jelena AU - Malenčić, Ðorđe AU - Miladinović, Jegor AU - Popović, Jovan PY - 2009 UR - http://fiver.ifvcns.rs/handle/123456789/766 AB - Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flavonoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics. T2 - Oxidation Communications T1 - Correlation of antioxidant activity with polyphenolic content in soybeans EP - 706 IS - 3 SP - 697 VL - 32 UR - https://hdl.handle.net/21.15107/rcub_fiver_766 ER -
@article{ author = "Tepavčević, Vesna and Cvejić, Jelena and Malenčić, Ðorđe and Miladinović, Jegor and Popović, Jovan", year = "2009", abstract = "Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flavonoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics.", journal = "Oxidation Communications", title = "Correlation of antioxidant activity with polyphenolic content in soybeans", pages = "706-697", number = "3", volume = "32", url = "https://hdl.handle.net/21.15107/rcub_fiver_766" }
Tepavčević, V., Cvejić, J., Malenčić, Ð., Miladinović, J.,& Popović, J.. (2009). Correlation of antioxidant activity with polyphenolic content in soybeans. in Oxidation Communications, 32(3), 697-706. https://hdl.handle.net/21.15107/rcub_fiver_766
Tepavčević V, Cvejić J, Malenčić Ð, Miladinović J, Popović J. Correlation of antioxidant activity with polyphenolic content in soybeans. in Oxidation Communications. 2009;32(3):697-706. https://hdl.handle.net/21.15107/rcub_fiver_766 .
Tepavčević, Vesna, Cvejić, Jelena, Malenčić, Ðorđe, Miladinović, Jegor, Popović, Jovan, "Correlation of antioxidant activity with polyphenolic content in soybeans" in Oxidation Communications, 32, no. 3 (2009):697-706, https://hdl.handle.net/21.15107/rcub_fiver_766 .