Cytological characteristics of regenerants obtained from anther culture of wheat (triticum aestivum l.)

Abstract

The aim, of this study was to investigate ploidy levels of regenerated green plants obtained from anther culture of wheat (Triticum aestivum L). Anthers were isolated from 20 F, hybrid combinations and grown on Potato-2 inductive medium. Calluses were transferred to the 190-2 regeneration medium on which 145 green plants were obtained. The cytological analysis showed that, out of the total number of green plants, 42.8% were haploids (2n = 3x = 21) and 53.8% were spontaneous double haploids (2n = 6x = 42). Only 3.4% regenerated green plants were aneuploids (2n not equal 21, 42).