Shoot development from hypocotyl protoplasts of sunflower (helianthus annuus l.)
Само за регистроване кориснике
2009
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated microcalluses to solid regeneration media. Shoot regeneration was achieved by culture of calluses on SE regeneration medium after the treatment with 2.2 mu M thidiazuron.
Кључне речи:
Helianthus / protoplasts / regeneration / sunflowerИзвор:
Acta Biologica Hungarica, 2009, 60, 2, 233-239Издавач:
- Akademiai Kiado Zrt, Budapest
Финансирање / пројекти:
- Стварање високо продуктивних генотипова сунцокрета (Helianthus annuus L.) (RS-MESTD-MPN2006-2010-20080)
DOI: 10.1556/ABiol.60.2009.2.10
ISSN: 0236-5383
PubMed: 19584032
WoS: 000268416500010
Scopus: 2-s2.0-69249130893
Колекције
Институција/група
FiVeRTY - JOUR AU - Taški-Ajduković, Ksenija AU - Nagl, Nevena AU - Miladinović, Dragana AU - Mikić, Aleksandar PY - 2009 UR - http://fiver.ifvcns.rs/handle/123456789/710 AB - The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated microcalluses to solid regeneration media. Shoot regeneration was achieved by culture of calluses on SE regeneration medium after the treatment with 2.2 mu M thidiazuron. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Biologica Hungarica T1 - Shoot development from hypocotyl protoplasts of sunflower (helianthus annuus l.) EP - 239 IS - 2 SP - 233 VL - 60 DO - 10.1556/ABiol.60.2009.2.10 ER -
@article{ author = "Taški-Ajduković, Ksenija and Nagl, Nevena and Miladinović, Dragana and Mikić, Aleksandar", year = "2009", abstract = "The plant regeneration from sunflower protoplast was achieved by protocols that considerably differ with respect to basal medium and concentration of growth regulators added. In most cases regeneration was restricted to a small proportion of genotypes. In this experiment, sunflower inbred line Ha-74A, with a high regeneration ability was used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in agarose droplets and cultured according to different regeneration protocols. The best results were obtained when protoplasts were cultured on L4 medium followed by transfer of regenerated microcalluses to solid regeneration media. Shoot regeneration was achieved by culture of calluses on SE regeneration medium after the treatment with 2.2 mu M thidiazuron.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Biologica Hungarica", title = "Shoot development from hypocotyl protoplasts of sunflower (helianthus annuus l.)", pages = "239-233", number = "2", volume = "60", doi = "10.1556/ABiol.60.2009.2.10" }
Taški-Ajduković, K., Nagl, N., Miladinović, D.,& Mikić, A.. (2009). Shoot development from hypocotyl protoplasts of sunflower (helianthus annuus l.). in Acta Biologica Hungarica Akademiai Kiado Zrt, Budapest., 60(2), 233-239. https://doi.org/10.1556/ABiol.60.2009.2.10
Taški-Ajduković K, Nagl N, Miladinović D, Mikić A. Shoot development from hypocotyl protoplasts of sunflower (helianthus annuus l.). in Acta Biologica Hungarica. 2009;60(2):233-239. doi:10.1556/ABiol.60.2009.2.10 .
Taški-Ajduković, Ksenija, Nagl, Nevena, Miladinović, Dragana, Mikić, Aleksandar, "Shoot development from hypocotyl protoplasts of sunflower (helianthus annuus l.)" in Acta Biologica Hungarica, 60, no. 2 (2009):233-239, https://doi.org/10.1556/ABiol.60.2009.2.10 . .