Genetic purity of sunflower hybrids determined on the basis of isozymes and seed storage proteins
Апстракт
Maintenance of genetic uniformity of lines and hybrids is a prerequisite for successful production and placement of commercial hybrid seed on the market. Genetic purity of seed as specific seed trait is of great significance for seed science. Protein markers, seed storage proteins and isozymes, which are commonly used for the estimation of genetic purity, were used in this work to estimate genetic purity in sunflower hybrids. Analysis of helianthinin revealed tree zymogram patterns within and between sixteen sunflower hybrids. All of the 6 enzymatic systems analyzed, MDH, PGM, PHI, PGD, IDH and ACP, were polymorphic. A comparative analysis of genetic purity level of the sunflower hybrids was performed using electrophoretic methods. The methods of electrophoretic separation of isozymes and seed storage proteins were in agreement, with differences ranging from 1% to 5% in 81% of the samples. The level of polymorphism obtained by both methods was not distinct enough to be used in genotype... identification.
Кључне речи:
Genetic purity / Isozyme / Seed storage proteinИзвор:
Helia, 2008, 31, 48, 47-54Издавач:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Колекције
Институција/група
FiVeRTY - JOUR AU - Nikolić, Zorica AU - Vujaković, Milka AU - Jevtić, Aranka PY - 2008 UR - http://fiver.ifvcns.rs/handle/123456789/653 AB - Maintenance of genetic uniformity of lines and hybrids is a prerequisite for successful production and placement of commercial hybrid seed on the market. Genetic purity of seed as specific seed trait is of great significance for seed science. Protein markers, seed storage proteins and isozymes, which are commonly used for the estimation of genetic purity, were used in this work to estimate genetic purity in sunflower hybrids. Analysis of helianthinin revealed tree zymogram patterns within and between sixteen sunflower hybrids. All of the 6 enzymatic systems analyzed, MDH, PGM, PHI, PGD, IDH and ACP, were polymorphic. A comparative analysis of genetic purity level of the sunflower hybrids was performed using electrophoretic methods. The methods of electrophoretic separation of isozymes and seed storage proteins were in agreement, with differences ranging from 1% to 5% in 81% of the samples. The level of polymorphism obtained by both methods was not distinct enough to be used in genotype identification. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Helia T1 - Genetic purity of sunflower hybrids determined on the basis of isozymes and seed storage proteins EP - 54 IS - 48 SP - 47 VL - 31 DO - 10.2298/HEL0848047N ER -
@article{ author = "Nikolić, Zorica and Vujaković, Milka and Jevtić, Aranka", year = "2008", abstract = "Maintenance of genetic uniformity of lines and hybrids is a prerequisite for successful production and placement of commercial hybrid seed on the market. Genetic purity of seed as specific seed trait is of great significance for seed science. Protein markers, seed storage proteins and isozymes, which are commonly used for the estimation of genetic purity, were used in this work to estimate genetic purity in sunflower hybrids. Analysis of helianthinin revealed tree zymogram patterns within and between sixteen sunflower hybrids. All of the 6 enzymatic systems analyzed, MDH, PGM, PHI, PGD, IDH and ACP, were polymorphic. A comparative analysis of genetic purity level of the sunflower hybrids was performed using electrophoretic methods. The methods of electrophoretic separation of isozymes and seed storage proteins were in agreement, with differences ranging from 1% to 5% in 81% of the samples. The level of polymorphism obtained by both methods was not distinct enough to be used in genotype identification.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Helia", title = "Genetic purity of sunflower hybrids determined on the basis of isozymes and seed storage proteins", pages = "54-47", number = "48", volume = "31", doi = "10.2298/HEL0848047N" }
Nikolić, Z., Vujaković, M.,& Jevtić, A.. (2008). Genetic purity of sunflower hybrids determined on the basis of isozymes and seed storage proteins. in Helia Institut za ratarstvo i povrtarstvo, Novi Sad., 31(48), 47-54. https://doi.org/10.2298/HEL0848047N
Nikolić Z, Vujaković M, Jevtić A. Genetic purity of sunflower hybrids determined on the basis of isozymes and seed storage proteins. in Helia. 2008;31(48):47-54. doi:10.2298/HEL0848047N .
Nikolić, Zorica, Vujaković, Milka, Jevtić, Aranka, "Genetic purity of sunflower hybrids determined on the basis of isozymes and seed storage proteins" in Helia, 31, no. 48 (2008):47-54, https://doi.org/10.2298/HEL0848047N . .