The effect of naphthenic acids on white poplar (Populus alba L.) microcuttings' rooting

Abstract

Naphtenic acids, by-product in oil refinement, are well known soil pollutants and active substances in preparations for plant and wood protection. The results of multiannual research indicated the possibility of their utilization for stimulation of cutting rooting. Two to three cm high shoots of Populus alba cl. LBM genotype were set for one hour on ACM medium with 4, 16 or 48 μM concentration of indol-3-butyric acid, 1-naphthalene acetic acid or Na-salts of naphthenic acids (for which the effect of 10 and 30 minutes treatment on medium with 48 μM was also examined). Then, treated micro-shoots were transferred on ACM medium without hormones. The differences among treatments were the most distinguishable for rooting percentage after two weeks of cultivation. One-hour treatment on the medium with 16 μM and 30 minutes treatment on medium with 48 μM of Na-naphthenates gave the best results. .

Naftenske kiseline, nusproizvodi u procesu rafinacije nafte, su poznati zagađivači zemljišta i aktivna materija sredstava za zaštitu biljka i drveta. Rezultati višegodišnjih istraživanja ukazuju i na mogućnost njihove upotrebe kao sredstava za stimulaciju oživljavanja reznica. Dva do tri cm visoki mikroizbojci genotipa Populus alba cl. LBM su jedan čas bili postavljeni na ACM podlozi sa 4, 16 ili 48 μM koncentracijom indolbuterne kiseline, naftilsirćetne kiseline ili Na-soli naftenskih kiselina (kod kojih je ispitan i uticaj 10-to i 30-to minutnog tretmana pri koncentraciji 48 μM). Zatim su mikroizbojci postavljeni na ACM podlogu bez hormona. Razlike među tretmanima su se najviše ispoljile u procentu ožiljavanja nakon dve nedelje kultivacije. Jednočasovni tretmani na podlozi sa 16 μM i polučasovni na podlozi sa 48 μM Na-naftenata su dali najbolje rezultate. .