Unveiling Fungi Armor: Preliminary Study on Fortifying Pisum sativum L. Seeds against Drought with Schizophyllum commune Fries 1815 Polysaccharide Fractions
Аутори
Mišković, JovanaTamindžić, Gordana
![](/themes/MirageFiVeR/images/orcid.png)
Rašeta, Milena
Ignjatov, Maja
![](/themes/MirageFiVeR/images/orcid.png)
Krsmanović, Nenad
Gojgić-Cvijović, Gordana
Karaman, Maja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Amidst worsening climate change, drought stress imperils global agriculture, jeopardizing crop yields and food security, thereby necessitating the urgent exploration of sustainable methods like biopriming for the harnessing of beneficial microorganisms to bolster plant resilience. Recent research has revealed diverse biological compounds with versatile applications produced by Schizophyllum commune, rendering this fungus as a promising contender for biopriming applications. For the first time, this study aimed to investigate the potential of S. commune exo- (EPSH) and intra-polysaccharides (IPSH) isolated from two strains—Italian (ITA) and Serbian (SRB)—under submerged cultivation to enhance the resilience of Pisum sativum L. seeds through the biopriming technique. Testing of the seed quality for the bioprimed, hydroprimed, and unprimed seeds was conducted using a germination test, under optimal and drought conditions, while characterization of the PSHs included FTIR analysis, microana...lysis, and determination of total protein content (TPC). The FTIR spectra of EPSH and IPSH were very similar but revealed the impurities, while microanalysis and TPC confirmed a different presence of proteins in the isolated PSHs. In optimal conditions, the IPSH SRB increased germination energy by 5.50% compared to the control; however, the highest percentage of germination (94.70%) was shown after biopriming with the PSH isolated from the ITA strain. Additionally, all assessed treatments resulted in a boost in seedling growth and biomass accumulation, where the ITA strain demonstrated greater effectiveness in optimal conditions, while the SRB strain showed superiority in drought conditions. The drought tolerance indices increased significantly in response to all examined treatments during the drought, with EPSH ITA (23.00%) and EPSH SRB (24.00%) demonstrating the greatest effects. Results of this preliminary study demonstrate the positive effect of isolated PSH, indicating their potential as biopriming agents and offering insights into novel strategies for agricultural resilience.
Кључне речи:
agricultural application / fungi / biopriming / stress conditions / submerged cultivation / Schizophyllum commune / Pisum sativumИзвор:
Microorganisms, 2024, 12, 6, 1107-Издавач:
- Basel : MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200125 (Универзитет у Новом Саду, Природно-математички факултет) (RS-MESTD-inst-2020-200125)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
Колекције
Институција/група
FiVeRTY - JOUR AU - Mišković, Jovana AU - Tamindžić, Gordana AU - Rašeta, Milena AU - Ignjatov, Maja AU - Krsmanović, Nenad AU - Gojgić-Cvijović, Gordana AU - Karaman, Maja PY - 2024 UR - http://fiver.ifvcns.rs/handle/123456789/4730 AB - Amidst worsening climate change, drought stress imperils global agriculture, jeopardizing crop yields and food security, thereby necessitating the urgent exploration of sustainable methods like biopriming for the harnessing of beneficial microorganisms to bolster plant resilience. Recent research has revealed diverse biological compounds with versatile applications produced by Schizophyllum commune, rendering this fungus as a promising contender for biopriming applications. For the first time, this study aimed to investigate the potential of S. commune exo- (EPSH) and intra-polysaccharides (IPSH) isolated from two strains—Italian (ITA) and Serbian (SRB)—under submerged cultivation to enhance the resilience of Pisum sativum L. seeds through the biopriming technique. Testing of the seed quality for the bioprimed, hydroprimed, and unprimed seeds was conducted using a germination test, under optimal and drought conditions, while characterization of the PSHs included FTIR analysis, microanalysis, and determination of total protein content (TPC). The FTIR spectra of EPSH and IPSH were very similar but revealed the impurities, while microanalysis and TPC confirmed a different presence of proteins in the isolated PSHs. In optimal conditions, the IPSH SRB increased germination energy by 5.50% compared to the control; however, the highest percentage of germination (94.70%) was shown after biopriming with the PSH isolated from the ITA strain. Additionally, all assessed treatments resulted in a boost in seedling growth and biomass accumulation, where the ITA strain demonstrated greater effectiveness in optimal conditions, while the SRB strain showed superiority in drought conditions. The drought tolerance indices increased significantly in response to all examined treatments during the drought, with EPSH ITA (23.00%) and EPSH SRB (24.00%) demonstrating the greatest effects. Results of this preliminary study demonstrate the positive effect of isolated PSH, indicating their potential as biopriming agents and offering insights into novel strategies for agricultural resilience. PB - Basel : MDPI T2 - Microorganisms T1 - Unveiling Fungi Armor: Preliminary Study on Fortifying Pisum sativum L. Seeds against Drought with Schizophyllum commune Fries 1815 Polysaccharide Fractions IS - 6 SP - 1107 VL - 12 DO - 10.3390/microorganisms12061107 ER -
@article{ author = "Mišković, Jovana and Tamindžić, Gordana and Rašeta, Milena and Ignjatov, Maja and Krsmanović, Nenad and Gojgić-Cvijović, Gordana and Karaman, Maja", year = "2024", abstract = "Amidst worsening climate change, drought stress imperils global agriculture, jeopardizing crop yields and food security, thereby necessitating the urgent exploration of sustainable methods like biopriming for the harnessing of beneficial microorganisms to bolster plant resilience. Recent research has revealed diverse biological compounds with versatile applications produced by Schizophyllum commune, rendering this fungus as a promising contender for biopriming applications. For the first time, this study aimed to investigate the potential of S. commune exo- (EPSH) and intra-polysaccharides (IPSH) isolated from two strains—Italian (ITA) and Serbian (SRB)—under submerged cultivation to enhance the resilience of Pisum sativum L. seeds through the biopriming technique. Testing of the seed quality for the bioprimed, hydroprimed, and unprimed seeds was conducted using a germination test, under optimal and drought conditions, while characterization of the PSHs included FTIR analysis, microanalysis, and determination of total protein content (TPC). The FTIR spectra of EPSH and IPSH were very similar but revealed the impurities, while microanalysis and TPC confirmed a different presence of proteins in the isolated PSHs. In optimal conditions, the IPSH SRB increased germination energy by 5.50% compared to the control; however, the highest percentage of germination (94.70%) was shown after biopriming with the PSH isolated from the ITA strain. Additionally, all assessed treatments resulted in a boost in seedling growth and biomass accumulation, where the ITA strain demonstrated greater effectiveness in optimal conditions, while the SRB strain showed superiority in drought conditions. The drought tolerance indices increased significantly in response to all examined treatments during the drought, with EPSH ITA (23.00%) and EPSH SRB (24.00%) demonstrating the greatest effects. Results of this preliminary study demonstrate the positive effect of isolated PSH, indicating their potential as biopriming agents and offering insights into novel strategies for agricultural resilience.", publisher = "Basel : MDPI", journal = "Microorganisms", title = "Unveiling Fungi Armor: Preliminary Study on Fortifying Pisum sativum L. Seeds against Drought with Schizophyllum commune Fries 1815 Polysaccharide Fractions", number = "6", pages = "1107", volume = "12", doi = "10.3390/microorganisms12061107" }
Mišković, J., Tamindžić, G., Rašeta, M., Ignjatov, M., Krsmanović, N., Gojgić-Cvijović, G.,& Karaman, M.. (2024). Unveiling Fungi Armor: Preliminary Study on Fortifying Pisum sativum L. Seeds against Drought with Schizophyllum commune Fries 1815 Polysaccharide Fractions. in Microorganisms Basel : MDPI., 12(6), 1107. https://doi.org/10.3390/microorganisms12061107
Mišković J, Tamindžić G, Rašeta M, Ignjatov M, Krsmanović N, Gojgić-Cvijović G, Karaman M. Unveiling Fungi Armor: Preliminary Study on Fortifying Pisum sativum L. Seeds against Drought with Schizophyllum commune Fries 1815 Polysaccharide Fractions. in Microorganisms. 2024;12(6):1107. doi:10.3390/microorganisms12061107 .
Mišković, Jovana, Tamindžić, Gordana, Rašeta, Milena, Ignjatov, Maja, Krsmanović, Nenad, Gojgić-Cvijović, Gordana, Karaman, Maja, "Unveiling Fungi Armor: Preliminary Study on Fortifying Pisum sativum L. Seeds against Drought with Schizophyllum commune Fries 1815 Polysaccharide Fractions" in Microorganisms, 12, no. 6 (2024):1107, https://doi.org/10.3390/microorganisms12061107 . .