Detection of allelic variability at wheat loci associated with resistance to Fusarium ssp. using molecular markers - microsatellites

Abstract

Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is one of the most important fungal diseases of wheat. It may cause severe yield and quality losses in humid and warm conditions. However, the most important concern is the mycotoxin contamination of grain. Breeding of cultivars resistant to FHB is the best way to control the disease. In order to examine possibilities for application molecular markers - microsatellites in selecting for resistance to FHB, allelic variability at wheat loci associated with resistance to Fusarium ssp. was saidied using two microsatellite markers: GWM533 (chromosome 3B) and GWM156 (chromosome 5A). Detection of the allelic polymorphism was conducted compared to the cultivars Sumai 3, Frontana and Amigo which are widely used as FHB resistance sources. In 23 French genotypes and 25 genotypes developed at the Institute of Field and Vegetable Crops in Novi Sad, 5 alleles (GWM533) and 6 alleles (GWM156) were detected. In 15 genotypes at loci GWM533 and 10 genotypes at loci GWM156 same allele was detected as in some of standard cultivars. Obtained results should be evaluated at field experiments in order to confirm corelation between presence of specific allele and resistance to fusarium head blight and in order to examine importance of alleles that are not detected in standard cultivars.

Analizirana je alelna varijabilnost lokusa heksaploidne pšenice značajnih za otpornost na fuzarijum primenom dva mikrosatelitska markera: GWM533 (hromozom 3B) i GWM156 (bromozom 5A), sa ciljem iznalaženja mogućnostiprimene molekularnih markera - mikrosatelita pri selekciji materijala za navedeno svojstvo. Pri detekciji alelnogpolimorfizma, kao standardi korišćene su najpoznatije sorte izvori otpornosti: Sumai3, Frontana i Amigo. Kod 23 francuska i 25 novosadskih genotipova pšenice utvrđeno je prisustvo 5 alela (GWM533) i 6 alela (GWM156). Utvrđene su iPIC (Polymorphism information content) vrednosti za ispitivane markere, koje su iznosile 0,783 (GWM533) i 0,747 (GWM156). Ulokusu GWM533 kod 15 genotipova ustanovljeno je prisustvo istog alela koji je registrovan i kod neke od standardnih sorti, a u lokusu GWM156 kod 10 ispitivanih genotipova.