Mogućnost određivanja rezidua acetamiprida i njegovog metabolita u trešnjama primenom HPLC/DAD

Abstract

U radu je razvijena brza i jednostavna metoda za istovremeno određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline (6HNK) u uzorcima trešanja. Metoda se bazira na primeni reverzne faze razdvajanja na C18 koloni primenom gradijentne elucije. Za određivanje i kvantifikaciju analita primenjena je tečna hromatografija sa detektorom sa nizom diada (HPLC/DAD), pri talasnoj dužini od 230 nm. Ekstrakcija acetamiprida 6HNK izvedena je mešavinom acetonitril/0.IN NHyCl. Prosečne vrednosti prinosa ekstrakcije aceiamiprida 6HNK u uzorcima trešanja kretale su se u intervalima od 95-101% 1 73-83% (RSD<5%), respektivno,

A rapid and simple method for the simultaneous analysis and quantification of acetamiprid and 6-chloronicotinie acid (6CNA). as intermediate of acetamiprid decomposition in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C8 column with gradient elution. Analytes’ determination and quantification were performed by HPLC/DAD and chromatograms were extracted at 230 nm. These insecticides were extracted with mixture acetonitril/0.1N NH)CL Average recoveries of acekumiprid and 6CNA from sweet cherry samples were in range between 95-101 % and 73- $3%, respectively. with RSDs<5%.