Androgenous ability of heterozygous wheat genotypes and cytological characteristics of green regenerants

Abstract

Androgenous and regeneration abilities of 8 heterozygous wheat (Triticum aestivum L.) genotypes were analyzed in anther culture. Cytological characteristics of green regenerants were also analyzed. Significant differences were found among the genotypes in their androgenous and regeneration abilities. The average androgenous capacity of all genotypes was 4.4% ranging from 0.7% (Prima/Hueguen) to 11.0% (NS173-98/NS164-98). The average callus yield was 8.7%. The genotype Banks/F53-70 produced the highest number of green plants (33 green plants), while the genotype Banks/Pobeda showed no green plant regeneration ability. The frequency of albino plants ranged between 0% (Banks/Rodna) and 5.3% (Anastasija/Radika). A total of 61 green and 61 albino plants were regenerated. The results of cytological analysis showed that, from the total number of regenerated green plants, 40.9% were haploids and 59.1% were spontaneous double haploids.

Analizirana je androgena i regeneraciona sposobnost kod 8 heterozigotnih genotipova pšenice (Triticum aestivum L.) u kulturi antera. Urađena je i citološka analiza regenerisanih zelenih biljaka. Utvrđene su statistički značajne razlike između ispitivanih genotipova u pogledu androgene i regeneracione sposobnosti. Prosečna androgena sposobnost za sve ispitivane genotipove iznosila je 4,4%, a kretala se od 0,7% kod genotipa Prima/Hueguen do 11,0% kod genotipa NS173-98/NS164-98. Prosečan prinos kalusa bio je 8,7%. Najviše zelenih biljaka dao je genotip Banks/F53-70 (33 zelene biljke) dok genotip Banks/Pobeda nije regenerisao ni jednu zelenu biljku. Frekvencija albino biljaka kretala se između 0% (Banks/Rodna) i 5,3% (Anastasija/Radika). Ukupno je regenerisano 61 zelena i 61 albino biljka. Rezultati citološke analize pokazali su da su, od ukupnog broja regenerisanih zelenih biljaka, 40,9% bili haploidi, a 59,1% spontani dvostruki haploidi.