FiVeR - Repository of the Institute of Field and Vegetable Crops
Institute of Field and Vegetable Crops
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus

Citogenetska ispitivanja heksaploidne vrste Helianthus tuberosus i njenih F1 i BC1F1 hibrida sa gajenim suncokretom, H. annuus

Thumbnail
2006
354.pdf (236.1Kb)
Authors
Atlagić, Jovanka
Terzić, Sreten
Article (Published version)
Metadata
Show full item record
Abstract
Helianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F] and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using aceto-carmine method (GEORGIEVA-TODOROVA, 1976) and pollen viability was determined by staining method of ALEXANDER (1969). Meiosis was regular in cultivated sunflower and the pollen viability was high (96.8-98.9%). Low percent of irregularities was found in the meiosis of H. tuberosus. Pollen viability was high (97.2-98.7%). Chromosome pairing was mostly regular in F1 hybrids (34 bivalents), but some meiocytes contained 28-32 bivalents with uni- and quadrivalents present. The percent of meiocytes with fast chromosomes in metaphase was 24.6-87.2, with lagging chromosomes in anaphase I 10.5-81.0 and in telophase 25.0-33.3. Chromosome bridges were detected in 0-9.9% of meiocytes in anaphase. Po...llen viability in F1 hybrids ranged from 27.0 to 47.9%. In BC1F1 hybrids number of bivalents was 16-25, univalent 2-18 and multivalent 0-1. Although a triploid set of chromosomes (51) was expected in BC1F1 hybrids, number of chromosomes was 45-57. Pollen viability varied from 0 to 54.3%.

H. tuberosus predstavlja potencijalni izvor otpornosti prema mnogim prouzrokovačima bolesti. U istraživanjima su korišćene tri populacije vrste H. tuberosus koje su ukrštane sa gajenim suncokretom. Dobijeno je 6 hibridnih kombinacija F1 i 2 BC1F1 gen. Izvršena je analiza mejoze acetokarmin metodom (Georgieva-Todorova, 1976) i vitalnosti polena bojenom metodom (Alexander, 1969). Mejoza kod gajenog suncokreta proticala je normalno, a vitalnost polena bila visoka (96.8-98.9%). Kod vrste H. tuberosus mejoza je proticala uz nizak % nepravilnosti tipa neuključenih hromozoma. Vitalnost polena je bila visoka (97.2-98.7%). Kod F1 hibrida najčešće je bilo normalno parenje hromozoma (34 bivalenta), a zabeležene su mejocite sa 28-32 bivalenta uz pojavu uni- i kvadrivalenata. Procenat mejocita sa izbeglim hromozomima u metafazi I je bio 24.6-87.2, sa izostalim hromozomima u anafazi I 10.5-81.0, a u telofazi II 25.0-33.3. Hromozomski mostovi su detektovani u 0-9.9% anafaznih mejocita. Vitalnost pole...na kod FI hibrida se kretala od 27.0-47.9%. Kod BC1F1 hibrida broj bivalenaca je bio od 16-25, univalenata od 2-18, a multivalenata od 0-1. Iako je očekivan triploidan broj hromozoma (51) kod BC1F1 hibrida broj hromozoma je bio od 45-57. Vitalnost polena se kretala 0-54.3%.

Keywords:
cultivated sunflower / Helianthus tuberosus / interspecific hybridization / meiosis / pollen viability
Source:
Genetika-Belgrade, 2006, 38, 3, 203-213
Publisher:
  • Društvo genetičara Srbije, Beograd

DOI: 10.2298/GENSR0603203A

ISSN: 0534-0012

[ Google Scholar ]
URI
http://fiver.ifvcns.rs/handle/123456789/357
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Atlagić, Jovanka
AU  - Terzić, Sreten
PY  - 2006
UR  - http://fiver.ifvcns.rs/handle/123456789/357
AB  - Helianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F] and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using aceto-carmine method (GEORGIEVA-TODOROVA, 1976) and pollen viability was determined by staining method of ALEXANDER (1969). Meiosis was regular in cultivated sunflower and the pollen viability was high (96.8-98.9%). Low percent of irregularities was found in the meiosis of H. tuberosus. Pollen viability was high (97.2-98.7%). Chromosome pairing was mostly regular in F1 hybrids (34 bivalents), but some meiocytes contained 28-32 bivalents with uni- and quadrivalents present. The percent of meiocytes with fast chromosomes in metaphase was 24.6-87.2, with lagging chromosomes in anaphase I 10.5-81.0 and in telophase 25.0-33.3. Chromosome bridges were detected in 0-9.9% of meiocytes in anaphase. Pollen viability in F1 hybrids ranged from 27.0 to 47.9%. In BC1F1 hybrids number of bivalents was 16-25, univalent 2-18 and multivalent 0-1. Although a triploid set of chromosomes (51) was expected in BC1F1 hybrids, number of chromosomes was 45-57. Pollen viability varied from 0 to 54.3%.
AB  - H. tuberosus predstavlja potencijalni izvor otpornosti prema mnogim prouzrokovačima bolesti. U istraživanjima su korišćene tri populacije vrste H. tuberosus koje su ukrštane sa gajenim suncokretom. Dobijeno je 6 hibridnih kombinacija F1 i 2 BC1F1 gen. Izvršena je analiza mejoze acetokarmin metodom (Georgieva-Todorova, 1976) i vitalnosti polena bojenom metodom (Alexander, 1969). Mejoza kod gajenog suncokreta proticala je normalno, a vitalnost polena bila visoka (96.8-98.9%). Kod vrste H. tuberosus mejoza je proticala uz nizak % nepravilnosti tipa neuključenih hromozoma. Vitalnost polena je bila visoka (97.2-98.7%). Kod F1 hibrida najčešće je bilo normalno parenje hromozoma (34 bivalenta), a zabeležene su mejocite sa 28-32 bivalenta uz pojavu uni- i kvadrivalenata. Procenat mejocita sa izbeglim hromozomima u metafazi I je bio 24.6-87.2, sa izostalim hromozomima u anafazi I 10.5-81.0, a u telofazi II 25.0-33.3. Hromozomski mostovi su detektovani u 0-9.9% anafaznih mejocita. Vitalnost polena kod FI hibrida se kretala od 27.0-47.9%. Kod BC1F1 hibrida broj bivalenaca je bio od 16-25, univalenata od 2-18, a multivalenata od 0-1. Iako je očekivan triploidan broj hromozoma (51) kod BC1F1 hibrida broj hromozoma je bio od 45-57. Vitalnost polena se kretala 0-54.3%.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus
T1  - Citogenetska ispitivanja heksaploidne vrste Helianthus tuberosus i njenih F1 i BC1F1 hibrida sa gajenim suncokretom, H. annuus
EP  - 213
IS  - 3
SP  - 203
VL  - 38
DO  - 10.2298/GENSR0603203A
UR  - conv_1350
ER  - 
@article{
author = "Atlagić, Jovanka and Terzić, Sreten",
year = "2006",
abstract = "Helianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F] and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using aceto-carmine method (GEORGIEVA-TODOROVA, 1976) and pollen viability was determined by staining method of ALEXANDER (1969). Meiosis was regular in cultivated sunflower and the pollen viability was high (96.8-98.9%). Low percent of irregularities was found in the meiosis of H. tuberosus. Pollen viability was high (97.2-98.7%). Chromosome pairing was mostly regular in F1 hybrids (34 bivalents), but some meiocytes contained 28-32 bivalents with uni- and quadrivalents present. The percent of meiocytes with fast chromosomes in metaphase was 24.6-87.2, with lagging chromosomes in anaphase I 10.5-81.0 and in telophase 25.0-33.3. Chromosome bridges were detected in 0-9.9% of meiocytes in anaphase. Pollen viability in F1 hybrids ranged from 27.0 to 47.9%. In BC1F1 hybrids number of bivalents was 16-25, univalent 2-18 and multivalent 0-1. Although a triploid set of chromosomes (51) was expected in BC1F1 hybrids, number of chromosomes was 45-57. Pollen viability varied from 0 to 54.3%., H. tuberosus predstavlja potencijalni izvor otpornosti prema mnogim prouzrokovačima bolesti. U istraživanjima su korišćene tri populacije vrste H. tuberosus koje su ukrštane sa gajenim suncokretom. Dobijeno je 6 hibridnih kombinacija F1 i 2 BC1F1 gen. Izvršena je analiza mejoze acetokarmin metodom (Georgieva-Todorova, 1976) i vitalnosti polena bojenom metodom (Alexander, 1969). Mejoza kod gajenog suncokreta proticala je normalno, a vitalnost polena bila visoka (96.8-98.9%). Kod vrste H. tuberosus mejoza je proticala uz nizak % nepravilnosti tipa neuključenih hromozoma. Vitalnost polena je bila visoka (97.2-98.7%). Kod F1 hibrida najčešće je bilo normalno parenje hromozoma (34 bivalenta), a zabeležene su mejocite sa 28-32 bivalenta uz pojavu uni- i kvadrivalenata. Procenat mejocita sa izbeglim hromozomima u metafazi I je bio 24.6-87.2, sa izostalim hromozomima u anafazi I 10.5-81.0, a u telofazi II 25.0-33.3. Hromozomski mostovi su detektovani u 0-9.9% anafaznih mejocita. Vitalnost polena kod FI hibrida se kretala od 27.0-47.9%. Kod BC1F1 hibrida broj bivalenaca je bio od 16-25, univalenata od 2-18, a multivalenata od 0-1. Iako je očekivan triploidan broj hromozoma (51) kod BC1F1 hibrida broj hromozoma je bio od 45-57. Vitalnost polena se kretala 0-54.3%.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus, Citogenetska ispitivanja heksaploidne vrste Helianthus tuberosus i njenih F1 i BC1F1 hibrida sa gajenim suncokretom, H. annuus",
pages = "213-203",
number = "3",
volume = "38",
doi = "10.2298/GENSR0603203A",
url = "conv_1350"
}
Atlagić, J.,& Terzić, S.. (2006). Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 38(3), 203-213.
https://doi.org/10.2298/GENSR0603203A
conv_1350
Atlagić J, Terzić S. Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus. in Genetika-Belgrade. 2006;38(3):203-213.
doi:10.2298/GENSR0603203A
conv_1350 .
Atlagić, Jovanka, Terzić, Sreten, "Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus" in Genetika-Belgrade, 38, no. 3 (2006):203-213,
https://doi.org/10.2298/GENSR0603203A .,
conv_1350 .

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB