During culture of protoplasts in agarose droplets, permanent problem was bacterial infection. It was assumed that the seeds are the origin of infection, so different sterilization methods were tested in order to overcome this problem. Germination, infection of seeds and hypocotyls and their growth were examined. Based on these parameters, the best result was obtained with the combined use of 5% commercial bleach and dry heating at 45°C.
Prilikom kultivacije protoplasta gajenog suncokreta u kapljicama agaroze stalan problem je bila bakterijska infekcija. Kako je pretpostavljeno da je seme izvor ove infekcije, isprobane su različite metode njegove sterilizacije da bi se pokušao prevazići ovaj problem. Praćeni su klijavost semena, broj infekcija semena i hipokotila, kao i njihov rast. Na osnovu ovih parametara najbolji rezultat je dobijen nakon kombinovane upotrebe 5% varikine i suve sterilizacije semena na 45°C.
TY - JOUR
AU - Taški-Ajduković, Ksenija
AU - Vasić, Dragana
PY - 2005
UR - http://fiver.ifvcns.rs/handle/123456789/322
AB - During culture of protoplasts in agarose droplets, permanent problem was bacterial infection. It was assumed that the seeds are the origin of infection, so different sterilization methods were tested in order to overcome this problem. Germination, infection of seeds and hypocotyls and their growth were examined. Based on these parameters, the best result was obtained with the combined use of 5% commercial bleach and dry heating at 45°C.
AB - Prilikom kultivacije protoplasta gajenog suncokreta u kapljicama agaroze stalan problem je bila bakterijska infekcija. Kako je pretpostavljeno da je seme izvor ove infekcije, isprobane su različite metode njegove sterilizacije da bi se pokušao prevazići ovaj problem. Praćeni su klijavost semena, broj infekcija semena i hipokotila, kao i njihov rast. Na osnovu ovih parametara najbolji rezultat je dobijen nakon kombinovane upotrebe 5% varikine i suve sterilizacije semena na 45°C.
PB - Matica srpska, Novi Sad
T2 - Zbornik Matice srpske za prirodne nauke
T1 - Different sterilization methods for overcoming internal bacterial infection in sunflower seeds
T1 - Upotreba različitih metoda sterilizacije semena suncokreta u prevazilaženju endogene bakterijske infekcije
EP - 64
IS - 109
SP - 59
DO - 10.2298/ZMSPN0519059T
UR - conv_493
ER -
@article{
author = "Taški-Ajduković, Ksenija and Vasić, Dragana",
year = "2005",
abstract = "During culture of protoplasts in agarose droplets, permanent problem was bacterial infection. It was assumed that the seeds are the origin of infection, so different sterilization methods were tested in order to overcome this problem. Germination, infection of seeds and hypocotyls and their growth were examined. Based on these parameters, the best result was obtained with the combined use of 5% commercial bleach and dry heating at 45°C., Prilikom kultivacije protoplasta gajenog suncokreta u kapljicama agaroze stalan problem je bila bakterijska infekcija. Kako je pretpostavljeno da je seme izvor ove infekcije, isprobane su različite metode njegove sterilizacije da bi se pokušao prevazići ovaj problem. Praćeni su klijavost semena, broj infekcija semena i hipokotila, kao i njihov rast. Na osnovu ovih parametara najbolji rezultat je dobijen nakon kombinovane upotrebe 5% varikine i suve sterilizacije semena na 45°C.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Different sterilization methods for overcoming internal bacterial infection in sunflower seeds, Upotreba različitih metoda sterilizacije semena suncokreta u prevazilaženju endogene bakterijske infekcije",
pages = "64-59",
number = "109",
doi = "10.2298/ZMSPN0519059T",
url = "conv_493"
}
Taški-Ajduković, K.,& Vasić, D.. (2005). Different sterilization methods for overcoming internal bacterial infection in sunflower seeds. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(109), 59-64.
https://doi.org/10.2298/ZMSPN0519059T
conv_493
Taški-Ajduković K, Vasić D. Different sterilization methods for overcoming internal bacterial infection in sunflower seeds. in Zbornik Matice srpske za prirodne nauke. 2005;(109):59-64.
doi:10.2298/ZMSPN0519059T
conv_493 .
Taški-Ajduković, Ksenija, Vasić, Dragana, "Different sterilization methods for overcoming internal bacterial infection in sunflower seeds" in Zbornik Matice srpske za prirodne nauke, no. 109 (2005):59-64,
https://doi.org/10.2298/ZMSPN0519059T .,
conv_493 .
