Analysis of storage proteins in a worldwide collection of pea seeds
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Peas (Pisum sativum L.) are an important source of vegetable protein for both humans and animals. However, pea seed proteins are limited in sulfur-containing amino acids (SCAA) and thus considered to be an insufficient source. The main seed proteins in pea are 7S (vicilins, convicilins) and 11S (legumins) globulins. Because 11S globulins contain more SCAA than 7S globulins, variations in the 7S/11S ratio affects the nutritional quality of pea seeds. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was used to separate seed proteins from 260 pea genotypes from around the world (the EUCLEG collection). Based on the electropherogram, 18 protein bands were densitometrically measured. Protein compositional data are intrinsically multivariate. The protein concentration of each genotype is presented in closed form i.e., sum up to 100%. Therefore, for multivariate data analysis specific statistical methodology was used. All methods used in the study were carried out in cla...ssical and robust manner that was resistant to outlier observations. The storage protein composition of the genotypes studied varied greatly; in some cases, only traces of the main components were found. The results suggest that some of the genotypes tested could be useful in various breeding programs aimed at producing agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications.
Кључне речи:
peas / pea collection / seed proteins / storage proteinsИзвор:
Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade, 2022, 106-106Издавач:
- Belgrade : Serbian Plant Physiology Society
- Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“
- Belgrade : University of Belgrade, Faculty of Biology
Финансирање / пројекти:
- EUCLEG - Breeding forage and grain legumes to increase EU's and China's protein self-sufficiency (EU-H2020-727312)
Колекције
Институција/група
FiVeRTY - CONF AU - Taški-Ajduković, Ksenija AU - Zorić, Miroslav AU - Živanov, Dalibor PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/3106 AB - Peas (Pisum sativum L.) are an important source of vegetable protein for both humans and animals. However, pea seed proteins are limited in sulfur-containing amino acids (SCAA) and thus considered to be an insufficient source. The main seed proteins in pea are 7S (vicilins, convicilins) and 11S (legumins) globulins. Because 11S globulins contain more SCAA than 7S globulins, variations in the 7S/11S ratio affects the nutritional quality of pea seeds. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was used to separate seed proteins from 260 pea genotypes from around the world (the EUCLEG collection). Based on the electropherogram, 18 protein bands were densitometrically measured. Protein compositional data are intrinsically multivariate. The protein concentration of each genotype is presented in closed form i.e., sum up to 100%. Therefore, for multivariate data analysis specific statistical methodology was used. All methods used in the study were carried out in classical and robust manner that was resistant to outlier observations. The storage protein composition of the genotypes studied varied greatly; in some cases, only traces of the main components were found. The results suggest that some of the genotypes tested could be useful in various breeding programs aimed at producing agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications. PB - Belgrade : Serbian Plant Physiology Society PB - Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“ PB - Belgrade : University of Belgrade, Faculty of Biology C3 - Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade T1 - Analysis of storage proteins in a worldwide collection of pea seeds EP - 106 SP - 106 UR - https://hdl.handle.net/21.15107/rcub_fiver_3106 ER -
@conference{ author = "Taški-Ajduković, Ksenija and Zorić, Miroslav and Živanov, Dalibor", year = "2022", abstract = "Peas (Pisum sativum L.) are an important source of vegetable protein for both humans and animals. However, pea seed proteins are limited in sulfur-containing amino acids (SCAA) and thus considered to be an insufficient source. The main seed proteins in pea are 7S (vicilins, convicilins) and 11S (legumins) globulins. Because 11S globulins contain more SCAA than 7S globulins, variations in the 7S/11S ratio affects the nutritional quality of pea seeds. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was used to separate seed proteins from 260 pea genotypes from around the world (the EUCLEG collection). Based on the electropherogram, 18 protein bands were densitometrically measured. Protein compositional data are intrinsically multivariate. The protein concentration of each genotype is presented in closed form i.e., sum up to 100%. Therefore, for multivariate data analysis specific statistical methodology was used. All methods used in the study were carried out in classical and robust manner that was resistant to outlier observations. The storage protein composition of the genotypes studied varied greatly; in some cases, only traces of the main components were found. The results suggest that some of the genotypes tested could be useful in various breeding programs aimed at producing agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications.", publisher = "Belgrade : Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“, Belgrade : University of Belgrade, Faculty of Biology", journal = "Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade", title = "Analysis of storage proteins in a worldwide collection of pea seeds", pages = "106-106", url = "https://hdl.handle.net/21.15107/rcub_fiver_3106" }
Taški-Ajduković, K., Zorić, M.,& Živanov, D.. (2022). Analysis of storage proteins in a worldwide collection of pea seeds. in Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade Belgrade : Serbian Plant Physiology Society., 106-106. https://hdl.handle.net/21.15107/rcub_fiver_3106
Taški-Ajduković K, Zorić M, Živanov D. Analysis of storage proteins in a worldwide collection of pea seeds. in Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade. 2022;:106-106. https://hdl.handle.net/21.15107/rcub_fiver_3106 .
Taški-Ajduković, Ksenija, Zorić, Miroslav, Živanov, Dalibor, "Analysis of storage proteins in a worldwide collection of pea seeds" in Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade (2022):106-106, https://hdl.handle.net/21.15107/rcub_fiver_3106 .