Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode

Abstract

Izlaganje stresnim uslovima životne sredine, kao što je suša, ima negativan efekat na rast biljaka. Abcisinska kiselina (ABA) se akumulira u uslovima osmotskog stresa i igra važnu ulogu u odgovoru na stres i u toleranciji biljaka. Cilj ovog istraživanja je bio da se odredi koncentracija ABA i ekspresija aldehid oksidaznog enzima (AO) kod 7 sorti stočnog graška u početnim fazama klijanja semena i u stadijumu trećeg para listova. Osmotski stres je stimulisan primenom PEG 6000. Stres suše je izazvan prekidanjem zalivanja. Koncentracija ABA je merena Elisa metodom. U nadzemnom delu ponika utvrđeno je značajno povećanje sadržaja ABA kod jedne grupe sorti (Javor, Partner, Junior) i smanjenje koncentracije ABA kod drugih sorti (Mraz, Dukat, Pionir, Trezor).

Exposure to to environmental stresses, such as drought, causes adverse effects on the growth of plants. Abscisic acid (ABA) accumulates under osmotic stress conditions and plays an important role in the stress response and tolerance of plants. The aim of this research was to determine the rates of ABA and the expression of Aldehyde oxidase enzyme (AO) among 7 cultivars of field pea at early seedling stage and at stage of third pair of leaves. Osmotic stress was stimulated using PEG 6000. The drought stress was induced by a termination of irrigation. ABA concentration was measured by the Elisa method. In the shoot of seedlings a significant increase in ABA content in one group of cultivars (Javor, Partner, Junior), and a decrease in ABA concentration in other cultivars (Mraz, Dukat, Pionir, Trezor) was confirmed.