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Evaluation of nonstarch polysaccharides and oligosaccharide content of different soybean varieties (Glycine max) by near-infrared spectroscopy and proteomics
dc.creator | Hollung, Kristin | |
dc.creator | Overland, Margareth | |
dc.creator | Hrustić, Milica | |
dc.creator | Sekulić, Petar | |
dc.creator | Miladinović, Jegor | |
dc.creator | Martens, Harald | |
dc.creator | Narum, Bjorg | |
dc.creator | Sahlstrom, Stefan | |
dc.creator | Sorensen, Mette | |
dc.creator | Storebakken, Trond | |
dc.creator | Skrede, Anders | |
dc.date.accessioned | 2021-04-26T18:04:35Z | |
dc.date.available | 2021-04-26T18:04:35Z | |
dc.date.issued | 2005 | |
dc.identifier.issn | 0021-8561 | |
dc.identifier.uri | http://fiver.ifvcns.rs/handle/123456789/289 | |
dc.description.abstract | A total of 832 samples of soybeans were screened by near-infrared (NIR) reflectance spectroscopy, to identify soybean samples with a lower content of oligosaccharides and nonstarch polysaccharides (NSP). Of these, 38 samples were identified on the basis of variation in protein content and agronomic value and submitted to high-resolution NIR spectroscopy. On the basis of the NIR data, 12 samples were further selected for chromatographic characterization of carbohydrate composition (mono-, di-, and oligosaccharides and NSP). Their soluble proteins were separated by two-dimensional gel electrophoresis (2DE). Using partial least-squares regression (PLSR), it was possible to predict the content of total NSP from the high-resolution NIR spectra, suggesting that NIR is a suitable and rapid nondestructive method to determine carbohydrate composition in soybeans. The 2DE analyses showed varying intensities of several proteins, including the glycinin G1 precursor. PLSR analysis showed a negative correlation between this protein and insoluble NSP and total uronic acid (UA). | en |
dc.publisher | Amer Chemical Soc, Washington | |
dc.rights | restrictedAccess | |
dc.source | Journal of Agricultural & Food Chemistry | |
dc.subject | near-infrared spectroscopy | en |
dc.subject | soybean | en |
dc.subject | Glycine max | en |
dc.subject | carbohydrate composition | en |
dc.subject | proteomics | en |
dc.subject | nonstarch polysaccharides | en |
dc.title | Evaluation of nonstarch polysaccharides and oligosaccharide content of different soybean varieties (Glycine max) by near-infrared spectroscopy and proteomics | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 9121 | |
dc.citation.issue | 23 | |
dc.citation.other | 53(23): 9112-9121 | |
dc.citation.rank | aM21 | |
dc.citation.spage | 9112 | |
dc.citation.volume | 53 | |
dc.identifier.doi | 10.1021/jf051438r | |
dc.identifier.pmid | 16277410 | |
dc.identifier.scopus | 2-s2.0-28444476301 | |
dc.identifier.wos | 000233306700035 | |
dc.type.version | publishedVersion |