Utilisation of sodium dodecyl sulphate sedimentation test for quality prediction of wheat cultivars in Serbia

Abstract

Utilisation of small-scale, high-throughput methods for wheat quality prediction is important for wheat breeding programs. Namely, fast and reliable methods are necessary for quality evaluation of a high number of genotypes from early generation. Methods that use wholegrain or wholegrain meal are developed for smaller samples, which possess additional advantage over the methods that use wheat flour. Sodium dodecyl sulphate sedimentation (SDSS) is a traditional inexpensive chemical test for preliminary prediction of the gluten quality. It is used worldwide for examination of wheat quality, but very rarely in Serbia. Therefore, the aim of this study was to investigate a possibility of using SDSS on Serbian wheat cultivars. Thirty different wheat cultivars belonging to different quality groups released by the Institute of Field and Vegetable Crops, Novi Sad were examined in the study using the SDSS method by Pena et al. (1990) with one modification. Namely, Perten DISC mill FN AB 30 was used for preparation of wheat samples instead of UDY Cyclone mill. The obtained results were opposite to the literature data.