Screening of the presence of Ol gene in NS sunflower collection

Abstract

Providing high quality oil is of great interest for oil companies. When it comes to sunflower oil, there are two types of oil on the market: high linoleic and high oleic. High oleic oil is considered a healthier version of oil, since it rich in omega-9 fatty acids that are oxidative more stable than linoleic fatty acid (omega-6 fatty acid), dominant in common sunflower oil. Development of high oleic sunflower genotypes was enabled by the discovery of Pervenets mutant sunflower population. In the IFVCNS, there is a great collection of sunflower inbred lines with wide range of oleic acid content (OAC). From the collection, we have chosen 62 genotypes for determination of OAC. In addition we used molecular marker reported by Schuppert et al. (2006) to screen for presence of the mutation that led to increase in OAC. The OAC in lines in which the presence of the mutation was detected ranged between 36.48 and 88.61% (mutant lines derived from high oleic line L31 – 36.48 – 56.58 and standard inbred lines 58.25 – 88.61%); while in lines where OAC varied between 14.24 and 34.46% this mutation was not detected. These results will help in choosing the best parental lines in future breeding programs, while the marker used will enable quick detection of the mutation. In addition it showed that the mutation in mutant lines most likely did not affect the analyzed part of the FAD2-1D sequence.