Efficiency of different molecular markers in detection of Ogu-INRA cms and Rfo genes in NS rapeseed

Abstract

Development of rapeseed hybrids was enabled by the discovery and introduction of cms and Rf genes. Several cms genes have been discovered so far: Pol, Nap, Cam, Ogu, Nsa, Shan 2A and Kos. Ogu-INRA cms, derived from Raphanus sativus, is one of the most widely analyzed and used gene in rapeseed breeding. This gene, together with compatible Rfo gene (derived from line R2000), is currently being introduced in NS breeding material. During the process of development of cms and Rf lines, some deviations from expected segregation ratio were observed. In order to analyze NS breeding material, the efficiency of four markers, two for detection of Ogu-INRA cms gene and two for detection of Rfo gene, was tested. Both markers used for detection of Ogu-INRA cms gene produced similar and comparable results, as was the case with two markers, Bn-RFO-AS2 and Boon, used for detection of Rfo gene.